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Open AccessArticle

The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain

1
Institute for Clinical and Molecular Virology, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
2
Division of Bioinformatics, Institute of Biochemistry, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
3
Lead Discovery Center GmbH, 44227 Dortmund, Germany
4
Department of Histology and Embryology, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Viruses 2015, 7(8), 4582-4601; https://doi.org/10.3390/v7082834
Received: 11 April 2015 / Revised: 12 July 2015 / Accepted: 27 July 2015 / Published: 11 August 2015
(This article belongs to the Section Animal Viruses)
Replication of human cytomegalovirus (HCMV) is characterized by a tight virus-host cell interaction. Cyclin-dependent protein kinases (CDKs) are functionally integrated into viral gene expression and protein modification. The HCMV-encoded protein kinase pUL97 acts as a CDK ortholog showing structural and functional similarities. Recently, we reported an interaction between pUL97 kinase with a subset of host cyclins, in particular with cyclin T1. Here, we describe an interaction of pUL97 at an even higher affinity with cyclin B1. As a striking feature, the interaction between pUL97 and cyclin B1 proved to be strictly dependent on pUL97 activity, as interaction could be abrogated by treatment with pUL97 inhibitors or by inserting mutations into the conserved kinase domain or the nonconserved C-terminus of pUL97, both producing loss of activity. Thus, we postulate that the mechanism of pUL97-cyclin B1 interaction is determined by an active pUL97 kinase domain. View Full-Text
Keywords: human cytomegalovirus; protein kinase pUL97; CDK ortholog; cyclin B; protein-protein interaction; kinase activity; mode of kinase-cyclin interaction; active conformation human cytomegalovirus; protein kinase pUL97; CDK ortholog; cyclin B; protein-protein interaction; kinase activity; mode of kinase-cyclin interaction; active conformation
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Steingruber, M.; Socher, E.; Hutterer, C.; Webel, R.; Bergbrede, T.; Lenac, T.; Sticht, H.; Marschall, M. The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain. Viruses 2015, 7, 4582-4601.

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