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Review

Human Cytomegalovirus Strategies to Maintain and Promote mRNA Translation

by
Heather A. Vincent
,
Benjamin Ziehr
and
Nathaniel J. Moorman
*
Department of Microbiology & Immunology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
*
Author to whom correspondence should be addressed.
Viruses 2016, 8(4), 97; https://doi.org/10.3390/v8040097
Submission received: 1 March 2016 / Revised: 25 March 2016 / Accepted: 31 March 2016 / Published: 13 April 2016
(This article belongs to the Special Issue Viral Subversion of Stress Responses and Translational Control)

Abstract

:
mRNA translation requires the ordered assembly of translation initiation factors and ribosomal subunits on a transcript. Host signaling pathways regulate each step in this process to match levels of protein synthesis to environmental cues. In response to infection, cells activate multiple defenses that limit viral protein synthesis, which viruses must counteract to successfully replicate. Human cytomegalovirus (HCMV) inhibits host defenses that limit viral protein expression and manipulates host signaling pathways to promote the expression of both host and viral proteins necessary for virus replication. Here we review key regulatory steps in mRNA translation, and the strategies used by HCMV to maintain protein synthesis in infected cells.

1. Introduction

Viruses are completely reliant on the host translation machinery for the synthesis of viral proteins, since no virus encodes a ribosome. As a result, viral and host mRNAs must compete for access to ribosomes. Viruses must also counteract host defenses that inactivate the translation machinery after sensing viral infection. Human cytomegalovirus (HCMV) effectively antagonizes host defenses that limit viral protein expression. In addition, HCMV manipulates multiple host signaling pathways to ensure the continued synthesis of both host and viral proteins needed for virus replication. Thus, the interface between viral mRNAs and the host translation machinery serves as a critical determinant for successful HCMV infection. The purpose of this review is to summarize key steps in the regulation of mRNA translation, and strategies by which HCMV manipulates the host translation machinery to benefit virus replication.

2. The Scanning Model of Translation Initiation

Translation of mRNAs occurs in three steps: initiation, elongation and termination [1,2]. Translation initiation begins with the assembly of the eukaryotic initiation factor 4F (eIF4F) translation initiation complex on the 7-methyl guanosine cap (m7G cap) at the 5’ end of the mRNA. ([3,4,5] reviewed in [2]). The bound eIF4F complex recruits the 43S preinitiation complex (PIC), consisting of the 40S ribosomal subunit, the ternary complex and multiple initiation factors, to form the 48S initiation complex. The 48S complex then scans the 5’ untranslated region (UTR) of the mRNA until reaching the translation start codon, whereupon multiple initiation factors are released and the 60S ribosomal subunit is recruited [6]. Joining of the 40S and 60S ribosomal subunits to form the 80S ribosome marks the end of the initiation phase, and the beginning of elongation. Elongation is regulated by the eukaryotic elongation factor 1 (eEF1), which promotes binding of aminoacyl-tRNAs to the A-site of the ribosome, and eukaryotic elongation factor 2 (eEF2), which facilitates the translocation of peptidyl-tRNA from the A-site to the P-site of the ribosome [7,8,9]. Peptide elongation continues until a translation termination codon is encountered, whereupon translation ceases, the nascent peptide chain is displaced from the ribosome, and the ribosome disassembles [10,11]. The first step of translation, initiation, is the most regulated step ([12,13,14] reviewed in [15]) and is described in more detail below.
Formation of the eIF4F complex, which consists of eukaryotic initiation factors 4E (eIF4E), 4G (eIF4G) and 4A (eIF4A), bound to the 5’ m7G cap of an mRNA mediates the initiation of translation [2,16]. eIF4F assembly begins with binding of eIF4E to the m7G cap [3,17]. After binding the m7G cap, eIF4E recruits eIF4G, which acts as a scaffold protein that mediates the recruitment of the eIF4A RNA helicase, completing the assembly of the eIF4F complex [18,19]. eIF4G also coordinates the interaction of the eIF4F complex with additional factors bound to the mRNA, such as the poly(A) binding protein (PABP), that enhance translation initiation [2,16,18,19,20].
Prior to binding the eIF4F complex, multiple initiation factors associate with the 40S ribosomal subunit to prepare for translation initiation. Together, the 40S subunit and its associated factors are referred to as the 43S PIC ([21,22] reviewed in [23]). The 43S PIC contains the 40S ribosomal subunit, multiple initiation factors (e.g., eIF1, eIF1A, eIF3, and eIF5), and a ternary complex composed of the eukaryotic initiation factor 2 (eIF2), guanosine triphosphate (GTP) and a charged methionyl-tRNA (tRNAMet) [24]. eIF3 plays a critical role during 43S assembly, acting as a scaffold to recruit multiple initiation factors to the 40S ribosomal subunit [25]. These factors affect ribosome scanning, eIF4A helicase activity, and fidelity of start codon recognition once associated with the eIF4F complex on the 5’UTR of the mRNA [25,26].
Recruitment of the 43S PIC to the mRNA is mediated via an interaction between the eIF4G subunit of the eIF4F complex and eIF3 within the 43S PIC [27]. Together, the 43S PIC, the eIF4F complex, and the bound mRNA constitute the 48S initiation complex. Once assembled, the 48S complex scans the 5’UTR of the mRNA until reaching the translation “start” codon [28]. The eIF4A helicase, whose activity is stimulated by binding to eIF4B [29], unwinds RNA structures that would otherwise impede 48S scanning [28,30]. Additional RNA helicases such as DDX3, DHX9 and DHX29 also facilitate scanning through areas with high RNA secondary structure [31,32,33,34]. Scanning ceases upon recognition of a translation start site, which almost always consists of an AUG methionine codon in a favorable sequence context (i.e., Kozak sequence [35]). The combined actions of the eIF1, eIF1A and eIF5 initiation factors position the tRNAMet over the AUG codon, triggering hydrolysis of eIF2-bound GTP and release of a subset of initiation factors [6]. eIF5 directs subsequent joining of the 40S and 60S ribosomal subunits to form a functional 80S ribosome, after which peptide elongation commences [36]. Elongation continues until a translation termination or “stop” codon is encountered, at which point eukaryotic release factor 1 (eRF-1) together with eukaryotic release factor 3 (eRF-3) terminates elongation by displacing the nascent peptide from the ribosome [10,11].

3. Alternative Translation Initiation Mechanisms

Translation of some viral mRNAs does not require 40S ribosomal subunit scanning. Instead, ribosomes are recruited to the site of translation initiation, often with limited or no ribosome scanning, by specific RNA sequences/structures called internal ribosome entry sites (IRESs) ([37,38] reviewed in [39]). Ribosome recruitment to IRESs often requires only a subset of translation initiation factors. For example, translation initiation from the poliovirus IRES is independent of the eIF4E cap binding protein, but requires eIF4A, eIF4G and the 43S PIC [40]. On the other end of the spectrum, the cricket paralysis virus (CrPV) IRES requires only 40S and 60S ribosomal subunits to initiate translation [41,42]. Although these and other IRESs require differing factors to initiate translation, RNA secondary and tertiary structure is indispensable for the recruitment of ribosomal subunits to IRES elements [43].
In addition to initiation factors, IRES activity can be enhanced through binding of IRES trans-activating factors, or ITAFs [44]. Two cellular factors consistently function as ITAFs, the polypyrimidine tract binding protein (PTB) and the lupus autoantigen (La). PTB is an RNA binding protein that directly interacts with RNA secondary structures to promote RNA folding and maintain IRES structures [45,46]. The La protein binds the 5’UTR of multiple viruses, including poliovirus, human immunodeficiency virus (HIV), hepatitis C virus (HCV), and influenza virus [47]. Binding of La to IRES sequences stimulates recruitment of the 40S ribosomal subunit, possibly to promote correct start codon usage [48]. Both proteins are critical for the activity of multiple viral IRESs ([49,50] reviewed in [51]) and promote IRES driven translation during HCV, poliovirus and rhinovirus infection [52,53].

