Sequences Encoding a Novel Toursvirus Identified from Southern and Northern Corn Rootworms (Coleoptera: Chrysomelidae)

Liu, Sijun; Sappington, Thomas W.; Coates, Brad S.; Bonning, Bryony C.

doi:10.3390/v14020397

Open AccessArticle

Sequences Encoding a Novel Toursvirus Identified from Southern and Northern Corn Rootworms (Coleoptera: Chrysomelidae)

¹

Department of Entomology, Iowa State University, Ames, IA 50011, USA

²

Corn Insects and Crop Genetics Research Unit, USDA-ARS, Ames, IA 50011, USA

³

Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611, USA

^*

Author to whom correspondence should be addressed.

Viruses 2022, 14(2), 397; https://doi.org/10.3390/v14020397

Submission received: 1 February 2022 / Accepted: 7 February 2022 / Published: 15 February 2022

(This article belongs to the Section Invertebrate Viruses)

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Abstract

Sequences derived from a novel toursvirus were identified from pooled genomic short read data from U.S. populations of southern corn rootworm (SCR, Diabrotica undecimpunctata howardi Barber) and northern corn rootworm (NCR, Diabrotica barberi Smith & Lawrence). Most viral sequences were identified from the SCR genomic dataset. As proteins encoded by toursvirus sequences from SCR and NCR were almost identical, the contig sets from SCR and NCR were combined to generate 26 contigs. A total of 108,176 bp were assembled from these contigs, with 120 putative toursviral ORFs identified indicating that most of the viral genome had been recovered. These ORFs included all 40 genes that are common to members of the Ascoviridae. Two genes typically present in Ascoviridae (ATP binding cassette transport system permeases and Baculovirus repeated open reading frame), were not detected. There was evidence for transposon insertion in viral sequences at different sites in the two host species. Phylogenetic analyses based on a concatenated set of 45 translated protein sequences clustered toursviruses into a distinct clade. Based on the combined evidence, we propose taxonomic separation of toursviruses from Ascoviridae.

Keywords: Ascoviridae; toursvirust; beetle; corn rootworm; Diabrotica spp.

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MDPI and ACS Style

Liu, S.; Sappington, T.W.; Coates, B.S.; Bonning, B.C. Sequences Encoding a Novel Toursvirus Identified from Southern and Northern Corn Rootworms (Coleoptera: Chrysomelidae). Viruses 2022, 14, 397. https://doi.org/10.3390/v14020397

AMA Style

Liu S, Sappington TW, Coates BS, Bonning BC. Sequences Encoding a Novel Toursvirus Identified from Southern and Northern Corn Rootworms (Coleoptera: Chrysomelidae). Viruses. 2022; 14(2):397. https://doi.org/10.3390/v14020397

Chicago/Turabian Style

Liu, Sijun, Thomas W. Sappington, Brad S. Coates, and Bryony C. Bonning. 2022. "Sequences Encoding a Novel Toursvirus Identified from Southern and Northern Corn Rootworms (Coleoptera: Chrysomelidae)" Viruses 14, no. 2: 397. https://doi.org/10.3390/v14020397

APA Style

Liu, S., Sappington, T. W., Coates, B. S., & Bonning, B. C. (2022). Sequences Encoding a Novel Toursvirus Identified from Southern and Northern Corn Rootworms (Coleoptera: Chrysomelidae). Viruses, 14(2), 397. https://doi.org/10.3390/v14020397

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Sequences Encoding a Novel Toursvirus Identified from Southern and Northern Corn Rootworms (Coleoptera: Chrysomelidae)

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