Demonstration of Co-Infection and Trans-Encapsidation of Viral RNA In Vitro Using Epitope-Tagged Foot-and-Mouth Disease Viruses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Western Blotting
2.3. Immunofluorescence
2.4. Flow Cytometry
2.5. Immunoprecipitation
2.6. RT-PCR
3. Results
3.1. Co-Infection of Cells with Epitope-Tagged Viruses
3.2. Co-Infection with Epitope-Tagged FMDVs of Different Serotypes
3.3. Generation of Chimeric Capsids
3.4. Trans-Encapsidation of FMDV Genomes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Childs, K.; Juleff, N.; Moffat, K.; Seago, J. Demonstration of Co-Infection and Trans-Encapsidation of Viral RNA In Vitro Using Epitope-Tagged Foot-and-Mouth Disease Viruses. Viruses 2021, 13, 2433. https://doi.org/10.3390/v13122433
Childs K, Juleff N, Moffat K, Seago J. Demonstration of Co-Infection and Trans-Encapsidation of Viral RNA In Vitro Using Epitope-Tagged Foot-and-Mouth Disease Viruses. Viruses. 2021; 13(12):2433. https://doi.org/10.3390/v13122433
Chicago/Turabian StyleChilds, Kay, Nicholas Juleff, Katy Moffat, and Julian Seago. 2021. "Demonstration of Co-Infection and Trans-Encapsidation of Viral RNA In Vitro Using Epitope-Tagged Foot-and-Mouth Disease Viruses" Viruses 13, no. 12: 2433. https://doi.org/10.3390/v13122433