4. Signaling Pathways Regulating Translation Initiation

4.1. mTOR Signaling

The target of rapamycin (TOR) kinase is conserved throughout eukaryotes, where it plays a critical role in modulating protein synthesis in response to the intracellular and extracellular environment ([54,55] reviewed in [1]). In mammalian cells, mTOR (mammalian TOR) is found in two complexes: the mTORC1 and mTORC2 complexes [56,57]. While the two complexes share several subunits, each complex has unique defining components. mTORC1 contains the protein regulatory-associated protein of mTOR (RAPTOR), while the presence of rapamycin-insensitive companion of mTOR (RICTOR) defines the mTORC2 complex [58,59]. mTORC1 plays a central role in regulating translation initiation by controlling the assembly of the eIF4F complex [60]. In the hypo-phosphorylated state, the eIF4E-binding protein 1 (4EBP1) binds eIF4E and prevents binding to eIF4G, thereby limiting eIF4F formation [61]. Phosphorylation of 4EBP1 by mTORC1 reduces the affinity of 4EBP1 for eIF4E, allowing efficient eIF4F complex formation and increasing overall levels of protein synthesis (Figure 1A) [62].
mTORC1 also promotes protein synthesis by phosphorylating and activating the 70 kDa ribosomal protein S6 kinase (p70S6K). Active p70S6K phosphorylates several factors involved in translation, such as eIF4B and eukaryotic elongation factor 2 kinase (eEF2K) [63,64]. When phosphorylated, eIF4B binds eIF4A and increases its helicase activity, resulting in more efficient 48S scanning through 5’UTRs with significant secondary structure [65]. Like mTORC1 itself, p70S6K also phosphorylates and deactivates a translation repressor, eEFK2 [64,66]. eEFK2 phosphorylates and inactivates eukaryotic elongation factor 2 (eEF2), which stimulates the incorporation of amino acids into the growing peptide chain [67,68]. eEF2K phosphorylation of eEF2 prevents its association with the ribosome, thereby slowing the rate of elongation [7]. Thus, protein synthesis is induced through multiple mechanisms upon mTORC1 activation.
Protein synthesis is one of the most energy intensive cellular processes. Therefore mTORC1 is unsurprisingly subject to both positive and negative regulation in response to environmental cues. This regulation converges on the tuberous sclerosis complex (TSC), which negatively regulates mTORC1 activity [69,70]. The heterodimeric TSC consists of the tuberous sclerosis 1 (TSC1) and tuberous sclerosis 2 (TSC2) proteins that together act as a GTPase activating protein (GAP) for the mTORC1 cofactor Rheb [71]. mTORC1 is activated by association with Rheb-GTP and repressed by Rheb-GDP. Increased TSC GAP activity stimulates hydrolysis of GTP bound to Rheb, and therefore inhibits mTORC1 activity [72]. Decreased nutrient availability leads to elevated AMP to ATP ratios that activate the AMP-regulated protein kinase (AMPK) [73]. Active AMPK phosphorylates TSC2, stimulating TSC GAP activity and inhibiting mTORC1 activity [74]. Conversely, when nutrients are plentiful, growth factor receptor signaling activates the phosphoinositide 3-kinase (PI3K), which phosphorylates and inhibits the TSC complex, thereby promoting mTORC1 activity and eIF4F assembly [75]. Thus the TSC complex integrates upstream signaling pathways to modulate mTORC1 activity and protein synthesis to match the nutrient availability within the cellular environment.

4.2. eIF2α Kinase Activation

Another focal point in the regulation of translation initiation is the phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2α). eIF2α is part of the trimeric eIF2 complex, which together with GTP and tRNAMet, forms the ternary complex associated with the 43S PIC [76]. Recognition of the AUG initiation codon by tRNAMet stimulates the hydrolysis of eIF2-associated GTP. The resulting release of the free phosphate triggers eIF2-GDP release from the ribosome [77]. Eukaryotic initiation factor 2B (eIF2B) exchanges GDP for GTP in the eIF2 complex, allowing eIF2-GTP to form a new ternary complex and participate in subsequent rounds of initiation [78]. Phosphorylation of eIF2α by one of four eIF2α kinases (see below) greatly increases the affinity of eIF2B for eIF2, preventing eIF2B release and GTP exchange [79,80]. As eIF2α is present at a much greater concentration than eIF2B, even small increases in eIF2α phosphorylation can sequester essentially all of the available eIF2B, preventing the formation of new ternary complexes and resulting in a dramatic decrease in protein synthesis [81].
Similar to the mTOR signaling pathway, eIF2α kinases regulate protein synthesis in response to the intracellular environment. Four eIF2α kinases have been identified, each of which are activated in response to a distinct cellular stress (reviewed in [82]). The heme-regulated inhibitor (HRI) kinase phosphorylates eIF2α in response to high levels of reactive oxygen species, linking the rate of protein synthesis to the respiratory capacity of the cell [83]. Accumulation of unfolded proteins within the endoplasmic reticulum (ER) activates the PKR-like endoplasmic reticulum kinase (PERK) [84], temporarily inhibiting protein synthesis to allow the cell to properly fold or degrade accumulated unfolded proteins. The eIF2α kinase general control nonderepressible 2 (GCN2) is activated by binding to uncharged tRNAs that accumulate during amino acid deprivation, directly linking amino acid availability to the rate of protein synthesis [85,86]. Especially relevant to viral infections, protein kinase R (PKR) is activated by binding to double stranded RNAs (dsRNAs) produced during infection [87,88,89,90], and potently inhibits viral protein synthesis, and thus virus replication [80].

5. HCMV Manipulation of Translation Initiation

Unlike many viruses, HCMV does not limit host translation. Instead, overall levels of protein synthesis are maintained in HCMV-infected cells [91]. As both host and viral mRNAs rely on the same pool of ribosomes for their translation, HCMV mRNAs must efficiently compete with host transcripts for access to the translation machinery to ensure synthesis of viral proteins. In addition, the virus must circumvent antiviral defenses that otherwise limit protein synthesis upon infection. HCMV infection activates both antiviral defenses and stress response pathways, yet viral protein synthesis and replication remain unaffected (see below). While the full spectrum of strategies employed by HCMV to regulate host responses is beyond the scope of this review, examples of the strategies HCMV uses to maintain viral protein synthesis by counteracting host defenses and stress responses are described below.

5.1 HCMV Infection Increases eIF4F Abundance and Activity during Infection

As discussed above, the eIF4F complex plays a critical role in translation initiation by recruiting the 43S PIC to the 5’ end of the mRNA. Recruitment of the eIF4F complex, particularly binding of the eIF4E subunit to the mRNA m7G cap, is thought to be the rate-limiting step of translation initiation [92]. Overall levels of eIF4F directly correlate with the level of protein synthesis within the cell. The sustained eIF4F-dependent translation of host mRNAs during HCMV infection suggests that the virus manipulates cellular signaling pathways to maintain eIF4F activity (Figure 1A). Consistent with this idea, Walsh et al. found that HCMV infection increases the abundance of eIF4F subunits and promotes eIF4F complex formation [91]. In addition, elevated levels of PABP stimulate eIF4F formation during infection [93]. The increase in eIF4F levels in infected cells is important for virus replication, as disrupting or inhibiting the eIF4F complex early during infection profoundly limits viral replication [94,95,96]. Similarly, an eIF4A helicase inhibitor suppresses HCMV replication in vitro when added at the time of infection [95]. This in part reflects the necessity for eIF4F-dependent translation of host mRNAs during infection, as depletion of several host proteins that require eIF4F for their expression reduced the production of progeny virus [97].
In addition to increasing the abundance of eIF4F subunits, HCMV activates signaling pathways that stimulate eIF4F complex formation. mTORC1 activity is increased in HCMV infected cells, promoting eIF4F formation through phosphorylation and inactivation of the 4EBP family of translational repressors (Figure 1A) [91,94,98]. During infection, mTORC1 activity is refractile to cellular stresses such as AMPK activation that typically decrease its activity [99,100,101]. In fact HCMV paradoxically requires increased activation of both AMPK and mTOR during infection for efficient replication [102]. Thus HCMV uncouples mTORC1 activity from negative regulatory cues to promote virus replication. The dichotomy of continued mTORC1 activity despite AMPK activation can be explained, in part, by the finding that the HCMV UL38 protein (pUL38) binds and inhibits the host TSC2 protein, preventing inhibition of mTORC1 in response to nutrient deprivation and AMPK agonists [103]. pUL38 also stimulates mTORC1 activity in a TSC2-independent manner, although the mechanism remains unclear (Figure 1A) [104]. Thus HCMV pUL38 severs the connection between AMPK and mTOR signaling, allowing for eIF4F formation and maintained levels of protein synthesis.
HCMV infection also stimulates additional signaling pathways that potentially enhance translation during infection. The PI3K signaling pathway is stimulated during infection and increases mTORC1 activity [105,106]. Chemical inhibitors of PI3K limit HCMV replication [105], suggesting that PI3K signaling could play a role in stimulating translation in infected cells. HCMV infection also activates the MNK kinases [91], which phosphorylate eIF4E (Figure 1A). Phosphorylation of eIF4E is suggested to increase the rate of translation through an unknown mechanism. Inhibitors of the MNK kinases reduce HCMV replication [91], suggesting that MNK-dependent eIF4E phosphorylation potentially regulates protein synthesis during infection. Infection also increases the abundance of the critical translation elongation factor eEF2 in a UL38-dependent manner (Figure 1B). While the role of the above signaling changes in HCMV translation has not been demonstrated, their association with the control of translation in other contexts suggests a potential role in translation regulation during HCMV infection.
As suggested by the multiple mechanisms HCMV employs to induce and maintain mTORC1 activity, decreased mTOR activity or expression inhibits virus replication [94,95,96]. Depletion of mTOR, RICTOR or RAPTOR decreases HCMV replication, as do ATP-competitive inhibitors of mTOR kinase activity [98,107]. When added at the time of infection, mTOR inhibitors limit viral DNA accumulation and thus the transcription of HCMV late genes. However, mTOR inhibitors also prevent metabolic remodeling induced by HCMV during infection, thus the effects of the inhibitors are likely pleiotropic [108]. Although mTOR inhibitors limit virus replication when added at the start of infection, such drugs have little effect on viral protein synthesis, the association of viral mRNAs with polysomes or virus replication when added later in infection [96,107].

