Mx Is Not Responsible for the Antiviral Activity of Interferon-α against Japanese Encephalitis Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells, Virus, and Interferon
2.2. Virus Infection and Titration
2.3. Antibodies
2.4. Plasmids
2.5. Immunofluorescence Assay
2.6. Western Blot Analysis
2.7. Brefeldin A (BFA) Treatment
2.8. Knockdown Experiments
2.9. RT-qPCR
2.10. Statistical Analysis
3. Results
3.1. Mx Proteins Were Not Detectable in JEV-Infected Cells
3.2. Exogenous Mx Proteins Have No Anti-JEV Activity in Infected Cells
3.3. Mx1 or Mx2 Depletion Did Not Affect the Antiviral Activity of IFN
3.4. Mx Does Not Inhibit JEV Replication in Cells Treated with BFA
3.5. BFA Effectively Inhibits JEV Replication
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Primer | Sequence (5′ → 3′) |
---|---|
pEGFP-poMx2-F | TGACAAGCTTACCATGCCTAAACCCCGCATGTCG |
pEGFP-poMx2-R | TGACCTCGAGTTACCCCTGTAATGACTGAGC |
pcDNA3.0-poMx2-F | TGACAAGCTTACCATGCCTAAACCCCGCATGTCG |
pcDNA3.0-poMx2-R | TGACCTCGAGTTAAGCGTAGTCTGGGACGTCGTATGGGTAC CCCTGTAATGACTGAGC |
pEGFP-huMxA-F | TGACCTCGAGCTACCATGGTTGTTTCCGAAGTGGACATC |
pEGFP-huMxA-R | TGACGGATCCACCGGGGAACTGGGCAAGCCGGCG |
pEGFP-mmMx1-F | TGACCTCGAGCTACCATGGATTCTGTGAATAATCTGTGC |
pEGFP-mmMx1-R | TGACGGATCCATCGGAGAATTTGGCAAGCTTCTG |
pcDNA3.0-poMx1-F | TGACAAGCTTACCATGGTTTATTCCAGCTGTG |
pcDNA3.0-poMx1-R | TGACCTCGAGTTAAGCGTAGTCTGGGACGTCGTATGGGTAGC CTGGGAACTTGGCGA |
pcDNA3.0-poTMx1-F | TGACAAGCTTACCATGGACAAGGAGTTCCTGGAGGCTCCTAA GAAGAAGAGAAAGGTTGAGTTCAGAATTGTTTATTCC |
AACTGTGAAAGTAAAGAACCTGATTCAGTT | |
pcDNA3.0-poTMx1-R | TGACCTCGAGTTAAGCGTAGTCTGGGACGTCGTATGGGTAGCC TGGGAACTTGGCGA |
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Zhou, J.; Wang, S.-Q.; Wei, J.-C.; Zhang, X.-M.; Gao, Z.-C.; Liu, K.; Ma, Z.-Y.; Chen, P.-Y.; Zhou, B. Mx Is Not Responsible for the Antiviral Activity of Interferon-α against Japanese Encephalitis Virus. Viruses 2017, 9, 5. https://doi.org/10.3390/v9010005
Zhou J, Wang S-Q, Wei J-C, Zhang X-M, Gao Z-C, Liu K, Ma Z-Y, Chen P-Y, Zhou B. Mx Is Not Responsible for the Antiviral Activity of Interferon-α against Japanese Encephalitis Virus. Viruses. 2017; 9(1):5. https://doi.org/10.3390/v9010005
Chicago/Turabian StyleZhou, Jing, Shi-Qi Wang, Jian-Chao Wei, Xiao-Min Zhang, Zhi-Can Gao, Ke Liu, Zhi-Yong Ma, Pu-Yan Chen, and Bin Zhou. 2017. "Mx Is Not Responsible for the Antiviral Activity of Interferon-α against Japanese Encephalitis Virus" Viruses 9, no. 1: 5. https://doi.org/10.3390/v9010005