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Open AccessArticle

Hepatitis B Virus-X Downregulates Expression of Selenium Binding Protein 1

1
Dasan Undergraduate College, Ajou University, Suwon 16499, Korea
2
Graduate School of New Drug Discovery & Development, Chungnam National University, Daejeon 34134, Korea
3
Department of Smart Food & Drugs, Inje University, Gimhae 50834, Korea
*
Author to whom correspondence should be addressed.
These authors contribute equally in this work.
Viruses 2020, 12(5), 565; https://doi.org/10.3390/v12050565
Received: 26 April 2020 / Revised: 15 May 2020 / Accepted: 18 May 2020 / Published: 20 May 2020
(This article belongs to the Special Issue Hepatitis B Virus: From Diagnostics to Treatments)
Selenium binding protein 1 (SELENBP1) has been known to be reduced in various types cancer, and epigenetic change is shown to be likely to account for the reduction of SELNEBP1 expression. With cDNA microarray comparative analysis, we found that SELENBP1 is markedly decreased in hepatitis B virus-X (HBx)-expressing cells. To clarify the effect of HBx on SELENBP1 expression, we compared the expression levels of SELENBP1 mRNA and protein by semi-quantitative RT-PCR, Northern blot, and Western blot. As expected, SELENBP1 expression was shown to be reduced in cells expressing HBx, and reporter gene analysis showed that the SELENBP1 promoter is repressed by HBx. In addition, the stepwise deletion of 5' flanking promoter sequences resulted in a gradual decrease in basal promoter activity and inhibition of SELENBP1 expression by HBx. Moreover, immunohistochemistry on tissue microarrays containing 60 pairs of human liver tissue showed decreased intensity of SELENBP1 in tumor tissues as compared with their matched non-tumor liver tissues. Taken together, our findings suggest that inhibition of SELENBP1 expression by HBx might act as one of the causes in the development of hepatocellular carcinoma caused by HBV infection.
Keywords: hepatitis B virus; X protein; selenium binding protein 1; hepatocellular carcinoma hepatitis B virus; X protein; selenium binding protein 1; hepatocellular carcinoma
MDPI and ACS Style

Lee, Y.-M.; Kim, S.; Park, R.-Y.; Kim, Y.-S. Hepatitis B Virus-X Downregulates Expression of Selenium Binding Protein 1. Viruses 2020, 12, 565.

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