5.2. IRES Activity during HCMV Infection

Many viruses use IRES elements to ensure translation of viral mRNAs under stress conditions that limit host protein synthesis. The only IRES-like element identified to date in the HCMV genome is located adjacent to the UL138 open reading frame (ORF). The UL138 ORF is the most 3’ of four ORFs encoded on a polycistronic mRNA, suggesting that cap-mediated translation initiation of UL138 would be exceedingly inefficient. The UL138 IRES-like element directs internal initiation on the polycistronic pUL138 transcript and allows for increased UL138 protein synthesis under conditions of cell stress [109]. While the role of the UL138 IRES during infection is unknown, it likely plays a role in regulating HCMV latency, as pUL138 acts as a molecular switch that regulates virus reactivation [110]. Whether or not HCMV encodes additional IRES-like elements that allow for non-canonical translation initiation events remains to be determined.
HCMV infection also stimulates the translation of at least one IRES-containing cellular mRNA needed for virus replication. Cells induce a coordinated response to the accumulation of unfolded proteins in the ER, aptly named the unfolded protein response (UPR). While induction of the UPR generally suppresses protein synthesis, the translation of a subset of mRNAs involved in resolving cell stress is selectively increased. While HCMV infection induces the UPR, downstream signaling pathways are selectively modulated to support virus replication [111,112]. One example is the increased IRES-dependent translation of the ER chaperone BiP (binding immunoglobulin protein) during infection, which likely supports HCMV replication by increasing the protein folding capacity of the ER [113,114]. HCMV stimulates BiP mRNA translation in part by increasing the expression of the La protein, a known ITAF for the BiP IRES [115]. Given the increase in La abundance during infection and the wide range of cellular and viral mRNAs that La interacts with, HCMV infection may stimulate the translation of additional IRES-containing mRNAs.

5.3. HCMV Regulation of eIF2α Kinases

HCMV infection generates cellular stresses that are potent activators of eIF2α kinases. Of the eIF2α kinases, the role of the antiviral PKR during infection is the best characterized. PKR is activated upon binding to double stranded RNAs (dsRNAs) generated during the early stage of HCMV infection [116]. Yet PKR activation and eIF2α phosphorylation are not observed at this time, suggesting HCMV actively prevents PKR activation. Using a screen to identify HCMV genes that rescue growth of a vaccinia virus mutant lacking its PKR antagonist E3L, the laboratory of Dr. Adam Geballe identified the HCMV TRS1 and IRS1 proteins (pTRS1 and pIRS1, respectively) as potent PKR antagonists [117]. Expression of either pTRS1 or pIRS1 is necessary for HCMV infection, as infection in the absence of both proteins results in an almost complete block to virus replication [116]. The first two thirds of TRS1 and IRS1 are encoded by the terminal repeats flanking the unique short region of the HCMV genome, and thus the first two thirds of pTRS1 and pIRS1 are identical [118]. This common amino-terminal region contains an unusual RNA binding domain [119], which functions to competitively inhibit PKR by binding to dsRNA ligands [120]. Both pTRS1 and pIRS1 also contain a PKR binding domain in their divergent C-terminal region [121]. The RNA and PKR binding domains of pTRS1 are both necessary to rescue replication of a vaccinia virus lacking the E3L protein [119,120]. However, the physical interaction with PKR appears to be most important for PKR inhibition during HCMV infection, as mutation or deletion of the pTRS1 PKR binding domain results in PKR activation in the absence of pIRS1 [122,123]. When PKR is depleted or deleted prior to infection HCMV replication is restored in the absence of pTRS1 and pIRS1 [122,123], suggesting that a major function of pTRS1/pIRS1 is to counteract inhibition of viral protein synthesis by PKR (Figure 1B).
Despite pTRS1/pIRS1 inhibition of PKR, phosphorylated eIF2α accumulates in infected cells during the late stage of HCMV infection [111,124]. Mammalian cells express three additional eIF2α kinases, PERK, HRI and GCN2 whose activation could account for the observed increase in eIF2α phosphorylation. While the potential role of HRI and GCN2 in HCMV-induced eIF2α phosphorylation is unknown, PERK is not the relevant eIF2α kinase as phosphorylated eIF2α accumulates during infection of PERK-depleted cells [112]. In any case, both host and viral mRNAs are translated despite the observed increase in eIF2α phosphorylation late in infection. It is currently unclear if eIF2α phosphorylation no longer restricts translation initiation late in infection, or if the extent of eIF2α phosphorylation is insufficient to suppress protein synthesis. Perhaps the increased abundance and activity of the host PP1 and PP2A phosphatases during infection [125] in conjunction with viral inhibitors of eIF2α kinases allow for efficient translation in the presence of multiple eIF2α stresses. Regardless, continued protein synthesis in the face of significant cellular stress suggests that HCMV encodes viral proteins that limit eIF2α kinase activity, or actively limit eIF2α phosphorylation in response to virus-induced stress.

6. Unresolved Questions and Future Directions

As described above, HCMV employs a variety of strategies to counteract host defenses and maximize the translation of viral mRNAs. However, several recent studies suggest that our understanding of HCMV manipulation of the host translation machinery is incomplete. One unresolved question concerns the role of the eIF4F complex in HCMV protein synthesis. Disrupting or inhibiting the eIF4F complex at the start of infection decreases HCMV replication [94,95,96], correlating with a decrease in the translation efficiency of several host mRNAs needed for progression through the viral lytic cycle [97]. Yet as infection progresses, viral replication becomes increasingly resistant to mTOR inhibitors, even though these inhibitors continue to efficiently disrupt eIF4F complex formation [107]. Despite significantly reduced eIF4F abundance, HCMV mRNAs efficiently associate with polysomes even though the translation of several host mRNAs is simultaneously decreased [95]. How are viral mRNAs efficiently translated under eIF4F-limiting conditions? Perhaps the relatively high abundance of HCMV transcripts with structurally simple 5’UTRs allows them to compete efficiently for ribosomes when eIF4F is limiting. Another possibility is that a host or viral factor recruits residual eIF4F specifically to HCMV mRNAs. This may in part explain the finding that HCMV mRNAs as a group are more efficiently translated than host mRNAs during the later stages of infection [126]. Alternatively, an unknown host or viral factor might functionally substitute for components of the eIF4F complex. This could be a host factor that similarly facilitates translation of cellular mRNAs under eIF4F-limiting conditions. In any case, these results suggest that translation initiation on HCMV mRNAs differs from that on eIF4F-dependent host mRNAs. Exploring the mechanisms underlying this difference will likely expand our understanding of both cellular and viral translation mechanisms.
Another emerging question in the regulation of HCMV translation stems from the recent discovery that the coding potential of the viral genome has been greatly underestimated. Ribosome profiling (next generation sequencing of ribosome-protected mRNA fragments) of HCMV infected cells combined with mass spectrometry revealed that HCMV encodes over 750 polypeptides [127], as compared to previous estimates of approximately 200 protein coding regions [128,129]. How does HCMV encode such a wide array of peptides, and what is their function? In some cases the use of a near-cognate initiation codon drives translation from previously unrecognized ORFs, although the majority of the novel coding regions initiate with an AUG start codon. Many of the novel peptides were less than 30 amino acids in length, and therefore may not function as proteins in the commonly understood sense. However, short peptides encoded upstream of longer coding regions on the same mRNA can serve regulatory roles to modulate mRNA translation in response to the cellular environment. Small upstream open reading frames, or uORFs, are translation competent ORFs consisting of fewer than 30 codons located 5’ of a protein coding region on the same mRNA ([130,131] reviewed in [132]). Under normal conditions ribosomes initiate translation at the first AUG codon they encounter in a favorable sequence context (e.g., Kozak sequence [35]), and therefore translate uORFs rather than the downstream protein coding region. However, during periods of stress that limit ternary complex availability, uORFs can counterintuitively stimulate translation of the downstream coding region [133] through a poorly defined mechanism. Therefore these short, translation competent HCMV ORFs could act as translational regulators for adjacent protein coding regions, providing the virus a means to ensure continued viral protein expression under conditions of cellular stress.
Many new coding regions were also found internal or antisense to previously annotated ORFs, raising the question of how these novel ORFs are translated. Is each peptide-coding region contained on its own transcript? Or might HCMV extensively utilize polycistronic mRNAs to expand its coding capacity? Based on the relative paucity of confirmed IRES elements in herpesvirus genomes, it seems unlikely that internal ribosome entry can explain the diversity of the viral translatome. A more likely explanation for the diversity of viral polypeptides is that the complexity of the HCMV transcriptome has also been underestimated. Both strands of the viral genome are extensively transcribed [134,135,136], potentially explaining the presence of “antisense” coding regions in some ORFs. HCMV mRNA splicing is also more extensive than once thought [134], and alternative transcription start site (TSS) usage in some cases extends or truncates known reading frames [136]. Such extensive transcriptome complexity suggests that each HCMV polypeptide may arise from cap-dependent translation of a monocistronic mRNA, rather than through IRES-dependent translation of polycistronic messages. A more thorough characterization of the HCMV transcriptome will therefore shed light on the mechanisms regulating the translation of the expansive HCMV proteome.
While we know much about the control of mRNA translation during HCMV infection, much remains to be learned. The growing list of high-resolution techniques available to study protein synthesis should allow for a more complete picture of regulatory events controlling both host and viral mRNA translation during HCMV infection. Given the growing appreciation of the importance of translation regulation in multiple disease states, this knowledge may lead to new targets for novel therapeutics that inhibit viral protein synthesis, and thereby decrease HCMV disease.

