Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains
2.2. Bacterial Culture Conditions
2.3. Bacteriophage Isolation and Propagation
2.4. Host Range, Plaque Morphology and Propagation of ΦPan70
2.5. Genome Size Estimation
2.6. Transmission Electron Microscopy (TEM)
2.7. Activity of ΦPan70 against Planktonic Cells
2.8. Activity of Bacteriophages on Biofilms
2.9. Activity of Bacteriophages in the Burned Mouse Model
2.10. Genome Sequencing, Assembly and Annotation
3. Results
3.1. ΦPan70 is a Member of the Myoviridae Family
3.2. ΦPan70 Reduces Planktonic Cells
3.3. ΦPan70 Reduces Biofilm Formation and Existing Biofilms
3.4. Phage-Therapy with ΦPan70 Improved Survival and Health in the Burned Mouse Model
3.5. Genome Sequencing, Assembly and Annotation
ORF | Function | |
gp001 | Small Subunit DNA Packaging Dimer (Pseudomonas Phage F10) | |
gp002 | Large Subunit DNA Packaging Dimer (Pseudomonas Phage F10) | |
gp004 | Portal Protein (Pseudomonas Phage F10) | |
gp005 * | Putative ClpP Protease (Pseudomonas Phage F10) | |
gp013 * | Putative Tail Length Tape Measure Protein (P. aeruginosa LESB58) | |
gp017 | Tail Fiber Protein (P. aeruginosa PA21_ST175) | |
gp028 | DNA Modification Protein (Desulfovibrio vulgaris str. 'Miyazaki F') | |
gp029 | Site Specific Recombinase (P. aeruginosa ATCC 700888) | |
gp043 | LuxR Family Transcriptional Regulator (P. aeruginosa LESB58) | |
gp044 | Transcriptional Regulator (P. aeruginosa ATCC 25324) | |
gp045 | Repressor (Pseudomonas syringae) | |
gp052 | Rha Family Phage Regulatory Protein (P. aeruginosa LESB58) | |
gp055 | DNA Replication protein, DnaC (P. aeruginosa LESB58) | |
gp056 | Putative DnaB-like Replicative Helicase (P. aeruginosa LESB58) | |
gp058 | Putative Metallophosphoesterase (Pseudomonas PhageF10) | |
gp061 | Holin (P. aeruginosa LESB58) | |
gp62 | Chitinase (P. aeruginosa LESB58) | |
4. Discussion
Supplementary Files
Supplementary File 1Acknowledgments
Author Contributions
Conflicts of Interest
References
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Holguín, A.V.; Rangel, G.; Clavijo, V.; Prada, C.; Mantilla, M.; Gomez, M.C.; Kutter, E.; Taylor, C.; Fineran, P.C.; Barrios, A.F.G.; et al. Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays. Viruses 2015, 7, 4602-4623. https://doi.org/10.3390/v7082835
Holguín AV, Rangel G, Clavijo V, Prada C, Mantilla M, Gomez MC, Kutter E, Taylor C, Fineran PC, Barrios AFG, et al. Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays. Viruses. 2015; 7(8):4602-4623. https://doi.org/10.3390/v7082835
Chicago/Turabian StyleHolguín, Angela V., Guillermo Rangel, Viviana Clavijo, Catalina Prada, Marcela Mantilla, María Catalina Gomez, Elizabeth Kutter, Corinda Taylor, Peter C. Fineran, Andrés Fernando González Barrios, and et al. 2015. "Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays" Viruses 7, no. 8: 4602-4623. https://doi.org/10.3390/v7082835