The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plasmid Constructs
2.2. Cell Culture, HCMV Infections and Plasmid Transfection
2.3. Antibodies
2.4. Coimmunoprecipitation (CoIP)
2.5. Confocal Imaging
2.6. In Vitro Kinase Assay (IVKA)
2.7. Homology Modeling of pUL97
3. Results
3.1. HCMV Protein Kinase pUL97 Shows a Particularly Strong Interaction with Cyclin B1
3.2. The pUL97-Cyclin B1 Interaction is Dependent on Kinase Activity
3.3. Serial Deletions of the pUL97 C-Terminus Abrogate Cyclin B1 Interaction
3.4. The Cyclin B1-pUL97 Complex Precipitated from HCMV-Infected Cells Comprises Substrate-Binding and Substrate-Phosphorylating Activity
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References and Notes
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Steingruber, M.; Socher, E.; Hutterer, C.; Webel, R.; Bergbrede, T.; Lenac, T.; Sticht, H.; Marschall, M. The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain. Viruses 2015, 7, 4582-4601. https://doi.org/10.3390/v7082834
Steingruber M, Socher E, Hutterer C, Webel R, Bergbrede T, Lenac T, Sticht H, Marschall M. The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain. Viruses. 2015; 7(8):4582-4601. https://doi.org/10.3390/v7082834
Chicago/Turabian StyleSteingruber, Mirjam, Eileen Socher, Corina Hutterer, Rike Webel, Tim Bergbrede, Tihana Lenac, Heinrich Sticht, and Manfred Marschall. 2015. "The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain" Viruses 7, no. 8: 4582-4601. https://doi.org/10.3390/v7082834
APA StyleSteingruber, M., Socher, E., Hutterer, C., Webel, R., Bergbrede, T., Lenac, T., Sticht, H., & Marschall, M. (2015). The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain. Viruses, 7(8), 4582-4601. https://doi.org/10.3390/v7082834