Acknowledgments

We wish to thank members of the Moorman and Heise labs for helpful discussions, and Anne Beall for comments on the manuscript. This work was supported by National Institutes of Health (NIH) grant RO1 AI103311 to N.J.M. B.Z. was supported by funds from the University of North Carolina Virology Training grant T32 AI007419.

Author Contributions

H.A.V., B.Z. and N.J.M. wrote the paper.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

The following abbreviations are used in this manuscript:
HCMVhuman cytomegalovirus
eIF4Feukaryotic initiation factor 4F
m7G cap7-methyl guanosine cap
43S PIC43S preinitiation complex
UTRuntranslated region
eEF1eukaryotic elongation factor 1
eEF2eukaryotic elongation factor 2
eIF4Eeukaryotic initiation factor 4E
eIF4Geukaryotic initiation factor 4G
eIF4Aeukaryotic initiation factor 4A
PABPpoly(A) binding protein
eIF1eukaryotic initiation factor 1
eIF1Aeukaryotic initiation factor 1A
eIF3eukaryotic initiation factor 3
eIF4Beukaryotic initiation factor 4B
eIF5eukaryotic initiation factor 5
eIF2eukaryotic initiation factor 2
tRNAMetmethionyl-tRNA
eRF-1eukaryotic release factor 1
eRF-3eukaryotic release factor 3
IRESInternal Ribosome Entry Sites
ITAFIRES trans-activating factor
PTBpolypyrimidine tract binding protein
Lalupus autoantigen
HIVhuman immunodeficiency virus
HCVhepatitis C virus
mTORmammalian target of rapamycin kinase
4EBP1eIF4E-binding protein 1
p70S6K70 kDa ribosomal protein S6 kinase
eEF2Keukaryotic elongation factor 2 kinase
TSCtuberous sclerosis complex
TSC1tuberous sclerosis complex 1
TSC2tuberous sclerosis complex 2
GAPGTPase activating protein
AMPKAMP-regulated protein kinase
PI3Kphosphoinositide 3-kinase
AKTprotein kinase B
eIF2αeukaryotic initiation factor 2 alpha subunit
eIF2Beukaryotic initiation factor 2B
HRIheme-regulated inhibitor
ERendoplasmic reticulum
PERKPKR-like endoplasmic reticulum kinase
GCN2general control nonderepressible 2
PKRprotein kinase R
dsRNAdouble stranded RNA
UPRunfolded protein response
BiPbinding immunoglobulin protein
ORFopen reading frame
uORFupstream open reading frame
TSStranscription start site

