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Experimental Inoculation of Egyptian Fruit Bats (Rousettus aegyptiacus) with Ebola Virus

Centre for Emerging and Zoonotic Diseases, National Institute for Communicable Diseases of the National Health Laboratory Service, Sandringham 2131, South Africa
School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa
Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria 0028, South Africa
Author to whom correspondence should be addressed.
Academic Editor: Jens H. Kuhn
Viruses 2016, 8(2), 29;
Received: 27 September 2015 / Revised: 30 December 2015 / Accepted: 6 January 2016 / Published: 22 January 2016
(This article belongs to the Collection Advances in Ebolavirus, Marburgvirus, and Cuevavirus Research)
PDF [644 KB, uploaded 22 January 2016]


Colonized Egyptian fruit bats (Rousettus aegyptiacus), originating in South Africa, were inoculated subcutaneously with Ebola virus (EBOV). No overt signs of morbidity, mortality, or gross lesions were noted. Bats seroconverted by Day 10–16 post inoculation (p.i.), with the highest mean anti-EBOV IgG level on Day 28 p.i. EBOV RNA was detected in blood from one bat. In 16 other tissues tested, viral RNA distribution was limited and at very low levels. No seroconversion could be demonstrated in any of the control bats up to 28 days after in-contact exposure to subcutaneously-inoculated bats. The control bats were subsequently inoculated intraperitoneally, and intramuscularly with the same dose of EBOV. No mortality, morbidity or gross pathology was observed in these bats. Kinetics of immune response was similar to that in subcutaneously-inoculated bats. Viral RNA was more widely disseminated to multiple tissues and detectable in a higher proportion of individuals, but consistently at very low levels. Irrespective of the route of inoculation, no virus was isolated from tissues which tested positive for EBOV RNA. Viral RNA was not detected in oral, nasal, ocular, vaginal, penile and rectal swabs from any of the experimental groups. View Full-Text
Keywords: Egyptian fruit bat; experimental inoculation; Ebola virus; seroconversion; tissue tropism; shedding Egyptian fruit bat; experimental inoculation; Ebola virus; seroconversion; tissue tropism; shedding

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Paweska, J.T.; Storm, N.; Grobbelaar, A.A.; Markotter, W.; Kemp, A.; Jansen van Vuren, P. Experimental Inoculation of Egyptian Fruit Bats (Rousettus aegyptiacus) with Ebola Virus. Viruses 2016, 8, 29.

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