Host Responses to Live-Attenuated ASFV (HLJ/18–7GD)

Fan, Yuqin; Chen, Weiye; Jiang, Chenggang; Zhang, Xianfeng; Sun, Ying; Liu, Renqiang; Wang, Jingfei; Yang, Decheng; Zhao, Dongming; Bu, Zhigao; He, Xijun

doi:10.3390/v14092003

Open AccessArticle

Host Responses to Live-Attenuated ASFV (HLJ/18–7GD)

by

Yuqin Fan

^†,

Weiye Chen

^†,

Chenggang Jiang

,

Xianfeng Zhang

,

Ying Sun

,

Renqiang Liu

,

Jingfei Wang

,

Decheng Yang

,

Dongming Zhao

^*

,

Zhigao Bu

^* and

Xijun He

^*

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Viruses 2022, 14(9), 2003; https://doi.org/10.3390/v14092003

Submission received: 6 July 2022 / Revised: 6 September 2022 / Accepted: 8 September 2022 / Published: 10 September 2022

(This article belongs to the Special Issue African Swine Fever Virus 2.0)

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Abstract

African swine fever (ASF) is a highly contagious and fatal disease caused by the African swine fever virus. Recently, the multigene family and CD2v gene-deleted ASF vaccine candidate HLJ/18-7GD was found to be safe and effective in laboratory and clinical trials. However, the immune-protective mechanisms underlying the effects of HLJ/18-7GD remain unclear. We assessed samples from pigs immunized with a single dose of 10⁶ TCID₅₀ HLJ/18-7GD. We found that pigs immunized with HLJ/18-7GD showed high levels of specific antibodies. T lymphocyte subsets (helper T cells (Th); cytotoxic T lymphocytes (CTL); double-positive T cells (DP-T cells)) were temporarily increased in peripheral blood mononuclear cells (PBMCs) after HLJ/18-7GD immunization. Once the HLJ/18-7GD-immunized pigs had been challenged with virulent HLJ/18, the percentage of Th, CTL, and DP-T cells increased significantly. PBMCs extracted from the pigs induced higher levels of CD8⁺ T cells after infection with the HLJ/18 strain in vitro. The levels of GM-CSF, IFN-γ, and TNF-α were upregulated at 7 days post-inoculation; this finding was contrary to the results obtained after HLJ/18 or HLJ/18ΔCD2v infection. The immune protection from HLJ/18-7GD resulted from many synergies, which could provide a theoretical basis for HLJ/18-7GD as a safe and effective ASF vaccine.

Keywords: HLJ/18-7GD strain; ASF; antibody; cytokine; T cell

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MDPI and ACS Style

Fan, Y.; Chen, W.; Jiang, C.; Zhang, X.; Sun, Y.; Liu, R.; Wang, J.; Yang, D.; Zhao, D.; Bu, Z.; et al. Host Responses to Live-Attenuated ASFV (HLJ/18–7GD). Viruses 2022, 14, 2003. https://doi.org/10.3390/v14092003

AMA Style

Fan Y, Chen W, Jiang C, Zhang X, Sun Y, Liu R, Wang J, Yang D, Zhao D, Bu Z, et al. Host Responses to Live-Attenuated ASFV (HLJ/18–7GD). Viruses. 2022; 14(9):2003. https://doi.org/10.3390/v14092003

Chicago/Turabian Style

Fan, Yuqin, Weiye Chen, Chenggang Jiang, Xianfeng Zhang, Ying Sun, Renqiang Liu, Jingfei Wang, Decheng Yang, Dongming Zhao, Zhigao Bu, and et al. 2022. "Host Responses to Live-Attenuated ASFV (HLJ/18–7GD)" Viruses 14, no. 9: 2003. https://doi.org/10.3390/v14092003

APA Style

Fan, Y., Chen, W., Jiang, C., Zhang, X., Sun, Y., Liu, R., Wang, J., Yang, D., Zhao, D., Bu, Z., & He, X. (2022). Host Responses to Live-Attenuated ASFV (HLJ/18–7GD). Viruses, 14(9), 2003. https://doi.org/10.3390/v14092003

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Host Responses to Live-Attenuated ASFV (HLJ/18–7GD)

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