References

  1. Gingras, A.C.; Raught, B.; Sonenberg, N. Regulation of translation initiation by FRAP/mTOR. Genes Dev. 2001, 15, 807–826. [Google Scholar] [CrossRef] [PubMed]
  2. Gingras, A.C.; Raught, B.; Sonenberg, N. eIF4 initiation factors: Effectors of mRNA recruitment to ribosomes and regulators of translation. Annu. Rev. Biochem. 1999, 68, 913–963. [Google Scholar] [CrossRef] [PubMed]
  3. Tahara, S.M.; Morgan, M.A.; Shatkin, A.J. Two forms of purified m7G-cap binding protein with different effects on capped mRNA translation in extracts of uninfected and poliovirus-infected HeLa cells. J. Biol. Chem. 1981, 256, 7691–7694. [Google Scholar] [PubMed]
  4. Grifo, J.A.; Tahara, S.M.; Morgan, M.A.; Shatkin, A.J.; Merrick, W.C. New initiation factor activity required for globin mRNA translation. J. Biol. Chem. 1983, 258, 5804–5810. [Google Scholar] [PubMed]
  5. Sonenberg, N.; Shatkin, A.J. Nonspecific effect of m7GMP on protein-RNA interactions. J. Biol. Chem. 1978, 253, 6630–6632. [Google Scholar] [PubMed]
  6. Thomas, A.; Spaan, W.; van Steeg, H.; Voorma, H.O.; Benne, R. Mode of action of protein synthesis initiation factor eIF-1 from rabbit reticulocytes. FEBS Lett. 1980, 116, 67–71. [Google Scholar] [CrossRef]
  7. Carlberg, U.; Nilsson, A.; Nygard, O. Functional properties of phosphorylated elongation factor 2. Eur. J. Biochem. 1990, 191, 639–645. [Google Scholar] [CrossRef] [PubMed]
  8. Gao, Y.G.; Selmer, M.; Dunham, C.M.; Weixlbaumer, A.; Kelley, A.C.; Ramakrishnan, V. The structure of the ribosome with elongation factor G trapped in the posttranslocational state. Science 2009, 326, 694–699. [Google Scholar] [CrossRef] [PubMed]
  9. Kimata, Y.; Kohno, K. Elongation factor 2 mutants deficient in diphthamide formation show temperature-sensitive cell growth. J. Biol. Chem. 1994, 269, 13497–13501. [Google Scholar] [PubMed]
  10. Stansfield, I.; Kushnirov, V.V.; Jones, K.M.; Tuite, M.F. A conditional-lethal translation termination defect in a sup45 mutant of the yeast Saccharomyces cerevisiae. Eur. J. Biochem. 1997, 245, 557–563. [Google Scholar] [CrossRef] [PubMed]
  11. Zhouravleva, G.; Frolova, L.; Le Goff, X.; Le Guellec, R.; Inge-Vechtomov, S.; Kisselev, L.; Philippe, M. Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3. EMBO J. 1995, 14, 4065–4072. [Google Scholar] [PubMed]
  12. De Benedetti, A.; Rhoads, R.E. Overexpression of eukaryotic protein synthesis initiation factor 4E in HeLa cells results in aberrant growth and morphology. Proc. Natl. Acad. Sci. USA 1990, 87, 8212–8216. [Google Scholar] [CrossRef] [PubMed]
  13. Fan, H.; Penman, S. Regulation of protein synthesis in mammalian cells. J. Mol. Biol. 1970, 50, 655–670. [Google Scholar] [CrossRef]
  14. Singer, R.H.; Penman, S. Biological sciences: Stability of HeLa cell mRNA in actinomycin. Nature 1972, 240, 100–102. [Google Scholar] [CrossRef] [PubMed]
  15. Jackson, R.J.; Hellen, C.U.; Pestova, T.V. The mechanism of eukaryotic translation initiation and principles of its regulation. Nat. Rev. Mol. Cell Biol. 2010, 11, 113–127. [Google Scholar] [CrossRef] [PubMed]
  16. Imataka, H.; Sonenberg, N. Human eukaryotic translation initiation factor 4G (eIF4G) possesses two separate and independent binding sites for eIF4A. Mol. Cell. Biol. 1997, 17, 6940–6947. [Google Scholar] [CrossRef] [PubMed]
  17. Sonenberg, N.; Trachsel, H.; Hecht, S.; Shatkin, A.J. Differential stimulation of capped mRNA translation in vitro by cap binding protein. Nature 1980, 285, 331–333. [Google Scholar] [CrossRef] [PubMed]
  18. Marcotrigiano, J.; Gingras, A.C.; Sonenberg, N.; Burley, S.K. Cap-dependent translation initiation in eukaryotes is regulated by a molecular mimic of eiIF4G. Mol. Cell 1999, 3, 707–716. [Google Scholar] [CrossRef]
  19. Slepenkov, S.V.; Korneeva, N.L.; Rhoads, R.E. Kinetic mechanism for assembly of the m7GPPPG.eIF4E.eIF4GE complex. J. Biol. Chem. 2008, 283, 25227–25237. [Google Scholar] [CrossRef] [PubMed]
  20. Hinton, T.M.; Coldwell, M.J.; Carpenter, G.A.; Morley, S.J.; Pain, V.M. Functional analysis of individual binding activities of the scaffold protein eIF4G. J. Biol. Chem. 2007, 282, 1695–1708. [Google Scholar] [CrossRef] [PubMed]
  21. Shin, B.-S.; Kim, J.-R.; Walker, S.E.; Dong, J.; Lorsch, J.R.; Dever, T.E. Initiation factor eIF2γ promotes eIF2–GTP–Met-tRNAMet ternary complex binding to the 40S ribosome. Nat. Struct. Mol. Biol. 2011, 18, 1227–1234. [Google Scholar] [CrossRef] [PubMed]
  22. Methot, N.; Song, M.S.; Sonenberg, N. A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self-association and interaction with eIF3. Mol. Cell Biol. 1996, 16, 5328–5334. [Google Scholar] [CrossRef] [PubMed]
  23. Hinnebusch, A.G.; Lorsch, J.R. The mechanism of eukaryotic translation initiation: New insights and challenges. Cold Spring Harb. Perspect. Biol. 2012, 4. [Google Scholar] [CrossRef] [PubMed]
  24. Asano, K.; Clayton, J.; Shalev, A.; Hinnebusch, A.G. A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo. Genes Dev. 2000, 14, 2534–2546. [Google Scholar] [CrossRef] [PubMed]
  25. Valásek, L.; Nielsen, K.H.; Zhang, F.; Fekete, C.A.; Hinnebusch, A.G. Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection. Mol. Cell. Biol. 2004, 24, 9437–9455. [Google Scholar] [CrossRef] [PubMed]
  26. Rozovsky, N.; Butterworth, A.C.; Moore, M.J. Interactions between eIF4AI and its accessory factors eIF4B and eIF4H. RNA 2008, 14, 2136–2148. [Google Scholar] [CrossRef] [PubMed]
  27. LeFebvre, A.K.; Korneeva, N.L.; Trutschl, M.; Cvek, U.; Duzan, R.D.; Bradley, C.A.; Hershey, J.W.B.; Rhoads, R.E. Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3E subunit. J. Biol. Chem. 2006, 281, 22917–22932. [Google Scholar] [CrossRef] [PubMed]
  28. Kozak, M. Influence of mRNA secondary structure on binding and migration of 40S ribosomal subunits. Cell 1980, 19, 79–90. [Google Scholar] [CrossRef]
  29. Parsyan, A.; Svitkin, Y.; Shahbazian, D.; Gkogkas, C.; Lasko, P.; Merrick, W.C.; Sonenberg, N. MRNA helicases: The tacticians of translational control. Nat. Rev. Mol. Cell Biol. 2011, 12, 235–245. [Google Scholar] [CrossRef] [PubMed]
  30. Berthelot, K.; Muldoon, M.; Rajkowitsch, L.; Hughes, J.; McCarthy, J.E.G. Dynamics and processivity of 40S ribosome scanning on mRNA in yeast. Mol. Microbiol. 2004, 51, 987–1001. [Google Scholar] [CrossRef] [PubMed]
  31. Parsyan, A.; Shahbazian, D.; Martineau, Y.; Petroulakis, E.; Alain, T.; Larsson, O.; Mathonnet, G.; Tettweiler, G.; Hellen, C.U.; Pestova, T.V.; et al. The helicase protein DHX29 promotes translation initiation, cell proliferation, and tumorigenesis. Proc. Natl. Acad. Sci. USA 2009, 106, 22217–22222. [Google Scholar] [CrossRef] [PubMed]
  32. Lee, C.-S.; Dias, A.P.; Jedrychowski, M.; Patel, A.H.; Hsu, J.L.; Reed, R. Human DDX3 functions in translation and interacts with the translation initiation factor eIF3. Nucleic Acids Res. 2008, 36, 4708–4718. [Google Scholar] [CrossRef] [PubMed]
  33. Hartman, T.R.; Qian, S.; Bolinger, C.; Fernandez, S.; Schoenberg, D.R.; Boris-Lawrie, K. RNA helicase A is necessary for translation of selected messenger RNAs. Nat. Struct. Mol. Biol. 2006, 13, 509–516. [Google Scholar] [CrossRef] [PubMed]
  34. Pisareva, V.P.; Pisarev, A.V.; Komar, A.A.; Hellen, C.U.T.; Pestova, T.V. Translation initiation on mammalian mRNAs with structured 5′UTRs requires DExH-box protein DHX29. Cell 2008, 135, 1237–1250. [Google Scholar] [CrossRef] [PubMed]
  35. Kozak, M. An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987, 15, 8125–8148. [Google Scholar] [CrossRef] [PubMed]
  36. Pestova, T.V.; Lomakin, I.B.; Lee, J.H.; Choi, S.K.; Dever, T.E.; Hellen, C.U. The joining of ribosomal subunits in eukaryotes requires eIF5B. Nature 2000, 403, 332–335. [Google Scholar] [CrossRef] [PubMed]
  37. Jang, S.K.; Kräusslich, H.G.; Nicklin, M.J.; Duke, G.M.; Palmenberg, A.C.; Wimmer, E. A segment of the 5′ nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. J. Virol. 1988, 62, 2636–2643. [Google Scholar] [PubMed]
  38. Pelletier, J.; Sonenberg, N. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature 1988, 334, 320–325. [Google Scholar] [CrossRef] [PubMed]
  39. Jackson, R.J. Alternative mechanisms of initiating translation of mammalian mRNAs. Biochem. Soc. Trans. 2005, 33, 1231–1241. [Google Scholar] [CrossRef] [PubMed]
  40. Sweeney, T.R.; Abaeva, I.S.; Pestova, T.V.; Hellen, C.U.T. The mechanism of translation initiation on type 1 picornavirus IRESs. EMBO J. 2014, 33, 76–92. [Google Scholar] [CrossRef] [PubMed]
