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Oropouche Virus Infects, Persists and Induces IFN Response in Human Peripheral Blood Mononuclear Cells as Identified by RNA PrimeFlow™ and qRT-PCR Assays

1
Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas 13083-862, Brazil
2
Department of Cell Biology and Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14049-900, Brazil
3
Leônidas and Maria Deane Institute (ILMD), Fiocruz Amazônia, Manaus 69057-070, Brazil
4
Hematology and Hemotherapy Center, Medical School of the University of Campinas, Campinas 13083-887, Brazil
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(7), 785; https://doi.org/10.3390/v12070785
Received: 20 May 2020 / Revised: 4 June 2020 / Accepted: 5 June 2020 / Published: 21 July 2020
Oropouche orthobunyavirus (OROV) is an emerging arbovirus with a high potential of dissemination in America. Little is known about the role of peripheral blood mononuclear cells (PBMC) response during OROV infection in humans. Thus, to evaluate human leukocytes susceptibility, permissiveness and immune response during OROV infection, we applied RNA hybridization, qRT-PCR and cell-based assays to quantify viral antigens, genome, antigenome and gene expression in different cells. First, we observed OROV replication in human leukocytes lineages as THP-1 monocytes, Jeko-1 B cells and Jurkat T cells. Interestingly, cell viability and viral particle detection are maintained in these cells, even after successive passages. PBMCs from healthy donors were susceptible but the infection was not productive, since neither antigenome nor infectious particle was found in the supernatant of infected PBMCs. In fact, only viral antigens and small quantities of OROV genome were detected at 24 hpi in lymphocytes, monocytes and CD11c+ cells. Finally, activation of the Interferon (IFN) response was essential to restrict OROV replication in human PBMCs. Increased expression of type I/III IFNs, ISGs and inflammatory cytokines was detected in the first 24 hpi and viral replication was re-established after blocking IFNAR or treating cells with glucocorticoid. Thus, in short, our results show OROV is able to infect and remain in low titers in human T cells, monocytes, DCs and B cells as a consequence of an effective IFN response after infection, indicating the possibility of leukocytes serving as a trojan horse in specific microenvironments during immunosuppression. View Full-Text
Keywords: Oropouche virus; PBMC; lymphocyte; interferons; RNA PrimeFlow™; dendritic cells; monocytes; B cells Oropouche virus; PBMC; lymphocyte; interferons; RNA PrimeFlow™; dendritic cells; monocytes; B cells
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MDPI and ACS Style

Ribeiro Amorim, M.; Cornejo Pontelli, M.; Fabiano de Souza, G.; Primon Muraro, S.; Toledo-Teixeira, D.A.; Forato, J.; Bispo-dos-Santos, K.; Barbosa, N.S.; Cavalheiro Martini, M.; Lorencini Parise, P.; Vieira, A.; Paier Milanez, G.; Lamberti Pinto daSilva, L.; Jaychand Lalwani, P.; Santos Farias, A.; Ramirez Vinolo, M.A.; Sesti-Costa, R.; Arruda, E.; Proenca-Modena, J.L. Oropouche Virus Infects, Persists and Induces IFN Response in Human Peripheral Blood Mononuclear Cells as Identified by RNA PrimeFlow™ and qRT-PCR Assays. Viruses 2020, 12, 785. https://doi.org/10.3390/v12070785

AMA Style

Ribeiro Amorim M, Cornejo Pontelli M, Fabiano de Souza G, Primon Muraro S, Toledo-Teixeira DA, Forato J, Bispo-dos-Santos K, Barbosa NS, Cavalheiro Martini M, Lorencini Parise P, Vieira A, Paier Milanez G, Lamberti Pinto daSilva L, Jaychand Lalwani P, Santos Farias A, Ramirez Vinolo MA, Sesti-Costa R, Arruda E, Proenca-Modena JL. Oropouche Virus Infects, Persists and Induces IFN Response in Human Peripheral Blood Mononuclear Cells as Identified by RNA PrimeFlow™ and qRT-PCR Assays. Viruses. 2020; 12(7):785. https://doi.org/10.3390/v12070785

Chicago/Turabian Style

Ribeiro Amorim, Mariene, Marjorie Cornejo Pontelli, Gabriela Fabiano de Souza, Stéfanie Primon Muraro, Daniel A. Toledo-Teixeira, Julia Forato, Karina Bispo-dos-Santos, Natália S. Barbosa, Matheus Cavalheiro Martini, Pierina Lorencini Parise, Aline Vieira, Guilherme Paier Milanez, Luis Lamberti Pinto daSilva, Pritesh Jaychand Lalwani, Alessandro Santos Farias, Marco A. Ramirez Vinolo, Renata Sesti-Costa, Eurico Arruda, and Jose L. Proenca-Modena. 2020. "Oropouche Virus Infects, Persists and Induces IFN Response in Human Peripheral Blood Mononuclear Cells as Identified by RNA PrimeFlow™ and qRT-PCR Assays" Viruses 12, no. 7: 785. https://doi.org/10.3390/v12070785

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