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Viruses 2016, 8(8), 214;

Fluorescent and Bioluminescent Reporter Myxoviruses

Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30307, USA
Children’s Healthcare of Atlanta, 1405 Clifton Road, Atlanta, GA 30322, USA
Author to whom correspondence should be addressed.
Academic Editor: Luis Martinez-Sobrido
Received: 10 May 2016 / Revised: 8 July 2016 / Accepted: 11 July 2016 / Published: 4 August 2016
(This article belongs to the Special Issue Replication-Competent Reporter-Expressing Viruses)
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The advent of virus reverse genetics has enabled the incorporation of genetically encoded reporter proteins into replication-competent viruses. These reporters include fluorescent proteins which have intrinsic chromophores that absorb light and re-emit it at lower wavelengths, and bioluminescent proteins which are luciferase enzymes that react with substrates to produce visible light. The incorporation of these reporters into replication-competent viruses has revolutionized our understanding of molecular virology and aspects of viral tropism and transmission. Reporter viruses have also enabled the development of high-throughput assays to screen antiviral compounds and antibodies and to perform neutralization assays. However, there remain technical challenges with the design of replication-competent reporter viruses, and each reporter has unique advantages and disadvantages for specific applications. This review describes currently available reporters, design strategies for incorporating reporters into replication-competent paramyxoviruses and orthomyxoviruses, and the variety of applications for which these tools can be utilized both in vitro and in vivo. View Full-Text
Keywords: fluorescent reporter virus; bioluminescent reporter virus; paramyxovirus; orthomyxovirus fluorescent reporter virus; bioluminescent reporter virus; paramyxovirus; orthomyxovirus

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Rostad, C.A.; Currier, M.C.; Moore, M.L. Fluorescent and Bioluminescent Reporter Myxoviruses. Viruses 2016, 8, 214.

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