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Fluorescence Microscopy of the HIV-1 Envelope

by Pablo Carravilla 1,2,3,4,*, José L. Nieva 3,4 and Christian Eggeling 1,2,5,6
1
Institute of Applied Optics and Biophysics, Friedrich-Schiller-University Jena, Max-Wien Platz 1, 07743 Jena, Germany
2
Leibniz Institute of Photonic Technology, Albert Einstein Strasse 9, 07743 Jena, Germany
3
Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU), P.O. Box 644, 48080 Bilbao, Spain
4
Instituto Biofisika (UPV/EHU, CSIC), University of the Basque Country, E-48940 Leioa, Spain
5
MRC Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK
6
Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University Jena, Philosophenweg 7, 07743 Jena, Germany
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(3), 348; https://doi.org/10.3390/v12030348 (registering DOI)
Received: 22 February 2020 / Revised: 19 March 2020 / Accepted: 20 March 2020 / Published: 21 March 2020
(This article belongs to the Special Issue Mechanisms of Viral Fusion and Applications in Antivirals)
Human immunodeficiency virus (HIV) infection constitutes a major health and social issue worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane. This process is mediated by the viral Env glycoprotein and depends on the envelope lipid composition. Fluorescent microscopy has been employed to investigate the envelope properties, and the processes of viral assembly and fusion, but the application of this technique to the study of HIV is still limited by a number of factors, such as the small size of HIV virions or the difficulty to label the envelope components. Here, we review fluorescence imaging studies of the envelope lipids and proteins, focusing on labelling strategies and model systems. View Full-Text
Keywords: microscopy; HIV; envelope; lipid; membrane; Env; fluorescence; super-resolution microscopy; HIV; envelope; lipid; membrane; Env; fluorescence; super-resolution
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Carravilla, P.; Nieva, J.L.; Eggeling, C. Fluorescence Microscopy of the HIV-1 Envelope. Viruses 2020, 12, 348.

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