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Article

Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations

1
Kuopio Center for Gene and Cell Therapy, 70210 Kuopio, Finland
2
Molecular Medicine Research Group, A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 70210 Kuopio, Finland
3
Heart Center and Gene Therapy Unit, Kuopio University Hospital, 70210 Kuopio, Finland
*
Author to whom correspondence should be addressed.
Academic Editor: Stefan Weger
Viruses 2021, 13(5), 939; https://doi.org/10.3390/v13050939
Received: 28 March 2021 / Revised: 14 May 2021 / Accepted: 17 May 2021 / Published: 19 May 2021
(This article belongs to the Special Issue Novel Developments and Perspectives in Viral Vector Technology)
Virus particle concentration is a critical piece of information for virology, viral vaccines and gene therapy research. We tested a novel nanoparticle counting device, “Videodrop”, for its efficacy in titering and characterization of virus particles. The Videodrop nanoparticle counter is based on interferometric light microscopy (ILM). The method allows the detection of particles under the diffraction limit capabilities of conventional light microscopy. We analyzed lenti-, adeno-, and baculovirus samples in different concentrations and compared the readings against traditional titering and characterization methods. The tested Videodrop particle counter is especially useful when measuring high-concentration purified virus preparations. Certain non-purified sample types or small viruses may be impossible to characterize or may require the use of standard curve or background subtraction methods, which increases the duration of the analysis. Together, our testing shows that Videodrop is a reasonable option for virus particle counting in situations where a moderate number of samples need to be analyzed quickly. View Full-Text
Keywords: automated nanoparticle counter; titering; viruses; virus vectors; interferometric light microscopy; particle count automated nanoparticle counter; titering; viruses; virus vectors; interferometric light microscopy; particle count
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MDPI and ACS Style

Turkki, V.; Alppila, E.; Ylä-Herttuala, S.; Lesch, H.P. Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations. Viruses 2021, 13, 939. https://doi.org/10.3390/v13050939

AMA Style

Turkki V, Alppila E, Ylä-Herttuala S, Lesch HP. Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations. Viruses. 2021; 13(5):939. https://doi.org/10.3390/v13050939

Chicago/Turabian Style

Turkki, Vesa, Elisa Alppila, Seppo Ylä-Herttuala, and Hanna P. Lesch 2021. "Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations" Viruses 13, no. 5: 939. https://doi.org/10.3390/v13050939

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