Systemic Neutralizing Antibodies and Local Immune Responses Are Critical for the Control of SARS-CoV-2
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Preparation of SARS-CoV-2 Vaccine Constructs
2.3. Characterization of Nanoparticles
2.4. J774 Uptake Experiment
2.5. Vaccine Efficacy Study
2.6. SARS-CoV-2 Neutralization Assay
2.7. Flow Cytometric Assessment of SARS-CoV-2 Specific Intracellular Cytokine Assay
2.8. Viral Load Measurement
2.9. Histopathological Analysis
2.10. Statistical Analysis
3. Results
3.1. Characterization of Nanoparticles Formed by QAC Encapsulation of Plasmid DNA Immunogens
3.2. Internalization of Plasmid DNA by Macrophages When Complexed with QAC Adjuvant System
3.3. Systemic SARS-CoV-2 Specific Immune Responses in Vaccinated Mice
3.4. Intranasal Administration of SARS-CoV-2 Vaccine Induces Lung Cellular Responses
3.5. QAC Based Immunizations Reduce Viral Burden in Transgenic Mice
3.6. Reduced Viral Pneumonia and Tissue Damage in Vaccinated Transgenic Mice
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chandrasekar, S.S.; Phanse, Y.; Riel, M.; Hildebrand, R.E.; Hanafy, M.; Osorio, J.E.; Abdelgayed, S.S.; Talaat, A.M. Systemic Neutralizing Antibodies and Local Immune Responses Are Critical for the Control of SARS-CoV-2. Viruses 2022, 14, 1262. https://doi.org/10.3390/v14061262
Chandrasekar SS, Phanse Y, Riel M, Hildebrand RE, Hanafy M, Osorio JE, Abdelgayed SS, Talaat AM. Systemic Neutralizing Antibodies and Local Immune Responses Are Critical for the Control of SARS-CoV-2. Viruses. 2022; 14(6):1262. https://doi.org/10.3390/v14061262
Chicago/Turabian StyleChandrasekar, Shaswath S., Yashdeep Phanse, Mariah Riel, Rachel E. Hildebrand, Mostafa Hanafy, Jorge E. Osorio, Sherein S. Abdelgayed, and Adel M. Talaat. 2022. "Systemic Neutralizing Antibodies and Local Immune Responses Are Critical for the Control of SARS-CoV-2" Viruses 14, no. 6: 1262. https://doi.org/10.3390/v14061262
APA StyleChandrasekar, S. S., Phanse, Y., Riel, M., Hildebrand, R. E., Hanafy, M., Osorio, J. E., Abdelgayed, S. S., & Talaat, A. M. (2022). Systemic Neutralizing Antibodies and Local Immune Responses Are Critical for the Control of SARS-CoV-2. Viruses, 14(6), 1262. https://doi.org/10.3390/v14061262