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Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis

Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, CP. 66451, Mexico
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Viruses 2013, 5(3), 792-805; https://doi.org/10.3390/v5030792
Received: 13 February 2013 / Revised: 4 March 2013 / Accepted: 5 March 2013 / Published: 11 March 2013
The NSP4 protein is a multifunctional protein that plays a role in the morphogenesis and pathogenesis of the rotavirus. Although NSP4 is considered an enterotoxin, the relationship between gastroenteritis severity and amino acid variations in NSP4 of the human rotavirus remains unclear. In this study, we analyzed the sequence diversity of NSP4 and the severity of gastroenteritis of children with moderate to severe gastroenteritis. The rotavirus-infected children were hospitalized before the rotavirus vaccine program in Mexico. All children had diarrhea within 1-4 days, 44 (88%) were vomiting and 35 (70%) had fevers. The severity analysis showed that 13 (26%) cases had mild gastroenteritis, 23 (46%) moderate gastroenteritis and 14 (28%) severe. NSP4 phylogenetic analysis showed three clusters within the genotype E1. Sequence analysis revealed similar mutations inside each cluster, and an uncommon variation in residue 144 was found in five of the Mexican NSP4 sequences. Most of the amino acid variations were located in the VP4 and VP6 binding site domains, with no relationship to different grades of gastroenteritis. This finding indicates that severe gastroenteritis caused by the rotavirus appears to be related to diverse viral or cellular factors instead of NSP4 activity as a unique pathogenic factor. View Full-Text
Keywords: rotavirus; severe gastroenteritis; NSP4 rotavirus; severe gastroenteritis; NSP4
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MDPI and ACS Style

González-Ochoa, G.; Menchaca, G.E.; Hernández, C.E.; Rodríguez, C.; Tamez, R.S.; Contreras, J.F. Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis. Viruses 2013, 5, 792-805. https://doi.org/10.3390/v5030792

AMA Style

González-Ochoa G, Menchaca GE, Hernández CE, Rodríguez C, Tamez RS, Contreras JF. Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis. Viruses. 2013; 5(3):792-805. https://doi.org/10.3390/v5030792

Chicago/Turabian Style

González-Ochoa, Guadalupe, Griselda E. Menchaca, Carlos E. Hernández, Cristina Rodríguez, Reyes S. Tamez, and Juan F. Contreras. 2013. "Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis" Viruses 5, no. 3: 792-805. https://doi.org/10.3390/v5030792

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