  41. Schüler, M.; Connell, S.R.; Lescoute, A.; Giesebrecht, J.; Dabrowski, M.; Schroeer, B.; Mielke, T.; Penczek, P.A.; Westhof, E.; Spahn, C.M.T. Structure of the ribosome-bound cricket paralysis virus IRES RNA. Nat. Struct. Mol. Biol. 2006, 13, 1092–1096. [Google Scholar] [CrossRef] [PubMed]
  42. Spahn, C.M.T.; Jan, E.; Mulder, A.; Grassucci, R.A.; Sarnow, P.; Frank, J. Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: The IRES functions as an RNA-based translation factor. Cell 2004, 118, 465–475. [Google Scholar] [CrossRef] [PubMed]
  43. Kieft, J.S. Viral IRES RNA structures and ribosome interactions. Trends Biochem. Sci. 2008, 33, 274–283. [Google Scholar] [CrossRef] [PubMed]
  44. Komar, A.A.; Hatzoglou, M. Cellular IRES-mediated translation: The war of ITAFs in pathophysiological states. Cell Cycle 2011, 10, 229–240. [Google Scholar] [CrossRef] [PubMed]
  45. Gil, A.; Sharp, P.A.; Jamison, S.F.; Garcia-Blanco, M.A. Characterization of cDNAs encoding the polypyrimidine tract-binding protein. Genes Dev. 1991, 5, 1224–1236. [Google Scholar] [CrossRef] [PubMed]
  46. Mitchell, S.A.; Spriggs, K.A.; Coldwell, M.J.; Jackson, R.J.; Willis, A.E. The APAF-1 internal ribosome entry segment attains the correct structural conformation for function via interactions with PTB and UNR. Mol. Cell 2003, 11, 757–771. [Google Scholar] [CrossRef]
  47. Izumi, R.E.; Das, S.; Barat, B.; Raychaudhuri, S.; Dasgupta, A. A peptide from autoantigen La blocks poliovirus and hepatitis C virus cap-independent translation and reveals a single tyrosine critical for La RNA binding and translation stimulation. J. Virol. 2004, 78, 3763–3776. [Google Scholar] [CrossRef] [PubMed]
  48. McBratney, S.; Sarnow, P. Evidence for involvement of trans-acting factors in selection of the AUG start codon during eukaryotic translational initiation. Mol. Cell. Biol. 1996, 16, 3523–3534. [Google Scholar] [CrossRef] [PubMed]
  49. Kaminski, A.; Hunt, S.L.; Patton, J.G.; Jackson, R.J. Direct evidence that polypyrimidine tract binding protein (PTB) is essential for internal initiation of translation of encephalomyocarditis virus RNA. RNA 1995, 1, 924–938. [Google Scholar] [PubMed]
  50. Costa-Mattioli, M.; Svitkin, Y.; Sonenberg, N. La autoantigen is necessary for optimal function of the poliovirus and hepatitis C virus internal ribosome entry site in vivo and in vitro. Mol. Cell. Biol. 2004, 24, 6861–6870. [Google Scholar] [CrossRef] [PubMed]
  51. Fitzgerald, K.D.; Semler, B.L. Bridging IRES elements in mRNAs to the eukaryotic translation apparatus. Biochim. Biophys. Acta 2009, 1789, 518–528. [Google Scholar] [CrossRef] [PubMed]
  52. Svitkin, Y.V.; Meerovitch, K.; Lee, H.S.; Dholakia, J.N.; Kenan, D.J.; Agol, V.I.; Sonenberg, N. Internal translation initiation on poliovirus RNA: Further characterization of La function in poliovirus translation in vitro. J. Virol. 1994, 68, 1544–1550. [Google Scholar] [PubMed]
  53. Hunt, S.L.; Jackson, R.J. Polypyrimidine-tract binding protein (PTB) is necessary, but not sufficient, for efficient internal initiation of translation of human rhinovirus-2 RNA. RNA 1999, 5, 344–359. [Google Scholar] [CrossRef] [PubMed]
  54. Lin, T.A.; Kong, X.; Haystead, T.A.; Pause, A.; Belsham, G.; Sonenberg, N.; Lawrence, J.C. Phas-I as a link between mitogen-activated protein kinase and translation initiation. Science 1994, 266, 653–656. [Google Scholar] [CrossRef] [PubMed]
  55. Jefferies, H.B.; Fumagalli, S.; Dennis, P.B.; Reinhard, C.; Pearson, R.B.; Thomas, G. Rapamycin suppresses 5′TOP mRNA translation through inhibition of p70s6k. EMBO J. 1997, 16, 3693–3704. [Google Scholar] [CrossRef] [PubMed]
  56. Loewith, R.; Jacinto, E.; Wullschleger, S.; Lorberg, A.; Crespo, J.L.; Bonenfant, D.; Oppliger, W.; Jenoe, P.; Hall, M.N. Two tor complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol. Cell 2002, 10, 457–468. [Google Scholar] [CrossRef]
  57. Helliwell, S.B.; Wagner, P.; Kunz, J.; Deuter-Reinhard, M.; Henriquez, R.; Hall, M.N. TOR1 and TOR2 are structurally and functionally similar but not identical phosphatidylinositol kinase homologues in yeast. Mol. Biol. Cell 1994, 5, 105–118. [Google Scholar] [CrossRef] [PubMed]
  58. Kim, D.-H.; Sarbassov, D.D.; Ali, S.M.; King, J.E.; Latek, R.R.; Erdjument-Bromage, H.; Tempst, P.; Sabatini, D.M. mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Cell 2002, 110, 163–175. [Google Scholar] [CrossRef]
  59. Sarbassov, D.D.; Ali, S.M.; Kim, D.-H.; Guertin, D.A.; Latek, R.R.; Erdjument-Bromage, H.; Tempst, P.; Sabatini, D.M. Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and RAPTOR-independent pathway that regulates the cytoskeleton. Curr. Biol. 2004, 14, 1296–1302. [Google Scholar] [CrossRef] [PubMed]
  60. Thoreen, C.C. Many roads from mTOR to eIF4F. Biochem. Soc. Trans. 2013, 41, 913–916. [Google Scholar] [CrossRef] [PubMed]
  61. Pause, A.; Belsham, G.J.; Gingras, A.C.; Donzé, O.; Lin, T.A.; Lawrence, J.C.; Sonenberg, N. Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5′-CAP function. Nature 1994, 371, 762–767. [Google Scholar] [CrossRef] [PubMed]
  62. Fadden, P.; Haystead, T.A.; Lawrence, J.C. Identification of phosphorylation sites in the translational regulator, phas-I that are controlled by insulin and rapamycin in rat adipocytes. J. Biol. Chem. 1997, 272, 10240–10247. [Google Scholar] [PubMed]
  63. Shahbazian, D.; Roux, P.P.; Mieulet, V.; Cohen, M.S.; Raught, B.; Taunton, J.; Hershey, J.W.B.; Blenis, J.; Pende, M.; Sonenberg, N. The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity. EMBO J. 2006, 25, 2781–2791. [Google Scholar] [CrossRef] [PubMed]
  64. Faller, W.J.; Jackson, T.J.; Knight, J.R.P.; Ridgway, R.A.; Jamieson, T.; Karim, S.A.; Jones, C.; Radulescu, S.; Huels, D.J.; Myant, K.B.; et al. MTORC1-mediated translational elongation limits intestinal tumour initiation and growth. Nature 2015, 517, 497–500. [Google Scholar] [CrossRef] [PubMed]
  65. Ozes, A.R.; Feoktistova, K.; Avanzino, B.C.; Fraser, C.S. Duplex unwinding and atpase activities of the dead-box helicase eIF4A are coupled by eIF4G and eIF4B. J. Mol. Biol. 2011, 412, 674–687. [Google Scholar] [PubMed]
  66. Wang, X.; Li, W.; Williams, M.; Terada, N.; Alessi, D.R.; Proud, C.G. Regulation of elongation factor 2 kinase by p90RSK1 and p70 S6 kinase. EMBO J. 2001, 20, 4370–4379. [Google Scholar] [CrossRef] [PubMed]
  67. Wang, L.; Wang, X.; Proud, C.G. Activation of mRNA translation in rat cardiac myocytes by insulin involves multiple rapamycin-sensitive steps. Am. J. Physiol. Heart Circ. Physiol. 2000, 278, H1056–H1068. [Google Scholar] [PubMed]
  68. Redpath, N.T.; Foulstone, E.J.; Proud, C.G. Regulation of translation elongation factor-2 by insulin via a rapamycin-sensitive signalling pathway. EMBO J. 1996, 15, 2291–2297. [Google Scholar] [PubMed]
  69. Gao, X.; Pan, D. TSC1 and TSC2 tumor suppressors antagonize insulin signaling in cell growth. Genes Dev. 2001, 15, 1383–1392. [Google Scholar] [CrossRef] [PubMed]
  70. Manning, B.D.; Cantley, L.C. Rheb fills a GAP between TSC and TOR. Trends Biochem. Sci. 2003, 28, 573–576. [Google Scholar] [CrossRef] [PubMed]
  71. Saucedo, L.J.; Gao, X.; Chiarelli, D.A.; Li, L.; Pan, D.; Edgar, B.A. Rheb promotes cell growth as a component of the insulin/tor signalling network. Nat. Cell Biol. 2003, 5, 566–571. [Google Scholar] [CrossRef] [PubMed]
  72. Inoki, K.; Li, Y.; Xu, T.; Guan, K.-L. Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling. Genes Dev. 2003, 17, 1829–1834. [Google Scholar] [CrossRef] [PubMed]
  73. Hardie, D.G.; Schaffer, B.E.; Brunet, A. AMPK: An energy-sensing pathway with multiple inputs and outputs. Trends Cell Biol. 2016, 26, 190–201. [Google Scholar] [CrossRef] [PubMed]
  74. Inoki, K.; Zhu, T.; Guan, K.-L. TSC2 mediates cellular energy response to control cell growth and survival. Cell 2003, 115, 577–590. [Google Scholar] [CrossRef]
  75. Manning, B.D.; Tee, A.R.; Logsdon, M.N.; Blenis, J.; Cantley, L.C. Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/AKT pathway. Mol. Cell 2002, 10, 151–162. [Google Scholar] [CrossRef]
  76. Sonenberg, N.; Hinnebusch, A.G. Regulation of translation initiation in eukaryotes: Mechanisms and biological targets. Cell 2009, 136, 731–745. [Google Scholar] [CrossRef] [PubMed]
  77. Algire, M.A.; Maag, D.; Lorsch, J.R. Pi release from eIF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation. Mol. Cell 2005, 20, 251–262. [Google Scholar] [CrossRef] [PubMed]
  78. Flynn, A.; Oldfield, S.; Proud, C.G. The role of the beta-subunit of initiation factor eIF-2 in initiation complex formation. Biochim. Biophys. Acta 1993, 1174, 117–121. [Google Scholar] [CrossRef]
