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PKR Activation Favors Infectious Pancreatic Necrosis Virus Replication in Infected Cells

Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, P.O. Box 8146 Dep., 0033 Oslo, Norway
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Academic Editor: Andrew Mehle
Viruses 2016, 8(6), 173; https://doi.org/10.3390/v8060173
Received: 22 April 2016 / Revised: 31 May 2016 / Accepted: 3 June 2016 / Published: 21 June 2016
(This article belongs to the Section Animal Viruses)
The double-stranded RNA-activated protein kinase R (PKR) is a Type I interferon (IFN) stimulated gene that has important biological and immunological functions. In viral infections, in general, PKR inhibits or promotes viral replication, but PKR-IPNV interaction has not been previously studied. We investigated the involvement of PKR during infectious pancreatic necrosis virus (IPNV) infection using a custom-made rabbit antiserum and the PKR inhibitor C16. Reactivity of the antiserum to PKR in CHSE-214 cells was confirmed after IFNα treatment giving an increased protein level. IPNV infection alone did not give increased PKR levels by Western blot, while pre-treatment with PKR inhibitor before IPNV infection gave decreased eukaryotic initiation factor 2-alpha (eIF2α) phosphorylation. This suggests that PKR, despite not being upregulated, is involved in eIF2α phosphorylation during IPNV infection. PKR inhibitor pre-treatment resulted in decreased virus titers, extra- and intracellularly, concomitant with reduction of cells with compromised membranes in IPNV-permissive cell lines. These findings suggest that IPNV uses PKR activation to promote virus replication in infected cells. View Full-Text
Keywords: PKR; cloning; PKR inhibitor; IPNV; eIF2α phosphorylation; necrosis PKR; cloning; PKR inhibitor; IPNV; eIF2α phosphorylation; necrosis
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MDPI and ACS Style

Gamil, A.A.; Xu, C.; Mutoloki, S.; Evensen, Ø. PKR Activation Favors Infectious Pancreatic Necrosis Virus Replication in Infected Cells. Viruses 2016, 8, 173.

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