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Viruses 2017, 9(2), 36;

A3R Phage and Staphylococcus aureus Lysate Do Not Induce Neutrophil Degranulation

Department of Clinical Immunology, Transplantation Institute, Medical University of Warsaw, Nowogrodzka Str. 59, 02-006 Warsaw, Poland
Laboratory of Bacteriophages, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, RudolfaWeigla Str. 12, 53-114 Wrocław, Poland
Department of Immunology, University of Warsaw, Miecznikowa Str. 1, 00-001 Warsaw, Poland
Author to whom correspondence should be addressed.
Academic Editor: Rob Lavigne
Received: 18 December 2016 / Revised: 9 February 2017 / Accepted: 15 February 2017 / Published: 21 February 2017
(This article belongs to the Section Bacterial Viruses)
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The objective of this study was to evaluate the effects of A3R phage and Staphylococcus aureus lysate obtained after phage infection on neutrophil degranulation. The exocytosis of primary and secondary granules from neutrophils was investigated in vitro in whole blood specimens by flow cytometry based on the expression of specific markers of exocytosis (CD63 for primary granules and CD66b for secondary granules). We found that both A3R and S. aureus lysate had no significant effect on the exocytosis of primary and secondary granules. These data suggest that neither A3R virions nor any products of phage-induced lysis of S. aureus are likely to induce neutrophil degranulation in patients who are treated with phage preparations. Since neutrophil granules contain some potentially toxic proteins, our results provide an important argument for the safety of phage therapy. Moreover, these data indicate that the induction of neutrophil degranulation is not likely to contribute to antibacterial effects of phages. View Full-Text
Keywords: phage; A3R; lysate; phage therapy; neutrophil; granule; degranulation; exocytosis phage; A3R; lysate; phage therapy; neutrophil; granule; degranulation; exocytosis

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Borysowski, J.; Międzybrodzki, R.; Wierzbicki, P.; Kłosowska, D.; Korczak-Kowalska, G.; Weber-Dąbrowska, B.; Górski, A. A3R Phage and Staphylococcus aureus Lysate Do Not Induce Neutrophil Degranulation. Viruses 2017, 9, 36.

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