  79. Pavitt, G.D.; Ramaiah, K.V.; Kimball, S.R.; Hinnebusch, A.G. EIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange. Genes Dev. 1998, 12, 514–526. [Google Scholar] [CrossRef] [PubMed]
  80. Krishnamoorthy, T.; Pavitt, G.D.; Zhang, F.; Dever, T.E.; Hinnebusch, A.G. Tight binding of the phosphorylated alpha subunit of initiation factor 2 (eIF2alpha) to the regulatory subunits of guanine nucleotide exchange factor eIF2B is required for inhibition of translation initiation. Mol. Cell. Biol. 2001, 21, 5018–5030. [Google Scholar] [CrossRef] [PubMed]
  81. Singh, C.R.; Udagawa, T.; Lee, B.; Wassink, S.; He, H.; Yamamoto, Y.; Anderson, J.T.; Pavitt, G.D.; Asano, K. Change in nutritional status modulates the abundance of critical pre-initiation intermediate complexes during translation initiation in vivo. J. Mol. Biol. 2007, 370, 315–330. [Google Scholar] [CrossRef] [PubMed]
  82. Wek, R.C.; Jiang, H.Y.; Anthony, T.G. Coping with stress: eIF2 kinases and translational control. Biochem. Soc. Trans. 2006, 34, 7–11. [Google Scholar] [CrossRef] [PubMed]
  83. Lu, L.; Han, A.P.; Chen, J.J. Translation initiation control by heme-regulated eukaryotic initiation factor 2alpha kinase in erythroid cells under cytoplasmic stresses. Mol. Cell. Biol. 2001, 21, 7971–7980. [Google Scholar] [CrossRef] [PubMed]
  84. Walter, P.; Ron, D. The unfolded protein response: From stress pathway to homeostatic regulation. Science 2011, 334, 1081–1086. [Google Scholar] [CrossRef] [PubMed]
  85. Dever, T.E.; Feng, L.; Wek, R.C.; Cigan, A.M.; Donahue, T.F.; Hinnebusch, A.G. Phosphorylation of initiation factor 2 alpha by protein kinase GCN2 mediates gene-specific translational control of GCN4 in yeast. Cell 1992, 68, 585–596. [Google Scholar] [CrossRef]
  86. Dever, T.E.; Hinnebusch, A.G. GCN2 whets the appetite for amino acids. Mol. Cell 2005, 18, 141–142. [Google Scholar] [CrossRef] [PubMed]
  87. Dar, A.C.; Dever, T.E.; Sicheri, F. Higher-order substrate recognition of eIF2alpha by the RNA-dependent protein kinase PKR. Cell 2005, 122, 887–900. [Google Scholar] [CrossRef] [PubMed]
  88. Dey, M.; Cao, C.; Dar, A.C.; Tamura, T.; Ozato, K.; Sicheri, F.; Dever, T.E. Mechanistic link between PKR dimerization, autophosphorylation, and eIF2alpha substrate recognition. Cell 2005, 122, 901–913. [Google Scholar] [CrossRef] [PubMed]
  89. Romano, P.R.; Zhang, F.; Tan, S.L.; Garcia-Barrio, M.T.; Katze, M.G.; Dever, T.E.; Hinnebusch, A.G. Inhibition of double-stranded RNA-dependent protein kinase PKR by vaccinia virus E3: Role of complex formation and the E3 n-terminal domain. Mol. Cell Biol. 1998, 18, 7304–7316. [Google Scholar] [CrossRef] [PubMed]
  90. Zhang, F.; Romano, P.R.; Nagamura-Inoue, T.; Tian, B.; Dever, T.E.; Mathews, M.B.; Ozato, K.; Hinnebusch, A.G. Binding of double-stranded RNA to protein kinase PKR is required for dimerization and promotes critical autophosphorylation events in the activation loop. J. Biol. Chem. 2001, 276, 24946–24958. [Google Scholar] [CrossRef] [PubMed]
  91. Walsh, D.; Perez, C.; Notary, J.; Mohr, I. Regulation of the translation initiation factor eIF4F by multiple mechanisms in human cytomegalovirus-infected cells. J. Virol. 2005, 79, 8057–8064. [Google Scholar] [CrossRef] [PubMed]
  92. Pestova, T.V.; Kolupaeva, V.G.; Lomakin, I.B.; Pilipenko, E.V.; Shatsky, I.N.; Agol, V.I.; Hellen, C.U. Molecular mechanisms of translation initiation in eukaryotes. Proc. Natl. Acad. Sci. USA 2001, 98, 7029–7036. [Google Scholar] [CrossRef] [PubMed]
  93. McKinney, C.; Perez, C.; Mohr, I. Poly(A) binding protein abundance regulates eukaryotic translation initiation factor 4F assembly in human cytomegalovirus-infected cells. Proc. Natl. Acad. Sci. USA 2012, 109, 5627–5632. [Google Scholar] [CrossRef] [PubMed]
  94. Kudchodkar, S.B.; Yu, Y.; Maguire, T.G.; Alwine, J.C. Human cytomegalovirus infection induces rapamycin-insensitive phosphorylation of downstream effectors of mTOR kinase. J. Virol. 2004, 78, 11030–11039. [Google Scholar] [CrossRef] [PubMed]
  95. Lenarcic, E.M.; Ziehr, B.; De Leon, G.; Mitchell, D.; Moorman, N.J. Differential role for host translation factors in host and viral protein synthesis during human cytomegalovirus infection. J. Virol. 2014, 88, 1473–1483. [Google Scholar] [CrossRef] [PubMed]
  96. Moorman, N.J.; Shenk, T. Rapamycin-resistant mTORC1 kinase activity is required for herpesvirus replication. J. Virol. 2010, 84, 5260–5269. [Google Scholar] [CrossRef] [PubMed]
  97. McKinney, C.; Zavadil, J.; Bianco, C.; Shiflett, L.; Brown, S.; Mohr, I. Global reprogramming of the cellular translational landscape facilitates cytomegalovirus replication. Cell Rep. 2014, 6, 9–17. [Google Scholar] [CrossRef] [PubMed]
  98. Kudchodkar, S.B.; Yu, Y.; Maguire, T.G.; Alwine, J.C. Human cytomegalovirus infection alters the substrate specificities and rapamycin sensitivities of RAPTOR- and RICTOR-containing complexes. Proc. Natl. Acad. Sci. USA 2006, 103, 14182–14187. [Google Scholar] [CrossRef] [PubMed]
  99. Clippinger, A.J.; Maguire, T.G.; Alwine, J.C. Human cytomegalovirus infection maintains mTOR activity and its perinuclear localization during amino acid deprivation. J. Virol. 2011, 85, 9369–9376. [Google Scholar] [CrossRef] [PubMed]
  100. Kudchodkar, S.B.; Del Prete, G.Q.; Maguire, T.G.; Alwine, J.C. AMPK-mediated inhibition of mTOR kinase is circumvented during immediate-early times of human cytomegalovirus infection. J. Virol. 2007, 81, 3649–3651. [Google Scholar] [CrossRef] [PubMed]
  101. Tilton, C.; Clippinger, A.J.; Maguire, T.; Alwine, J.C. Human cytomegalovirus induces multiple means to combat reactive oxygen species. J. Virol. 2011, 85, 12585–12593. [Google Scholar] [CrossRef] [PubMed]
  102. McArdle, J.; Moorman, N.J.; Munger, J. HCMV targets the metabolic stress response through activation of AMPK whose activity is important for viral replication. PLoS Pathog. 2012, 8, e1002502. [Google Scholar] [CrossRef] [PubMed]
  103. Moorman, N.J.; Cristea, I.M.; Terhune, S.S.; Rout, M.P.; Chait, B.T.; Shenk, T. Human cytomegalovirus protein UL38 inhibits host cell stress responses by antagonizing the tuberous sclerosis protein complex. Cell Host Microbe 2008, 3, 253–262. [Google Scholar] [CrossRef] [PubMed]
  104. Bai, Y.; Xuan, B.; Liu, H.; Zhong, J.; Yu, D.; Qian, Z. Tuberous sclerosis complex protein 2-independent activation of mTORC1 by human cytomegalovirus pUL38. J. Virol. 2015, 89, 7625–7635. [Google Scholar] [CrossRef] [PubMed]
  105. Johnson, R.A.; Wang, X.; Ma, X.-L.; Huong, S.-M.; Huang, E.-S. Human cytomegalovirus up-regulates the phosphatidylinositol 3-kinase (PI3-K) pathway: Inhibition of PI3-K activity inhibits viral replication and virus-induced signaling. J. Virol. 2001, 75, 6022–6032. [Google Scholar] [CrossRef] [PubMed]
  106. Buchkovich, N.J.; Yu, Y.; Zampieri, C.A.; Alwine, J.C. The torrid affairs of viruses: Effects of mammalian DNA viruses on the PI3K-AKT-mTOR signalling pathway. Nat. Rev. Microbiol. 2008, 6, 266–275. [Google Scholar] [CrossRef] [PubMed]
  107. Clippinger, A.J.; Maguire, T.G.; Alwine, J.C. The changing role of mTOR kinase in the maintenance of protein synthesis during human cytomegalovirus infection. J. Virol. 2011, 85, 3930–3939. [Google Scholar] [CrossRef] [PubMed]
  108. Spencer, C.M.; Schafer, X.L.; Moorman, N.J.; Munger, J. Human cytomegalovirus induces the activity and expression of acetyl-coenzyme A carboxylase, a fatty acid biosynthetic enzyme whose inhibition attenuates viral replication. J. Virol. 2011, 85, 5814–5824. [Google Scholar] [CrossRef] [PubMed]
  109. Grainger, L.; Cicchini, L.; Rak, M.; Petrucelli, A.; Fitzgerald, K.D.; Semler, B.L.; Goodrum, F. Stress-inducible alternative translation initiation of human cytomegalovirus latency protein pUL138. J. Virol. 2010, 84, 9472–9486. [Google Scholar] [CrossRef] [PubMed]
  110. Petrucelli, A.; Rak, M.; Grainger, L.; Goodrum, F. Characterization of a novel golgi apparatus-localized latency determinant encoded by human cytomegalovirus. J. Virol. 2009, 83, 5615–5629. [Google Scholar] [CrossRef] [PubMed]
  111. Isler, J.A.; Skalet, A.H.; Alwine, J.C. Human cytomegalovirus infection activates and regulates the unfolded protein response. J. Virol. 2005, 79, 6890–6899. [Google Scholar] [CrossRef] [PubMed]
  112. Yu, Y.; Pierciey, F.J.; Maguire, T.G.; Alwine, J.C. PKR-like endoplasmic reticulum kinase is necessary for lipogenic activation during HCMV infection. PLoS Pathog. 2013, 9, e1003266. [Google Scholar] [CrossRef] [PubMed]
