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Open AccessArticle

Non-Mouse-Adapted H1N1pdm09 Virus as a Model for Influenza Research

1
Department of Virology, Federal State Budgetary Scientific Institution “Institute of Experimental Medicine”, 197376 St Petersburg, Russia
2
Department of Toxicology and Microbiology, Institute of Preclinical Research Ltd., 188663 St Petersburg, Russia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(6), 590; https://doi.org/10.3390/v12060590
Received: 30 April 2020 / Revised: 21 May 2020 / Accepted: 27 May 2020 / Published: 29 May 2020
(This article belongs to the Section Animal Viruses)
The number of lung-adapted influenza viruses is limited. Most of them are not antigenically related to current circulating viruses. Viruses similar to recent strains are required for screening modern antiviral compounds and studying new vaccine candidates against novel influenza viruses. The process by which an influenza virus adapts to a new host is rather difficult. The aim of this study was to select a non-adapted current virus whose major biological properties correspond to those of classical lab-adapted viruses. Mice were inoculated intranasally with non-lung-adapted influenza viruses of subtype H1N1pdm09. They were monitored closely for body weight loss, mortality outcomes and gross pathology for 14 days following inoculation, as well as viral replication in lung tissue. Lung-adapted PR8 virus was used as a control. The tested viruses multiplied equally well in the lower respiratory tract of mice without prior adaptation but dramatically differed in lethality; the differences in their toxicity and pathogenicity in mice were established. A/South Africa/3626/2013 (H1N1)pdm09 virus was found to be an appropriate candidate to replace PR8 as a model virus for influenza research. No prior adaptation to the animal model is needed to reach the pathogenicity level of the classical mouse-adapted PR8 virus. View Full-Text
Keywords: influenza virus; influenza infection; animal model; viral toxicity; viral pathogenesis influenza virus; influenza infection; animal model; viral toxicity; viral pathogenesis
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MDPI and ACS Style

Kiseleva, I.; Rekstin, A.; Al Farroukh, M.; Bazhenova, E.; Katelnikova, A.; Puchkova, L.; Rudenko, L. Non-Mouse-Adapted H1N1pdm09 Virus as a Model for Influenza Research. Viruses 2020, 12, 590. https://doi.org/10.3390/v12060590

AMA Style

Kiseleva I, Rekstin A, Al Farroukh M, Bazhenova E, Katelnikova A, Puchkova L, Rudenko L. Non-Mouse-Adapted H1N1pdm09 Virus as a Model for Influenza Research. Viruses. 2020; 12(6):590. https://doi.org/10.3390/v12060590

Chicago/Turabian Style

Kiseleva, Irina; Rekstin, Andrey; Al Farroukh, Mohammad; Bazhenova, Ekaterina; Katelnikova, Anastasia; Puchkova, Ludmila; Rudenko, Larisa. 2020. "Non-Mouse-Adapted H1N1pdm09 Virus as a Model for Influenza Research" Viruses 12, no. 6: 590. https://doi.org/10.3390/v12060590

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