ADAM17 Is an Essential Factor for the Infection of Bovine Cells with Pestiviruses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. RNAseq Analysis of MDBK and CRIB-1 Cells and Validation
2.3. Cloning of ADAM17 cDNA from MDBK and CRIB-1 Cells and Expression of ADAM17 from MDBK Cells in CRIB-1 Cells
2.4. Genetic Characterization of ADAM17
2.5. Detection of ADAM17 Expression
2.6. Determination of Susceptibility
2.7. Determination of Spread
3. Results
3.1. ADAM17 Alleles Are Disrupted in CRIB-1 Cells
3.2. ADAM17 Transcomplementation Reverts the Phenotype of CRIB-1 Cells for a Diverse Array of Pestiviruses
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zaruba, M.; Chen, H.-W.; Pietsch, O.F.; Szakmary-Braendle, K.; Auer, A.; Mötz, M.; Seitz, K.; Düsterhöft, S.; Workman, A.M.; Rümenapf, T.; et al. ADAM17 Is an Essential Factor for the Infection of Bovine Cells with Pestiviruses. Viruses 2022, 14, 381. https://doi.org/10.3390/v14020381
Zaruba M, Chen H-W, Pietsch OF, Szakmary-Braendle K, Auer A, Mötz M, Seitz K, Düsterhöft S, Workman AM, Rümenapf T, et al. ADAM17 Is an Essential Factor for the Infection of Bovine Cells with Pestiviruses. Viruses. 2022; 14(2):381. https://doi.org/10.3390/v14020381
Chicago/Turabian StyleZaruba, Marianne, Hann-Wei Chen, Ole Frithjof Pietsch, Kati Szakmary-Braendle, Angelika Auer, Marlene Mötz, Kerstin Seitz, Stefan Düsterhöft, Aspen M. Workman, Till Rümenapf, and et al. 2022. "ADAM17 Is an Essential Factor for the Infection of Bovine Cells with Pestiviruses" Viruses 14, no. 2: 381. https://doi.org/10.3390/v14020381