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Open AccessArticle

Common and Strain-Specific Post-Translational Modifications of the Potyvirus Plum pox virus Coat Protein in Different Hosts

1
Department of Plant Molecular Genetics, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain
2
Proteomics Unit, Centro Nacional de Biotecnología (CNB-CSIC), ProteoRed ISCIII, 28049 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Current address: NIM Genetics, st/Anabel Segura 16, 28108 Alcobendas, Spain.
Viruses 2020, 12(3), 308; https://doi.org/10.3390/v12030308
Received: 24 January 2020 / Revised: 5 March 2020 / Accepted: 9 March 2020 / Published: 12 March 2020
(This article belongs to the Special Issue The Complexity of the Potyviral Interaction Network)
Phosphorylation and O-GlcNAcylation are widespread post-translational modifications (PTMs), often sharing protein targets. Numerous studies have reported the phosphorylation of plant viral proteins. In plants, research on O-GlcNAcylation lags behind that of other eukaryotes, and information about O-GlcNAcylated plant viral proteins is extremely scarce. The potyvirus Plum pox virus (PPV) causes sharka disease in Prunus trees and also infects a wide range of experimental hosts. Capsid protein (CP) from virions of PPV-R isolate purified from herbaceous plants can be extensively modified by O-GlcNAcylation and phosphorylation. In this study, a combination of proteomics and biochemical approaches was employed to broaden knowledge of PPV CP PTMs. CP proved to be modified regardless of whether or not it was assembled into mature particles. PTMs of CP occurred in the natural host Prunus persica, similarly to what happens in herbaceous plants. Additionally, we observed that O-GlcNAcylation and phosphorylation were general features of different PPV strains, suggesting that these modifications contribute to general strategies deployed during plant-virus interactions. Interestingly, phosphorylation at a casein kinase II motif conserved among potyviral CPs exhibited strain specificity in PPV; however, it did not display the critical role attributed to the same modification in the CP of another potyvirus, Potato virus A. View Full-Text
Keywords: capsid protein; O-GlcNAcylation; phosphorylation; sharka; potyvirus capsid protein; O-GlcNAcylation; phosphorylation; sharka; potyvirus
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MDPI and ACS Style

Hervás, M.; Ciordia, S.; Navajas, R.; García, J.A.; Martínez-Turiño, S. Common and Strain-Specific Post-Translational Modifications of the Potyvirus Plum pox virus Coat Protein in Different Hosts. Viruses 2020, 12, 308. https://doi.org/10.3390/v12030308

AMA Style

Hervás M, Ciordia S, Navajas R, García JA, Martínez-Turiño S. Common and Strain-Specific Post-Translational Modifications of the Potyvirus Plum pox virus Coat Protein in Different Hosts. Viruses. 2020; 12(3):308. https://doi.org/10.3390/v12030308

Chicago/Turabian Style

Hervás, Marta; Ciordia, Sergio; Navajas, Rosana; García, Juan A.; Martínez-Turiño, Sandra. 2020. "Common and Strain-Specific Post-Translational Modifications of the Potyvirus Plum pox virus Coat Protein in Different Hosts" Viruses 12, no. 3: 308. https://doi.org/10.3390/v12030308

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