Next Article in Journal
Re-Designed Recombinant Hepatitis B Virus Vectors Enable Efficient Delivery of Versatile Cargo Genes to Hepatocytes with Improved Safety
Next Article in Special Issue
Use of Reporter Genes in the Generation of Vaccinia Virus-Derived Vectors
Previous Article in Journal
Divergent Trends of Anti-JCPyV Serum Reactivity and Neutralizing Activity in Multiple Sclerosis (MS) Patients during Treatment with Natalizumab
Previous Article in Special Issue
Applications of Replicating-Competent Reporter-Expressing Viruses in Diagnostic and Molecular Virology
Article Menu

Export Article

Open AccessReview
Viruses 2016, 8(5), 125;

Engineering Hepadnaviruses as Reporter-Expressing Vectors: Recent Progress and Future Perspectives

Key Laboratory of Medical Molecular Virology (MOH & MOE) and Institutes of Biomedical Sciences, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editor: Luis Martinez-Sobrido
Received: 29 February 2016 / Revised: 21 April 2016 / Accepted: 29 April 2016 / Published: 10 May 2016
(This article belongs to the Special Issue Replication-Competent Reporter-Expressing Viruses)
Full-Text   |   PDF [704 KB, uploaded 10 May 2016]   |  


The Hepadnaviridae family of small, enveloped DNA viruses are characterized by a strict host range and hepatocyte tropism. The prototype hepatitis B virus (HBV) is a major human pathogen and constitutes a public health problem, especially in high-incidence areas. Reporter-expressing recombinant viruses are powerful tools in both studies of basic virology and development of antiviral therapeutics. In addition, the highly restricted tropism of HBV for human hepatocytes makes it an ideal tool for hepatocyte-targeting in vivo applications such as liver-specific gene delivery. However, compact genome organization and complex replication mechanisms of hepadnaviruses have made it difficult to engineer replication-competent recombinant viruses that express biologically-relevant cargo genes. This review analyzes difficulties associated with recombinant hepadnavirus vector development, summarizes and compares the progress made in this field both historically and recently, and discusses future perspectives regarding both vector design and application. View Full-Text
Keywords: recombinant virus; viral vector; gene delivery; HBV; DHBV recombinant virus; viral vector; gene delivery; HBV; DHBV

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Printed Edition Available!
A printed edition of this Special Issue is available here.

Share & Cite This Article

MDPI and ACS Style

Bai, W.; Cui, X.; Xie, Y.; Liu, J. Engineering Hepadnaviruses as Reporter-Expressing Vectors: Recent Progress and Future Perspectives. Viruses 2016, 8, 125.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top