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Viruses 2015, 7(5), 2378-2403;

Influence of Cellular Trafficking Pathway on Bluetongue Virus Infection in Ovine Cells

Department of Pathogen Molecular Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
Author to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 25 March 2015 / Accepted: 6 May 2015 / Published: 13 May 2015
(This article belongs to the Section Animal Viruses)
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Bluetongue virus (BTV), a non-enveloped arbovirus, causes hemorrhagic disease in ruminants. However, the influence of natural host cell proteins on BTV replication process is not defined. In addition to cell lysis, BTV also exits non-ovine cultured cells by non-lytic pathways mediated by nonstructural protein NS3 that interacts with virus capsid and cellular proteins belonging to calpactin and ESCRT family. The PPXY late domain motif known to recruit NEDD4 family of HECT ubiquitin E3 ligases is also highly conserved in NS3. In this study using a mixture of molecular, biochemical and microscopic techniques we have analyzed the importance of ovine cellular proteins and vesicles in BTV infection. Electron microscopic analysis of BTV infected ovine cells demonstrated close association of mature particles with intracellular vesicles. Inhibition of Multi Vesicular Body (MVB) resident lipid phosphatidylinositol-3-phosphate resulted in decreased total virus titre suggesting that the vesicles might be MVBs. Proteasome mediated inhibition of ubiquitin or modification of virus lacking the PPXY in NS3 reduced virus growth. Thus, our study demonstrated that cellular components comprising of MVB and exocytic pathways proteins are involved in BTV replication in ovine cells. View Full-Text
Keywords: late domain; multivesicular body; ubiquitin; BTV; trafficking; release; NS3 late domain; multivesicular body; ubiquitin; BTV; trafficking; release; NS3

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Bhattacharya, B.; Celma, C.C.; Roy, P. Influence of Cellular Trafficking Pathway on Bluetongue Virus Infection in Ovine Cells. Viruses 2015, 7, 2378-2403.

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