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Article

An Early Block in the Replication of the Atypical Bluetongue Virus Serotype 26 in Culicoides Cells Is Determined by Its Capsid Proteins

1
The Pirbright Institute, Pirbright, Surrey GU24 0NF, UK
2
Institute of Infection and Global Health, University of Liverpool, Liverpool Science Park IC2, Liverpool L3 5RF, UK
3
MRC-University of Glasgow Centre for Virus Research, Sir Michael Stoker Building, Garscube Campus, University of Glasgow, Glasgow G61 1QH, UK
4
Edinburgh Genome Foundry, University of Edinburgh, Edinburgh EH9 3BF, UK
5
School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Nottingham LE12 5RD, UK
*
Author to whom correspondence should be addressed.
Academic Editors: Noemí Sevilla, Verónica Martín and José M. Rojas
Viruses 2021, 13(5), 919; https://doi.org/10.3390/v13050919
Received: 25 March 2021 / Revised: 4 May 2021 / Accepted: 7 May 2021 / Published: 15 May 2021
(This article belongs to the Special Issue Bluetongue Virus: Pathogenesis and Vaccines)
Arboviruses such as bluetongue virus (BTV) replicate in arthropod vectors involved in their transmission between susceptible vertebrate-hosts. The “classical” BTV strains infect and replicate effectively in cells of their insect-vectors (Culicoides biting-midges), as well as in those of their mammalian-hosts (ruminants). However, in the last decade, some “atypical” BTV strains, belonging to additional serotypes (e.g., BTV-26), have been found to replicate efficiently only in mammalian cells, while their replication is severely restricted in Culicoides cells. Importantly, there is evidence that these atypical BTV are transmitted by direct-contact between their mammalian hosts. Here, the viral determinants and mechanisms restricting viral replication in Culicoides were investigated using a classical BTV-1, an “atypical” BTV-26 and a BTV-1/BTV-26 reassortant virus, derived by reverse genetics. Viruses containing the capsid of BTV-26 showed a reduced ability to attach to Culicoides cells, blocking early steps of the replication cycle, while attachment and replication in mammalian cells was not restricted. The replication of BTV-26 was also severely reduced in other arthropod cells, derived from mosquitoes or ticks. The data presented identifies mechanisms and potential barriers to infection and transmission by the newly emerged “atypical” BTV strains in Culicoides. View Full-Text
Keywords: arbovirus; bluetongue; BTV-26; atypical serotype; reverse genetics; cell binding; VP2 arbovirus; bluetongue; BTV-26; atypical serotype; reverse genetics; cell binding; VP2
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MDPI and ACS Style

Guimerà Busquets, M.; Pullinger, G.D.; Darpel, K.E.; Cooke, L.; Armstrong, S.; Simpson, J.; Palmarini, M.; Fragkoudis, R.; Mertens, P.P.C. An Early Block in the Replication of the Atypical Bluetongue Virus Serotype 26 in Culicoides Cells Is Determined by Its Capsid Proteins. Viruses 2021, 13, 919. https://doi.org/10.3390/v13050919

AMA Style

Guimerà Busquets M, Pullinger GD, Darpel KE, Cooke L, Armstrong S, Simpson J, Palmarini M, Fragkoudis R, Mertens PPC. An Early Block in the Replication of the Atypical Bluetongue Virus Serotype 26 in Culicoides Cells Is Determined by Its Capsid Proteins. Viruses. 2021; 13(5):919. https://doi.org/10.3390/v13050919

Chicago/Turabian Style

Guimerà Busquets, Marc, Gillian D. Pullinger, Karin E. Darpel, Lyndsay Cooke, Stuart Armstrong, Jennifer Simpson, Massimo Palmarini, Rennos Fragkoudis, and Peter P. C. Mertens. 2021. "An Early Block in the Replication of the Atypical Bluetongue Virus Serotype 26 in Culicoides Cells Is Determined by Its Capsid Proteins" Viruses 13, no. 5: 919. https://doi.org/10.3390/v13050919

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