Viral Interference between the Insect-Specific Virus Brejeira and the Saint Louis Encephalitis Virus In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Viral Samples
2.2. Cells
2.3. Preparation and Titration of the Viral Stock
2.4. Tissue Culture Infectious Dose 50% (TCID50)
2.5. Co-Infection Experiments in Cell Culture between SLEV and BREV
2.6. Indirect Immunofluorescence (IFA) and Viral Titration
2.7. Real-Time Reverse Transcription–Polymerase Chain Reaction (Real-Time RT-PCR) for SLEV and BREV
2.8. Statistical Analysis
3. Results
3.1. Viral Titers
3.2. SLEV Co-Infection and BREV in C6/36 Cells
3.2.1. Observing the Cytopathic Effect (CPE)
3.2.2. Detection of SLEV Viral Antigens by IFA
3.2.3. SLEV Titer Curve
3.2.4. Viral Genome Detection for SLEV and BREV
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Virus | Primers/Probes |
---|---|
SLEV | SLE2420 2420–2439 CTGGCTGTCGGAGGGATTCT 68 (Primer) |
SLE2487c 2487–2468 TAGGTCAATTGCACATCCCG (Primer) | |
SLE2444-probe 2444–2466 TCTGGCGACCAGCGTGCAAGCCG (Probe) | |
SLE834 834–852 GAAAACTGGGTTCTGCGCA 72 (Primer) | |
SLE905c 905–889 GTTGCTGCCTAGCATCCATCC (Primer) | |
SLE857-probe 857–880 TGGATATGCCCTAGTTGCGCTGGC (Probe) | |
BREV | BREV–F–ATGACCGATGATGAGAACCG (Primer) |
BREV–R–GGTGAGACAGCAATAGTAGCC (Primer) | |
BREV–Probe–TCGTGCTCGATGACACCCGC (Probe) |
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Ribeiro, A.C.; Martins, L.; Silva, H.; Freitas, M.N.; Santos, M.; Gonçalves, E.; Sousa, A.; Prazeres, I.; Santos, A.; Cruz, A.C.; et al. Viral Interference between the Insect-Specific Virus Brejeira and the Saint Louis Encephalitis Virus In Vitro. Viruses 2024, 16, 210. https://doi.org/10.3390/v16020210
Ribeiro AC, Martins L, Silva H, Freitas MN, Santos M, Gonçalves E, Sousa A, Prazeres I, Santos A, Cruz AC, et al. Viral Interference between the Insect-Specific Virus Brejeira and the Saint Louis Encephalitis Virus In Vitro. Viruses. 2024; 16(2):210. https://doi.org/10.3390/v16020210
Chicago/Turabian StyleRibeiro, Ana Cláudia, Lívia Martins, Heloisa Silva, Maria Nazaré Freitas, Maissa Santos, Ercília Gonçalves, Alana Sousa, Ivy Prazeres, Alessandra Santos, Ana Cecilia Cruz, and et al. 2024. "Viral Interference between the Insect-Specific Virus Brejeira and the Saint Louis Encephalitis Virus In Vitro" Viruses 16, no. 2: 210. https://doi.org/10.3390/v16020210
APA StyleRibeiro, A. C., Martins, L., Silva, H., Freitas, M. N., Santos, M., Gonçalves, E., Sousa, A., Prazeres, I., Santos, A., Cruz, A. C., Silva, S., Chiang, J., Casseb, L., & Carvalho, V. (2024). Viral Interference between the Insect-Specific Virus Brejeira and the Saint Louis Encephalitis Virus In Vitro. Viruses, 16(2), 210. https://doi.org/10.3390/v16020210