Mx Is Not Responsible for the Antiviral Activity of Interferon-α against Japanese Encephalitis Virus
AbstractMx proteins are interferon (IFN)-induced dynamin-like GTPases that are present in all vertebrates and inhibit the replication of myriad viruses. However, the role Mx proteins play in IFN-mediated suppression of Japanese encephalitis virus (JEV) infection is unknown. In this study, we set out to investigate the effects of Mx1 and Mx2 expression on the interferon-α (IFNα) restriction of JEV replication. To evaluate whether the inhibitory activity of IFNα on JEV is dependent on Mx1 or Mx2, we knocked down Mx1 or Mx2 with siRNA in IFNα-treated PK-15 cells and BHK-21 cells, then challenged them with JEV; the production of progeny virus was assessed by plaque assay, RT-qPCR, and Western blotting. Our results demonstrated that depletion of Mx1 or Mx2 did not affect JEV restriction imposed by IFNα, although these two proteins were knocked down 66% and 79%, respectively. Accordingly, expression of exogenous Mx1 or Mx2 did not change the inhibitory activity of IFNα to JEV. In addition, even though virus-induced membranes were damaged by Brefeldin A (BFA), overexpressing porcine Mx1 or Mx2 did not inhibit JEV proliferation. We found that BFA inhibited JEV replication, not maturation, suggesting that BFA could be developed into a novel antiviral reagent. Collectively, our findings demonstrate that IFNα inhibits JEV infection by Mx-independent pathways. View Full-Text
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Zhou, J.; Wang, S.-Q.; Wei, J.-C.; Zhang, X.-M.; Gao, Z.-C.; Liu, K.; Ma, Z.-Y.; Chen, P.-Y.; Zhou, B. Mx Is Not Responsible for the Antiviral Activity of Interferon-α against Japanese Encephalitis Virus. Viruses 2017, 9, 5.
Zhou J, Wang S-Q, Wei J-C, Zhang X-M, Gao Z-C, Liu K, Ma Z-Y, Chen P-Y, Zhou B. Mx Is Not Responsible for the Antiviral Activity of Interferon-α against Japanese Encephalitis Virus. Viruses. 2017; 9(1):5.Chicago/Turabian Style
Zhou, Jing; Wang, Shi-Qi; Wei, Jian-Chao; Zhang, Xiao-Min; Gao, Zhi-Can; Liu, Ke; Ma, Zhi-Yong; Chen, Pu-Yan; Zhou, Bin. 2017. "Mx Is Not Responsible for the Antiviral Activity of Interferon-α against Japanese Encephalitis Virus." Viruses 9, no. 1: 5.
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