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Volume 12
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10.3390/v12030283
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Article

Construction of eGFP-Tagged Senecavirus A for Facilitating Virus Neutralization Test and Antiviral Assay

by
Fuxiao Liu
1,2,*,†,
Yilan Huang
1,†,
Qianqian Wang
1,† and
Hu Shan
1,3,*
1
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China
2
Qingdao Research Center for Veterinary Biological Engineering and Technology, Qingdao 266109, China
3
Shandong Collaborative Innovation Center for Development of New Veterinary Pharmaceuticals, Qingdao 266109, China
*
Authors to whom correspondence should be addressed.
†
The contribution is equal to this work.
Viruses 2020, 12(3), 283; https://doi.org/10.3390/v12030283
Submission received: 5 February 2020 / Revised: 1 March 2020 / Accepted: 2 March 2020 / Published: 5 March 2020
(This article belongs to the Section Animal Viruses)
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Abstract

Senecavirus A (SVA), also known as Seneca Valley virus, is an emerging virus that causes vesicular disease in pigs. This virus belongs to the genus Senecavirus in the family Picornaviridae. The SVA CH-LX-01-2016 was isolated from Guangdong Province of China in 2016. In this study, a recombinant SVA CH-LX-01-2016 was constructed using reverse genetics, and proven to be able to express efficiently an enhanced green fluorescent protein (eGFP) in vitro. This eGFP-tagged recombinant SVA (rSVA-eGFP) exhibited a high capacity for viral replication. Its fluorescence-tracked characteristics greatly facilitated both virus neutralization test (VNT) and antiviral assay. The rSVA-eGFP-based VNT was used to detect eight porcine serum samples, out of which four were determined to be neutralization titer-positive. Subsequently, two antiviral drugs, ribavirin and apigenin, were assayed for evaluating both effects against the rSVA-eGFP in vitro. The result showed that only the ribavirin exhibited an anti-SVA activity.
Keywords: Senecavirus A; reverse genetics; eGFP; virus neutralization test; ribavirin; apigenin Senecavirus A; reverse genetics; eGFP; virus neutralization test; ribavirin; apigenin

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MDPI and ACS Style

Liu, F.; Huang, Y.; Wang, Q.; Shan, H. Construction of eGFP-Tagged Senecavirus A for Facilitating Virus Neutralization Test and Antiviral Assay. Viruses 2020, 12, 283. https://doi.org/10.3390/v12030283

AMA Style

Liu F, Huang Y, Wang Q, Shan H. Construction of eGFP-Tagged Senecavirus A for Facilitating Virus Neutralization Test and Antiviral Assay. Viruses. 2020; 12(3):283. https://doi.org/10.3390/v12030283

Chicago/Turabian Style

Liu, Fuxiao, Yilan Huang, Qianqian Wang, and Hu Shan. 2020. "Construction of eGFP-Tagged Senecavirus A for Facilitating Virus Neutralization Test and Antiviral Assay" Viruses 12, no. 3: 283. https://doi.org/10.3390/v12030283

APA Style

Liu, F., Huang, Y., Wang, Q., & Shan, H. (2020). Construction of eGFP-Tagged Senecavirus A for Facilitating Virus Neutralization Test and Antiviral Assay. Viruses, 12(3), 283. https://doi.org/10.3390/v12030283

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