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Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to Stenotrophomonas maltophilia Clinical Ocular Isolates

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School of Biotechnology and Biomolecular Sciences, The University of New South Wales, High Street, Kensington, NSW 2052, Australia
2
NSW Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, The University of New South Wales, High Street, Kensington, NSW 2052, Australia
3
AusPhage, 21 Everett Street, Mount St John, QLD 4818, Australia
4
School of Optometry and Vision Science, The University of New South Wales, High Street, Kensington, NSW 2052, Australia
*
Authors to whom correspondence should be addressed.
Academic Editor: Dann Turner
Viruses 2022, 14(4), 709; https://doi.org/10.3390/v14040709
Received: 10 February 2022 / Revised: 18 March 2022 / Accepted: 24 March 2022 / Published: 29 March 2022
(This article belongs to the Section Bacterial Viruses)
Recent acknowledgment that multidrug resistant Stenotrophomonas maltophilia strains can cause severe infections has led to increasing global interest in addressing its pathogenicity. While being primarily associated with hospital-acquired respiratory tract infections, this bacterial species is also relevant to ophthalmology, particularly to contact lens-related diseases. In the current study, the capacity of Stenotrophomonas phage vB_SmaM_Ps15 to infect ocular S. maltophilia strains was investigated to explore its future potential as a phage therapeutic. The phage proved to be lytic to a range of clinical isolates collected in Australia from eye swabs, contact lenses and contact lens cases that had previously shown to be resistant to several antibiotics and multipurpose contact lenses disinfectant solutions. Morphological analysis by transmission electron microscopy placed the phage into the Myoviridae family. Its genome size was 161,350 bp with a G + C content of 54.2%, containing 276 putative protein-encoding genes and 24 tRNAs. A detailed comparative genomic analysis positioned vB_SmaM_Ps15 as a new species of the Menderavirus genus, which currently contains six very similar globally distributed members. It was confirmed as a virulent phage, free of known lysogenic and pathogenicity determinants, which supports its potential use for the treatment of S. maltophilia eye infections. View Full-Text
Keywords: Stenotrophomonas maltophilia; bacteriophage; Menderavirus Stenotrophomonas maltophilia; bacteriophage; Menderavirus
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MDPI and ACS Style

Damnjanović, D.; Vázquez-Campos, X.; Elliott, L.; Willcox, M.; Bridge, W.J. Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to Stenotrophomonas maltophilia Clinical Ocular Isolates. Viruses 2022, 14, 709. https://doi.org/10.3390/v14040709

AMA Style

Damnjanović D, Vázquez-Campos X, Elliott L, Willcox M, Bridge WJ. Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to Stenotrophomonas maltophilia Clinical Ocular Isolates. Viruses. 2022; 14(4):709. https://doi.org/10.3390/v14040709

Chicago/Turabian Style

Damnjanović, Dragica, Xabier Vázquez-Campos, Lisa Elliott, Mark Willcox, and Wallace J. Bridge. 2022. "Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to Stenotrophomonas maltophilia Clinical Ocular Isolates" Viruses 14, no. 4: 709. https://doi.org/10.3390/v14040709

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