Next Article in Journal
Screening of Medicinal Herbs Identifies Cimicifuga foetida and Its Bioactive Component Caffeic Acid as SARS-CoV-2 Entry Inhibitors
Previous Article in Journal
Guinea Pig X Virus Is a Gammaherpesvirus
Previous Article in Special Issue
Evaluation of Humoral and Cell-Mediated Immunity Induced by Quadrivalent Influenza Vaccine in Pre-COVID-19 Japan
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Single-Dose Intranasal or Intramuscular Administration of Simian Adenovirus-Based H1N1 Vaccine Induces a Robust Humoral Response and Complete Protection in Mice

by
Daria V. Voronina
*,†,
Irina V. Vavilova
,
Olga V. Zubkova
,
Tatiana A. Ozharovskaia
,
Olga Popova
,
Anastasia S. Chugunova
,
Polina P. Goldovskaya
,
Denis I. Zrelkin
,
Daria M. Savina
,
Irina A. Favorskaya
,
Dmitry V. Shcheblyakov
,
Denis Y. Logunov
and
Alexandr L. Gintsburg
N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Ministry of Health, Moscow 123098, Russia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2025, 17(8), 1085; https://doi.org/10.3390/v17081085
Submission received: 11 July 2025 / Revised: 3 August 2025 / Accepted: 4 August 2025 / Published: 5 August 2025

Abstract

Despite the widespread accessibility of vaccines and antivirals, seasonal influenza virus epidemics continue to pose a threat to public health. In this study, we constructed a recombinant replication-deficient simian adenovirus type 25 vector carrying the full-length hemagglutinin (HA) of the H1N1 influenza virus, named rSAd25-H1. Both systemic and mucosal humoral immune responses, as well as the protective efficacy, were assessed in mice immunized via the intramuscular (IM) or intranasal (IN) route. A single-dose IM or IN administration of rSAd25-H1 elicited a robust systemic IgG antibody response, including hemagglutination inhibition antibodies. As expected, only IN immunization was able to induce IgA production in serum and respiratory mucosa. Notably, a single dose of rSAd25-H1 at the highest dose (1010 viral particles) conferred complete protection against lethal homologous H1N1 challenge in mice despite the route of administration. These findings demonstrate the potential of simian adenovirus type 25-based vectors as a promising candidate for intranasal vaccine development targeting respiratory pathogens.
Keywords: SAd25; ChAd68; adenoviral vector; influenza; H1N1; humoral immunity SAd25; ChAd68; adenoviral vector; influenza; H1N1; humoral immunity

Share and Cite

MDPI and ACS Style

Voronina, D.V.; Vavilova, I.V.; Zubkova, O.V.; Ozharovskaia, T.A.; Popova, O.; Chugunova, A.S.; Goldovskaya, P.P.; Zrelkin, D.I.; Savina, D.M.; Favorskaya, I.A.; et al. Single-Dose Intranasal or Intramuscular Administration of Simian Adenovirus-Based H1N1 Vaccine Induces a Robust Humoral Response and Complete Protection in Mice. Viruses 2025, 17, 1085. https://doi.org/10.3390/v17081085

AMA Style

Voronina DV, Vavilova IV, Zubkova OV, Ozharovskaia TA, Popova O, Chugunova AS, Goldovskaya PP, Zrelkin DI, Savina DM, Favorskaya IA, et al. Single-Dose Intranasal or Intramuscular Administration of Simian Adenovirus-Based H1N1 Vaccine Induces a Robust Humoral Response and Complete Protection in Mice. Viruses. 2025; 17(8):1085. https://doi.org/10.3390/v17081085

Chicago/Turabian Style

Voronina, Daria V., Irina V. Vavilova, Olga V. Zubkova, Tatiana A. Ozharovskaia, Olga Popova, Anastasia S. Chugunova, Polina P. Goldovskaya, Denis I. Zrelkin, Daria M. Savina, Irina A. Favorskaya, and et al. 2025. "Single-Dose Intranasal or Intramuscular Administration of Simian Adenovirus-Based H1N1 Vaccine Induces a Robust Humoral Response and Complete Protection in Mice" Viruses 17, no. 8: 1085. https://doi.org/10.3390/v17081085

APA Style

Voronina, D. V., Vavilova, I. V., Zubkova, O. V., Ozharovskaia, T. A., Popova, O., Chugunova, A. S., Goldovskaya, P. P., Zrelkin, D. I., Savina, D. M., Favorskaya, I. A., Shcheblyakov, D. V., Logunov, D. Y., & Gintsburg, A. L. (2025). Single-Dose Intranasal or Intramuscular Administration of Simian Adenovirus-Based H1N1 Vaccine Induces a Robust Humoral Response and Complete Protection in Mice. Viruses, 17(8), 1085. https://doi.org/10.3390/v17081085

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Article metric data becomes available approximately 24 hours after publication online.
Back to TopTop