Next Article in Journal
Genomic Evidence for Sequestration of Influenza A Virus Lineages in Sea Duck Host Species
Next Article in Special Issue
Comparative Analysis of Within-Host Mutation Patterns and Diversity of Hepatitis C Virus Subtypes 1a, 1b, and 3a
Previous Article in Journal
Innate Immune Sensing of Viruses and Its Consequences for the Central Nervous System
Previous Article in Special Issue
Therapy Implications of Hepatitis C Virus Genetic Diversity
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Viruses
Volume 13
Issue 2
10.3390/v13020171
viruses-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

HIV-1 Gag-Pol Sequences from Ugandan Early Infections Reveal Sequence Variants Associated with Elevated Replication Capacity

by
Anne Kapaata
1,
Sheila N. Balinda
1,
Rui Xu
2,
Maria G. Salazar
1,
Kimberly Herard
2,
Kelsie Brooks
2,
Kato Laban
1,
Jonathan Hare
3,4,
Dario Dilernia
2,
Anatoli Kamali
5,
Eugene Ruzagira
1,
Freddie Mukasa
1,
Jill Gilmour
3,4,
Jesus F. Salazar-Gonzalez
1,
Ling Yue
2,
Matthew Cotten
1,6,*,
Eric Hunter
2 and
Pontiano Kaleebu
1
1
Medical Research Council, UVRI & LSTHM Uganda Research Unit, Plot 51–59, Entebbe, Uganda
2
Emory University, Atlanta, GA 30322, USA
3
Imperial College London, London SW7 2AZ, UK
4
International AIDS Vaccine Initiative (IAVI), New York, NY 10004, USA
5
IAVI, Nairobi 00202, Kenya
6
Centre for Virus Research, MRC-University of Glasgow, Glasgow G61 1QH, UK
*
Author to whom correspondence should be addressed.
Viruses 2021, 13(2), 171; https://doi.org/10.3390/v13020171
Submission received: 22 November 2020 / Revised: 4 January 2021 / Accepted: 6 January 2021 / Published: 23 January 2021
(This article belongs to the Special Issue Diversity and Evolution of HIV and HCV)
Browse Figures
Versions Notes

Abstract

The ability to efficiently establish a new infection is a critical property for human immunodeficiency virus type 1 (HIV-1). Although the envelope protein of the virus plays an essential role in receptor binding and internalization of the infecting virus, the structural proteins, the polymerase and the assembly of new virions may also play a role in establishing and spreading viral infection in a new host. We examined Ugandan viruses from newly infected patients and focused on the contribution of the Gag-Pol genes to replication capacity. A panel of Gag-Pol sequences generated using single genome amplification from incident HIV-1 infections were cloned into a common HIV-1 NL4.3 pol/env backbone and the influence of Gag-Pol changes on replication capacity was monitored. Using a novel protein domain approach, we then documented diversity in the functional protein domains across the Gag-Pol region and identified differences in the Gag-p6 domain that were frequently associated with higher in vitro replication.
Keywords: HIV-1; Uganda; recombinant; Gag-Pol; protein domains HIV-1; Uganda; recombinant; Gag-Pol; protein domains

Share and Cite

MDPI and ACS Style

Kapaata, A.; Balinda, S.N.; Xu, R.; Salazar, M.G.; Herard, K.; Brooks, K.; Laban, K.; Hare, J.; Dilernia, D.; Kamali, A.; et al. HIV-1 Gag-Pol Sequences from Ugandan Early Infections Reveal Sequence Variants Associated with Elevated Replication Capacity. Viruses 2021, 13, 171. https://doi.org/10.3390/v13020171

AMA Style

Kapaata A, Balinda SN, Xu R, Salazar MG, Herard K, Brooks K, Laban K, Hare J, Dilernia D, Kamali A, et al. HIV-1 Gag-Pol Sequences from Ugandan Early Infections Reveal Sequence Variants Associated with Elevated Replication Capacity. Viruses. 2021; 13(2):171. https://doi.org/10.3390/v13020171

Chicago/Turabian Style

Kapaata, Anne, Sheila N. Balinda, Rui Xu, Maria G. Salazar, Kimberly Herard, Kelsie Brooks, Kato Laban, Jonathan Hare, Dario Dilernia, Anatoli Kamali, and et al. 2021. "HIV-1 Gag-Pol Sequences from Ugandan Early Infections Reveal Sequence Variants Associated with Elevated Replication Capacity" Viruses 13, no. 2: 171. https://doi.org/10.3390/v13020171

APA Style

Kapaata, A., Balinda, S. N., Xu, R., Salazar, M. G., Herard, K., Brooks, K., Laban, K., Hare, J., Dilernia, D., Kamali, A., Ruzagira, E., Mukasa, F., Gilmour, J., Salazar-Gonzalez, J. F., Yue, L., Cotten, M., Hunter, E., & Kaleebu, P. (2021). HIV-1 Gag-Pol Sequences from Ugandan Early Infections Reveal Sequence Variants Associated with Elevated Replication Capacity. Viruses, 13(2), 171. https://doi.org/10.3390/v13020171

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop