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Communication

Discriminating between JCPyV and BKPyV in Urinary Virome Data Sets

1
Bioinformatics Program, Loyola University Chicago, Chicago, IL 60660, USA
2
Department of Microbiology and Immunology, Stitch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA
3
Department of Biology, Loyola University Chicago, Chicago, IL 60660, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Jennifer R. Brum and Simon Roux
Viruses 2021, 13(6), 1041; https://doi.org/10.3390/v13061041
Received: 11 April 2021 / Revised: 15 May 2021 / Accepted: 27 May 2021 / Published: 31 May 2021
(This article belongs to the Special Issue Viromics)
Polyomaviruses are abundant in the human body. The polyomaviruses JC virus (JCPyV) and BK virus (BKPyV) are common viruses in the human urinary tract. Prior studies have estimated that JCPyV infects between 20 and 80% of adults and that BKPyV infects between 65 and 90% of individuals by age 10. However, these two viruses encode for the same six genes and share 75% nucleotide sequence identity across their genomes. While prior urinary virome studies have repeatedly reported the presence of JCPyV, we were interested in seeing how JCPyV prevalence compares to BKPyV. We retrieved all publicly available shotgun metagenomic sequencing reads from urinary microbiome and virome studies (n = 165). While one third of the data sets produced hits to JCPyV, upon further investigation were we able to determine that the majority of these were in fact BKPyV. This distinction was made by specifically mining for JCPyV and BKPyV and considering uniform coverage across the genome. This approach provides confidence in taxon calls, even between closely related viruses with significant sequence similarity. View Full-Text
Keywords: JCPyV; BKPyV; polyomaviruses; urinary virome; urinary microbiome JCPyV; BKPyV; polyomaviruses; urinary virome; urinary microbiome
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MDPI and ACS Style

Mormando, R.; Wolfe, A.J.; Putonti, C. Discriminating between JCPyV and BKPyV in Urinary Virome Data Sets. Viruses 2021, 13, 1041. https://doi.org/10.3390/v13061041

AMA Style

Mormando R, Wolfe AJ, Putonti C. Discriminating between JCPyV and BKPyV in Urinary Virome Data Sets. Viruses. 2021; 13(6):1041. https://doi.org/10.3390/v13061041

Chicago/Turabian Style

Mormando, Rita, Alan J. Wolfe, and Catherine Putonti. 2021. "Discriminating between JCPyV and BKPyV in Urinary Virome Data Sets" Viruses 13, no. 6: 1041. https://doi.org/10.3390/v13061041

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