Structures of Three Actinobacteriophage Capsids: Roles of Symmetry and Accessory Proteins
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacteriophages, Hosts, and Sources
2.2. Production of Bacteriophages for Cryo-Electron Microscopy and Mass Spectrometry
2.3. Preparation of Bacteriophages for Transmission Electron Microscopy
2.4. Preparation of Cryo-Electron Microscopy Grids
2.5. Cryo-Electron Microscopy
2.6. Cryo-Electron Microscopy Data Analysis
2.6.1. Data Deposition
2.7. Bioinformatics
2.8. Mass Spectrometry
2.8.1. In-Gel Digestions, Tryptic Peptide Extraction and Desalting
2.8.2. Peptide and Protein Identification Using Mass Spectrometry
3. Results
3.1. Virion Morphologies
3.2. Capsid Morphologies and Accessory Proteins
3.3. SDS-PAGE Gel and Mass Spectrometry Analysis of Rosebush
3.4. Bioinformatic Analysis of Accessory Protein gp17
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Name | Number of Genes | Cluster | Number of Phages in Cluster | Genome Length (bp) | Genome GC Content (%) | Life Cycle | Host | Morphology |
---|---|---|---|---|---|---|---|---|
Myrna | 229 | C2 | 2 | 164602 | 65.4 | Lytic | M. smegmatis mc2155 | Myoviridae |
Patience | 109 | U | 2 | 70506 | 50.3 | Lytic | M. smegmatis mc2155 | Siphoviridae |
Rosebush | 90 | B2 | 27 | 67480 | 68.9 | Lytic | M. smegmatis mc2155 | Siphoviridae |
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Podgorski, J.; Calabrese, J.; Alexandrescu, L.; Jacobs-Sera, D.; Pope, W.; Hatfull, G.; White, S. Structures of Three Actinobacteriophage Capsids: Roles of Symmetry and Accessory Proteins. Viruses 2020, 12, 294. https://doi.org/10.3390/v12030294
Podgorski J, Calabrese J, Alexandrescu L, Jacobs-Sera D, Pope W, Hatfull G, White S. Structures of Three Actinobacteriophage Capsids: Roles of Symmetry and Accessory Proteins. Viruses. 2020; 12(3):294. https://doi.org/10.3390/v12030294
Chicago/Turabian StylePodgorski, Jennifer, Joshua Calabrese, Lauren Alexandrescu, Deborah Jacobs-Sera, Welkin Pope, Graham Hatfull, and Simon White. 2020. "Structures of Three Actinobacteriophage Capsids: Roles of Symmetry and Accessory Proteins" Viruses 12, no. 3: 294. https://doi.org/10.3390/v12030294