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Volume 16
Issue 6
10.3390/v16060948
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Article

Antiviral Activities of Mastoparan-L-Derived Peptides against Human Alphaherpesvirus 1

by
Liana Costa Pereira Vilas Boas
1,2,
Danieli Fernanda Buccini
2,3,
Rhayfa Lorrayne Araújo Berlanda
1,2,
Bruno de Paula Oliveira Santos
4,
Mariana Rocha Maximiano
2,3,
Luciano Morais Lião
4,
Sónia Gonçalves
5,
Nuno C. Santos
5 and
Octávio Luiz Franco
1,2,3,*
1
Pós-Graduação em Patologia Molecular, Campus Darcy Ribeiro, Universidade de Brasília, Brasília 70910-900, DF, Brazil
2
Centro de Análises Bioquímicas e Proteômicas, Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília 70790-760, DF, Brazil
3
Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande 79117-900, MS, Brazil
4
Laboratório de Ressonância Magnética Nuclear, Instituto de Química, Universidade Federal de Goiás, Goiânia 74690-900, GO, Brazil
5
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal
*
Author to whom correspondence should be addressed.
Viruses 2024, 16(6), 948; https://doi.org/10.3390/v16060948
Submission received: 9 March 2024 / Revised: 1 June 2024 / Accepted: 4 June 2024 / Published: 12 June 2024
(This article belongs to the Special Issue Antiviral Peptide)
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Abstract

Human alphaherpesvirus 1 (HSV-1) is a significantly widespread viral pathogen causing recurrent infections that are currently incurable despite available treatment protocols. Studies have highlighted the potential of antimicrobial peptides sourced from Vespula lewisii venom, particularly those belonging to the mastoparan family, as effective against HSV-1. This study aimed to demonstrate the antiviral properties of mastoparans, including mastoparan-L [I5, R8], mastoparan-MO, and [I5, R8] mastoparan, against HSV-1. Initially, Vero cell viability was assessed in the presence of these peptides, followed by the determination of antiviral activity, mechanism of action, and dose-response curves through plaque assays. Structural analyses via circular dichroism and nuclear magnetic resonance were conducted, along with evaluating membrane fluidity changes induced by [I5, R8] mastoparan using fluorescence-labeled lipid vesicles. Cytotoxic assays revealed high cell viability (>80%) at concentrations of 200 µg/mL for mastoparan-L and mastoparan-MO and 50 µg/mL for [I5, R8] mastoparan. Mastoparan-MO and [I5, R8] mastoparan exhibited over 80% HSV-1 inhibition, with up to 99% viral replication inhibition, particularly in the early infection stages. Structural analysis indicated an α-helical structure for [I5, R8] mastoparan, suggesting effective viral particle disruption before cell attachment. Mastoparans present promising prospects for HSV-1 infection control, although further investigation into their mechanisms is warranted.
Keywords: antiviral peptide; mastoparan; peptide–membrane interaction antiviral peptide; mastoparan; peptide–membrane interaction

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MDPI and ACS Style

Vilas Boas, L.C.P.; Buccini, D.F.; Berlanda, R.L.A.; Santos, B.d.P.O.; Maximiano, M.R.; Lião, L.M.; Gonçalves, S.; Santos, N.C.; Franco, O.L. Antiviral Activities of Mastoparan-L-Derived Peptides against Human Alphaherpesvirus 1. Viruses 2024, 16, 948. https://doi.org/10.3390/v16060948

AMA Style

Vilas Boas LCP, Buccini DF, Berlanda RLA, Santos BdPO, Maximiano MR, Lião LM, Gonçalves S, Santos NC, Franco OL. Antiviral Activities of Mastoparan-L-Derived Peptides against Human Alphaherpesvirus 1. Viruses. 2024; 16(6):948. https://doi.org/10.3390/v16060948

Chicago/Turabian Style

Vilas Boas, Liana Costa Pereira, Danieli Fernanda Buccini, Rhayfa Lorrayne Araújo Berlanda, Bruno de Paula Oliveira Santos, Mariana Rocha Maximiano, Luciano Morais Lião, Sónia Gonçalves, Nuno C. Santos, and Octávio Luiz Franco. 2024. "Antiviral Activities of Mastoparan-L-Derived Peptides against Human Alphaherpesvirus 1" Viruses 16, no. 6: 948. https://doi.org/10.3390/v16060948

APA Style

Vilas Boas, L. C. P., Buccini, D. F., Berlanda, R. L. A., Santos, B. d. P. O., Maximiano, M. R., Lião, L. M., Gonçalves, S., Santos, N. C., & Franco, O. L. (2024). Antiviral Activities of Mastoparan-L-Derived Peptides against Human Alphaherpesvirus 1. Viruses, 16(6), 948. https://doi.org/10.3390/v16060948

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