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Special Issue “Emerging Viruses: Surveillance, Prevention, Evolution, and Control”
Open AccessArticle

Role of CARD Region of MDA5 Gene in Canine Influenza Virus Infection

by 1,2,3,4, 1,3,4, 1,3,4 and 1,3,4,*
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou 510642, China
Guangdong Technological Engineering Research Center for Pet, Guangzhou 510642, China
Author to whom correspondence should be addressed.
Viruses 2020, 12(3), 307;
Received: 17 January 2020 / Revised: 25 February 2020 / Accepted: 9 March 2020 / Published: 12 March 2020
(This article belongs to the Special Issue Influenza A Virus: Host-Virus Relationship)
MDA5 belongs to the RIG-I-like receptor family, which is involved in innate immunity. During viral infection, MDA5 generates an antiviral response by recognizing the ligand to activate interferon. However, the role and mechanism of MDA5 in canine influenza virus (CIV) infection are unclear. To understand the mechanism of canine MDA5-mediated innate immunity during CIV infection, we detected the distribution of MDA5 in beagles, and the structural prediction showed that MDA5 was mainly composed of a CARD domain, RD domain, and DExD/H helix structure. Moreover, we found that MDA5 inhibits CIV replication. Furthermore, in the dual luciferase assay, we revealed that the CARD region of MDA5 strongly activated the IFN-β promoter and mainly transmitted signals through the CARD region. Overexpression of the CARD region of MDA5 revealed that the MDA5-mediated signaling pathway could transmit signals by activating the IRF3/NF-κB and IRF3 promoters, promoting the expression of antiviral proteins and cytokine release, thereby inhibiting CIV replication. Upon silencing of MDA5, cytokine production decreased, while the replication ability of CIV was increased. Thus, this study revealed a novel mechanism by which MDA5 mediated CIV infection and provided new avenues for the development of antiviral strategies. View Full-Text
Keywords: MDA5; canine influenza virus; antiviral activity; innate immunity MDA5; canine influenza virus; antiviral activity; innate immunity
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MDPI and ACS Style

Fu, C.; Ye, S.; Liu, Y.; Li, S. Role of CARD Region of MDA5 Gene in Canine Influenza Virus Infection. Viruses 2020, 12, 307.

AMA Style

Fu C, Ye S, Liu Y, Li S. Role of CARD Region of MDA5 Gene in Canine Influenza Virus Infection. Viruses. 2020; 12(3):307.

Chicago/Turabian Style

Fu, Cheng; Ye, Shaotang; Liu, Yongbo; Li, Shoujun. 2020. "Role of CARD Region of MDA5 Gene in Canine Influenza Virus Infection" Viruses 12, no. 3: 307.

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