Trim24 and Trim33 Play a Role in Epigenetic Silencing of Retroviruses in Embryonic Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Differentiation
2.2. Stable shRNA and Overexpression Cell Line Production and Transduction
2.3. Co-Immunoprecipitation
2.4. RNA Extraction, Reverse Transcription and Real-Time PCR
2.5. Chromatin Immunoprecipitation
2.6. Flow Cytometry
2.7. Bisulfite Analysis
3. Results
3.1. Tif1 Family Members Are Co-Expressed and Bind to Each Other in ESCs
3.2. The Tif1 Complex Contribute to the Silencing of Some ERVs Subfamilies
3.3. Decrease in Expression and Epigenetic Silencing of Pro-Viral Sequences Following Trim24 or Trim33 Depletion
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Margalit, L.; Strauss, C.; Tal, A.; Schlesinger, S. Trim24 and Trim33 Play a Role in Epigenetic Silencing of Retroviruses in Embryonic Stem Cells. Viruses 2020, 12, 1015. https://doi.org/10.3390/v12091015
Margalit L, Strauss C, Tal A, Schlesinger S. Trim24 and Trim33 Play a Role in Epigenetic Silencing of Retroviruses in Embryonic Stem Cells. Viruses. 2020; 12(9):1015. https://doi.org/10.3390/v12091015
Chicago/Turabian StyleMargalit, Liad, Carmit Strauss, Ayellet Tal, and Sharon Schlesinger. 2020. "Trim24 and Trim33 Play a Role in Epigenetic Silencing of Retroviruses in Embryonic Stem Cells" Viruses 12, no. 9: 1015. https://doi.org/10.3390/v12091015