Generation of Recombinant Rabies Virus CVS-11 Expressing eGFP Applied to the Rapid Virus Neutralization Test
Abstract
:1. Introduction
2. Experimental Section
2.1. Viruses and Cells
2.2. Serum Samples
2.3. Construction of the Recombinant rCVS-11-eGFP Strain
2.4. Recovery of the Recombinant rCVS-11-eGFP Strain from Cloned cDNA
2.5. Confirmation of the rCVS-11-eGFP Strain
2.6. Virus Multistep Growth Assay
2.7. Pathogenicity of rCVS-11-eGFP in Mice
2.8. Fluorescent Antibody Virus Neutralization Tests (FAVN)
2.9. The FAVN-eGFP Method
2.10. Data Analyses
3. Results
3.1. Recovery of the rCVS-11-eGFP Strain
3.2. Growth Properties of rCVS-11-eGFP
3.3. Comparative Analysis of VNA Titers Determined by Standard FAVN and FAVN-eGFP Methods
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References and Notes
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Xue, X.; Zheng, X.; Liang, H.; Feng, N.; Zhao, Y.; Gao, Y.; Wang, H.; Yang, S.; Xia, X. Generation of Recombinant Rabies Virus CVS-11 Expressing eGFP Applied to the Rapid Virus Neutralization Test. Viruses 2014, 6, 1578-1589. https://doi.org/10.3390/v6041578
Xue X, Zheng X, Liang H, Feng N, Zhao Y, Gao Y, Wang H, Yang S, Xia X. Generation of Recombinant Rabies Virus CVS-11 Expressing eGFP Applied to the Rapid Virus Neutralization Test. Viruses. 2014; 6(4):1578-1589. https://doi.org/10.3390/v6041578
Chicago/Turabian StyleXue, Xianghong, Xuexing Zheng, Hongru Liang, Na Feng, Yongkun Zhao, Yuwei Gao, Hualei Wang, Songtao Yang, and Xianzhu Xia. 2014. "Generation of Recombinant Rabies Virus CVS-11 Expressing eGFP Applied to the Rapid Virus Neutralization Test" Viruses 6, no. 4: 1578-1589. https://doi.org/10.3390/v6041578