Conserved Oligomeric Golgi (COG) Complex Proteins Facilitate Orthopoxvirus Entry, Fusion and Spread
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Immunostaining
2.3. Multi-Step Infections
2.4. Plaque Assays
2.5. Confocal Imaging
2.6. Luciferase-Based Entry Assay
2.7. Membrane Fusion Assay
2.8. Statistical Analysis
3. Results
3.1. Reduced Size of MPXV Foci in COG KO Cells
3.2. Reduced MPXV Yield in COG KO Cells
3.3. VACV Entry Reduced in COG KO Cells
3.4. COG Complex Plays a Role in VACV Egress
3.5. Ectopic Expression of COG4 and COG7 Restores Virus Spread
3.6. Entry of MV and EV VACV Particles is Reduced in Cells Lacking COG4 and COG7
3.7. COG4 and COG7 are Important for Viral Fusion
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Realegeno, S.; Priyamvada, L.; Kumar, A.; Blackburn, J.B.; Hartloge, C.; Puschnik, A.S.; Sambhara, S.; Olson, V.A.; Carette, J.E.; Lupashin, V.; et al. Conserved Oligomeric Golgi (COG) Complex Proteins Facilitate Orthopoxvirus Entry, Fusion and Spread. Viruses 2020, 12, 707. https://doi.org/10.3390/v12070707
Realegeno S, Priyamvada L, Kumar A, Blackburn JB, Hartloge C, Puschnik AS, Sambhara S, Olson VA, Carette JE, Lupashin V, et al. Conserved Oligomeric Golgi (COG) Complex Proteins Facilitate Orthopoxvirus Entry, Fusion and Spread. Viruses. 2020; 12(7):707. https://doi.org/10.3390/v12070707
Chicago/Turabian StyleRealegeno, Susan, Lalita Priyamvada, Amrita Kumar, Jessica B. Blackburn, Claire Hartloge, Andreas S. Puschnik, Suryaprakash Sambhara, Victoria A. Olson, Jan E. Carette, Vladimir Lupashin, and et al. 2020. "Conserved Oligomeric Golgi (COG) Complex Proteins Facilitate Orthopoxvirus Entry, Fusion and Spread" Viruses 12, no. 7: 707. https://doi.org/10.3390/v12070707