The Influence of E1A C-Terminus on Adenovirus Replicative Cycle
Abstract
:1. Introduction
2. Materials and Methods
2.1. Antibodies
2.2. Cell and Virus Culture
2.3. EdU Incorporation Assay
2.4. Immunofluorescence
2.5. PCR Primers
2.6. Real-Time Gene Expression Analysis
2.7. Statistical Analysis
2.8. Viral Genome Quantification
2.9. Virus Growth Assay
3. Results
3.1. Growth of C-Terminus Deletion Mutants in Arrested Fibroblasts
3.2. Viral Protein and Gene Expression
3.3. Sub-Cellular Localization of Mutant E1A Proteins
3.4. Viral Genome Replication
3.5. Effects of E1A C-Terminus Mutations on S-Phase Induction
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Crisostomo, L.; Soriano, A.M.; Frost, J.R.; Olanubi, O.; Mendez, M.; Pelka, P. The Influence of E1A C-Terminus on Adenovirus Replicative Cycle. Viruses 2017, 9, 387. https://doi.org/10.3390/v9120387
Crisostomo L, Soriano AM, Frost JR, Olanubi O, Mendez M, Pelka P. The Influence of E1A C-Terminus on Adenovirus Replicative Cycle. Viruses. 2017; 9(12):387. https://doi.org/10.3390/v9120387
Chicago/Turabian StyleCrisostomo, Leandro, Andrea Michelle Soriano, Jasmine Rae Frost, Oladunni Olanubi, Megan Mendez, and Peter Pelka. 2017. "The Influence of E1A C-Terminus on Adenovirus Replicative Cycle" Viruses 9, no. 12: 387. https://doi.org/10.3390/v9120387