  113. Buchkovich, N.J.; Maguire, T.G.; Yu, Y.; Paton, A.W.; Paton, J.C.; Alwine, J.C. Human cytomegalovirus specifically controls the levels of the endoplasmic reticulum chaperone BiP/GRP78, which is required for virion assembly. J. Virol. 2008, 82, 31–39. [Google Scholar] [CrossRef] [PubMed]
  114. Buchkovich, N.J.; Yu, Y.; Pierciey, F.J.; Alwine, J.C. Human cytomegalovirus induces the endoplasmic reticulum chaperone BiP through increased transcription and activation of translation by using the BiP internal ribosome entry site. J. Virol. 2010, 84, 11479–11486. [Google Scholar] [CrossRef] [PubMed]
  115. Kim, Y.K.; Back, S.H.; Rho, J.; Lee, S.H.; Jang, S.K. La autoantigen enhances translation of BiP mRNA. Nucleic Acids Res. 2001, 29, 5009–5016. [Google Scholar] [CrossRef] [PubMed]
  116. Marshall, E.E.; Bierle, C.J.; Brune, W.; Geballe, A.P. Essential role for either TSR1 or IRS1 in human cytomegalovirus replication. J. Virol. 2009, 83, 4112–4120. [Google Scholar] [CrossRef] [PubMed]
  117. Child, S.J.; Hakki, M.; De Niro, K.L.; Geballe, A.P. Evasion of cellular antiviral responses by human cytomegalovirus TSR1 and IRS1. J. Virol. 2004, 78, 197–205. [Google Scholar] [CrossRef] [PubMed]
  118. Romanowski, M.J.; Shenk, T. Characterization of the human cytomegalovirus IRS1 and TRS1 genes: A second immediate-early transcription unit within IRS1 whose product antagonizes transcriptional activation. J. Virol. 1997, 71, 1485–1496. [Google Scholar] [PubMed]
  119. Hakki, M.; Geballe, A.P. Double-stranded RNA binding by human cytomegalovirus PTRS1. J. Virol. 2005, 79, 7311–7318. [Google Scholar] [CrossRef] [PubMed]
  120. Bierle, C.J.; Semmens, K.M.; Geballe, A.P. Double-stranded RNA binding by the human cytomegalovirus PKR antagonist TRS1. Virology 2013, 442, 28–37. [Google Scholar] [CrossRef] [PubMed]
  121. Hakki, M.; Marshall, E.E.; De Niro, K.L.; Geballe, A.P. Binding and nuclear relocalization of protein kinase R by human cytomegalovirus TRS1. J. Virol. 2006, 80, 11817–11826. [Google Scholar] [CrossRef] [PubMed]
  122. Braggin, J.E.; Child, S.J.; Geballe, A.P. Essential role of protein kinase R antagonism by TRS1 in human cytomegalovirus replication. Virology 2016, 489, 75–85. [Google Scholar] [CrossRef] [PubMed]
  123. Ziehr, B.; Vincent, H.A.; Moorman, N.J. Human cytomegalovirus PTRS1 and PIRS1 antagonize PKR to facilitate virus replication. J. Virol. 2016. [Google Scholar] [CrossRef] [PubMed]
  124. Xuan, B.; Qian, Z.; Torigoi, E.; Yu, D. Human cytomegalovirus protein pUL38 induces ATF4 expression, inhibits persistent JNK phosphorylation, and suppresses endoplasmic reticulum stress-induced cell death. J. Virol. 2009, 83, 3463–3474. [Google Scholar] [CrossRef] [PubMed]
  125. Hakki, M.; Geballe, A.P. Cellular serine/threonine phosphatase activity during human cytomegalovirus infection. Virology 2008, 380, 255–263. [Google Scholar] [CrossRef] [PubMed]
  126. Tirosh, O.; Cohen, Y.; Shitrit, A.; Shani, O.; Le-Trilling, V.T.; Trilling, M.; Friedlander, G.; Tanenbaum, M.; Stern-Ginossar, N. The transcription and translation landscapes during human cytomegalovirus infection reveal novel host-pathogen interactions. PLoS Pathog. 2015, 11, e1005288. [Google Scholar] [CrossRef] [PubMed]
  127. Stern-Ginossar, N.; Weisburd, B.; Michalski, A.; Le, V.T.K.; Hein, M.Y.; Huang, S.-X.; Ma, M.; Shen, B.; Qian, S.-B.; Hengel, H.; et al. Decoding human cytomegalovirus. Science 2012, 338, 1088–1093. [Google Scholar] [CrossRef] [PubMed]
  128. Chee, M.S.; Bankier, A.T.; Beck, S.; Bohni, R.; Brown, C.M.; Cerny, R.; Horsnell, T.; Hutchison, C.A., 3rd; Kouzarides, T.; Martignetti, J.A.; et al. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr. Top. Microbiol. Immunol. 1990, 154, 125–169. [Google Scholar] [PubMed]
  129. Murphy, E.; Shenk, T. Human cytomegalovirus genome. Curr. Top. Microbiol. Immunol. 2008, 325, 1–19. [Google Scholar] [PubMed]
  130. Calvo, S.E.; Pagliarini, D.J.; Mootha, V.K. Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans. Proc. Natl. Acad. Sci. USA 2009, 106, 7507–7512. [Google Scholar] [CrossRef] [PubMed]
  131. Matsui, M.; Yachie, N.; Okada, Y.; Saito, R.; Tomita, M. Bioinformatic analysis of post-transcriptional regulation by uORF in human and mouse. FEBS Lett. 2007, 581, 4184–4188. [Google Scholar] [CrossRef] [PubMed]
  132. Wethmar, K. The regulatory potential of upstream open reading frames in eukaryotic gene expression. Wiley Interdiscip. Rev. RNA 2014, 5, 765–778. [Google Scholar] [CrossRef] [PubMed]
  133. Watatani, Y.; Ichikawa, K.; Nakanishi, N.; Fujimoto, M.; Takeda, H.; Kimura, N.; Hirose, H.; Takahashi, S.; Takahashi, Y. Stress-induced translation of ATF5 mRNA is regulated by the 5′-untranslated region. J. Biol. Chem. 2008, 283, 2543–2553. [Google Scholar] [CrossRef] [PubMed]
  134. Gatherer, D.; Seirafian, S.; Cunningham, C.; Holton, M.; Dargan, D.J.; Baluchova, K.; Hector, R.D.; Galbraith, J.; Herzyk, P.; Wilkinson, G.W.; et al. High-resolution human cytomegalovirus transcriptome. Proc. Natl. Acad. Sci. USA 2011, 108, 19755–19760. [Google Scholar] [CrossRef] [PubMed]
  135. Zhang, G.; Raghavan, B.; Kotur, M.; Cheatham, J.; Sedmak, D.; Cook, C.; Waldman, J.; Trgovcich, J. Antisense transcription in the human cytomegalovirus transcriptome. J. Virol. 2007, 81, 11267–11281. [Google Scholar] [CrossRef] [PubMed]
  136. Ma, Y.; Wang, N.; Li, M.; Gao, S.; Wang, L.; Zheng, B.; Qi, Y.; Ruan, Q. Human CMV transcripts: An overview. Future Microbiol. 2012, 7, 577–593. [Google Scholar] [CrossRef] [PubMed]
Figure 1. Schematic of human cytomegalovirus (HCMV) manipulation of translation initiation and elongation. (A) HCMV stimulates eIF4F formation and activity through multiple mechanisms. Infection increases levels of eIF4F components (eIF4E, eIF4G, eIF4A) and poly(A) binding protein (PABP). HCMV also promotes eIF4F assembly by activating mammalian target of rapamycin complex 1 (mTORC1), which phosphorylates and inhibits the eIF4F antagonist 4EBP1. The HCMV UL38 protein prevents inactivation of mTORC1 by inhibiting the tuberous sclerosis complex (TSC). pUL38 also stimulates mTORC1 activity through a TSC2-independent mechanism. mTORC1 activates p70S6K, which phosphorylates eIF4B to increase eIF4A helicase activity. HCMV infection activates the PI3K pathway to promote mTORC1 activation, and may also regulate translation through activation of MNK kinases (B) Translation initiation and elongation are maintained during HCMV infection. Inhibition of eIF2α phosphorylation ensures the regeneration of ternary complexes and continued rounds of translation initiation. Levels of eEF2 increase during infection through a UL38-dependent mechanism and may promote translation during HCMV infection.
Figure 1. Schematic of human cytomegalovirus (HCMV) manipulation of translation initiation and elongation. (A) HCMV stimulates eIF4F formation and activity through multiple mechanisms. Infection increases levels of eIF4F components (eIF4E, eIF4G, eIF4A) and poly(A) binding protein (PABP). HCMV also promotes eIF4F assembly by activating mammalian target of rapamycin complex 1 (mTORC1), which phosphorylates and inhibits the eIF4F antagonist 4EBP1. The HCMV UL38 protein prevents inactivation of mTORC1 by inhibiting the tuberous sclerosis complex (TSC). pUL38 also stimulates mTORC1 activity through a TSC2-independent mechanism. mTORC1 activates p70S6K, which phosphorylates eIF4B to increase eIF4A helicase activity. HCMV infection activates the PI3K pathway to promote mTORC1 activation, and may also regulate translation through activation of MNK kinases (B) Translation initiation and elongation are maintained during HCMV infection. Inhibition of eIF2α phosphorylation ensures the regeneration of ternary complexes and continued rounds of translation initiation. Levels of eEF2 increase during infection through a UL38-dependent mechanism and may promote translation during HCMV infection.
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Vincent, H.A.; Ziehr, B.; Moorman, N.J. Human Cytomegalovirus Strategies to Maintain and Promote mRNA Translation. Viruses 2016, 8, 97. https://doi.org/10.3390/v8040097

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Vincent HA, Ziehr B, Moorman NJ. Human Cytomegalovirus Strategies to Maintain and Promote mRNA Translation. Viruses. 2016; 8(4):97. https://doi.org/10.3390/v8040097

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Vincent, Heather A., Benjamin Ziehr, and Nathaniel J. Moorman. 2016. "Human Cytomegalovirus Strategies to Maintain and Promote mRNA Translation" Viruses 8, no. 4: 97. https://doi.org/10.3390/v8040097

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