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Complete Sequence, Analysis and Organization of the Orgyia leucostigma Nucleopolyhedrovirus Genome

Sylvar Technologies Inc., P.O. Box 636 Station A, Fredericton, New Brunswick, E3B 5A6, Canada
Natural Resources Canada, Atlantic Forestry Centre, Canadian Forest Service, 1350 Regent Street, Fredericton, New Brunswick, E3C 2G6, Canada
Lancaster Environment Centre, Lancaster University, Lancaster, Lancashire, LA1 4YQ, UK
Natural Resources Canada, Great Lakes Forestry Centre, Canadian Forest Service, 1219 Queen Street East, Sault Ste. Marie, Ontario, P6A 2E5, Canada
Author to whom correspondence should be addressed.
Viruses 2011, 3(11), 2301-2327;
Received: 23 August 2011 / Revised: 25 October 2011 / Accepted: 25 October 2011 / Published: 15 November 2011
(This article belongs to the Special Issue Insect Viruses)
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The complete genome of the Orgyia leucostigma nucleopolyhedrovirus (OrleNPV) isolated from the whitemarked tussock moth (Orgyia leucostigma, Lymantridae: Lepidoptera) was sequenced, analyzed, and compared to other baculovirus genomes. The size of the OrleNPV genome was 156,179 base pairs (bp) and had a G+C content of 39%. The genome encoded 135 putative open reading frames (ORFs), which occupied 79% of the entire genome sequence. Three inhibitor of apoptosis (ORFs 16, 43 and 63), and five baculovirus repeated ORFs (bro-a through bro-e) were interspersed in the OrleNPV genome. In addition to six direct repeat (drs), a common feature shared among most baculoviruses, OrleNPV genome contained three homologous regions (hrs) that are located in the latter half of the genome. The presence of an F-protein homologue and the results from phylogenetic analyses placed OrleNPV in the genus Alphabaculovirus, group II. Overall, OrleNPV appears to be most closely related to group II alphabaculoviruses Ectropis obliqua (EcobNPV), Apocheima cinerarium (ApciNPV), Euproctis pseudoconspersa (EupsNPV), and Clanis bilineata (ClbiNPV).
Keywords: alphabaculovirus; baculovirus; white-marked tussock moth alphabaculovirus; baculovirus; white-marked tussock moth

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Thumbi, D.K.; Eveleigh, R.J.M.; Lucarotti, C.J.; Lapointe, R.; Graham, R.I.; Pavlik, L.; Lauzon, H.A.M.; Arif, B.M. Complete Sequence, Analysis and Organization of the Orgyia leucostigma Nucleopolyhedrovirus Genome. Viruses 2011, 3, 2301-2327.

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