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Article

Expanding Mouse-Adapted Yamagata-like Influenza B Viruses in Eggs Enhances In Vivo Lethality in BALB/c Mice

1
Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
2
Department of Infectious Diseases, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Llilianne Ganges and Jishu Shi
Viruses 2022, 14(6), 1299; https://doi.org/10.3390/v14061299
Received: 23 May 2022 / Accepted: 10 June 2022 / Published: 14 June 2022
Despite the yearly global impact of influenza B viruses (IBVs), limited host range has been a hurdle to developing a readily accessible small animal disease model for vaccine studies. Mouse-adapting IBV can produce highly pathogenic viruses through serial lung passaging in mice. Previous studies have highlighted amino acid changes throughout the viral genome correlating with increased pathogenicity, but no consensus mutations have been determined. We aimed to show that growth system can play a role in mouse-adapted IBV lethality. Two Yamagata-lineage IBVs were serially passaged 10 times in mouse lungs before expansion in embryonated eggs or Madin–Darby canine kidney cells (London line) for use in challenge studies. We observed that virus grown in embryonated eggs was significantly more lethal in mice than the same virus grown in cell culture. Ten additional serial lung passages of one strain again showed virus grown in eggs was more lethal than virus grown in cells. Additionally, no mutations in the surface glycoprotein amino acid sequences correlated to differences in lethality. Our results suggest growth system can influence lethality of mouse-adapted IBVs after serial lung passaging. Further research can highlight improved mechanisms for developing animal disease models for IBV vaccine research. View Full-Text
Keywords: influenza B virus; mouse-adapting; growth system; embryonated eggs; hemagglutinin; neuraminidase influenza B virus; mouse-adapting; growth system; embryonated eggs; hemagglutinin; neuraminidase
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MDPI and ACS Style

Pekarek, M.J.; Petro-Turnquist, E.M.; Rubrum, A.; Webby, R.J.; Weaver, E.A. Expanding Mouse-Adapted Yamagata-like Influenza B Viruses in Eggs Enhances In Vivo Lethality in BALB/c Mice. Viruses 2022, 14, 1299. https://doi.org/10.3390/v14061299

AMA Style

Pekarek MJ, Petro-Turnquist EM, Rubrum A, Webby RJ, Weaver EA. Expanding Mouse-Adapted Yamagata-like Influenza B Viruses in Eggs Enhances In Vivo Lethality in BALB/c Mice. Viruses. 2022; 14(6):1299. https://doi.org/10.3390/v14061299

Chicago/Turabian Style

Pekarek, Matthew J., Erika M. Petro-Turnquist, Adam Rubrum, Richard J. Webby, and Eric A. Weaver. 2022. "Expanding Mouse-Adapted Yamagata-like Influenza B Viruses in Eggs Enhances In Vivo Lethality in BALB/c Mice" Viruses 14, no. 6: 1299. https://doi.org/10.3390/v14061299

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