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Brief Report

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021

1
Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA
2
Connecticut Veterinary Medical Diagnostic Laboratory, Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Alice Fusaro and Isabella Monne
Viruses 2021, 13(11), 2141; https://doi.org/10.3390/v13112141
Received: 17 March 2021 / Revised: 19 October 2021 / Accepted: 21 October 2021 / Published: 23 October 2021
(This article belongs to the Special Issue Drivers of Evolution of Animal RNA Viruses)
We report the first detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a 3-month-old dog in Connecticut that died suddenly and was submitted to the state veterinary diagnostic laboratory for postmortem examination. Viral RNA was detected in multiple organs of the dog by reverse transcription real time-PCR (RT-qPCR). Negative and positive sense strands of viral RNA were visualized by in situ hybridization using RNAscope technology. Complete genome sequencing and phylogenetic analysis of the hCoV-19/USA/CT-CVMDL-Dog-1/2021 (CT_Dog/2021) virus were conducted to identify the origin and lineage of the virus. The CT_Dog/2021 virus belonged to the GH/B1.2. genetic lineage and was genetically similar to SARS-CoV-2 identified in humans in the U.S. during the winter of 2020–2021. However, it was not related to other SARS-CoV-2 variants identified from companion animals in the U.S. It contained both the D614G in spike and P323L in nsp12 substitutions, which have become the dominant mutations in the United States. The continued sporadic detections of SARS-CoV-2 in companion animals warrant public health concerns about the zoonotic potential of SARS-CoV-2 and enhance our collective understanding of the epidemiology of the virus. View Full-Text
Keywords: SARS-CoV-2; COVID-19; dog; sudden death; Connecticut; epidemiology; RNAscope SARS-CoV-2; COVID-19; dog; sudden death; Connecticut; epidemiology; RNAscope
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MDPI and ACS Style

Lee, D.-H.; Helal, Z.H.; Kim, J.; Hunt, A.; Barbieri, A.; Tocco, N.; Frasca, S., Jr.; Kerr, K.; Hyeon, J.-Y.; Chung, D.H.; Risatti, G. Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021. Viruses 2021, 13, 2141. https://doi.org/10.3390/v13112141

AMA Style

Lee D-H, Helal ZH, Kim J, Hunt A, Barbieri A, Tocco N, Frasca S Jr., Kerr K, Hyeon J-Y, Chung DH, Risatti G. Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021. Viruses. 2021; 13(11):2141. https://doi.org/10.3390/v13112141

Chicago/Turabian Style

Lee, Dong-Hun, Zeinab H. Helal, Junwon Kim, Amelia Hunt, Alyza Barbieri, Natalie Tocco, Salvatore Frasca Jr., Kirklyn Kerr, Ji-Yeon Hyeon, David H. Chung, and Guillermo Risatti. 2021. "Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021" Viruses 13, no. 11: 2141. https://doi.org/10.3390/v13112141

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