Next Article in Journal
RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles
Next Article in Special Issue
Viromics on Honey-Baited FTA Cards as a New Tool for the Detection of Circulating Viruses in Mosquitoes
Previous Article in Journal
Characterization of Molecular Cluster Detection and Evaluation of Cluster Investigation Criteria Using Machine Learning Methods and Statewide Surveillance Data in Washington State
Previous Article in Special Issue
Virus Metagenomics in Farm Animals: A Systematic Review
Article

Virus Discovery in Desert Tortoise Fecal Samples: Novel Circular Single-Stranded DNA Viruses

1
School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA
2
The Biodesign Center for Fundamental and Applied Microbiomics, Arizona State University, Tempe, AZ 85287, USA
3
Natural Resources Program, Naval Facilities Engineering Command-Navy Region Southwest, San Diego, CA 92101, USA, USA
4
Department of Anthropology, University of Utah, Salt Lake City, UT 84112, USA
5
Center for Evolution and Medicine, Arizona State University, Tempe, AZ 85287, USA
6
Structural Biology Research Unit, Department of Clinical Laboratory Sciences, University of Cape Town, Cape Town 7925, South Africa
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(2), 143; https://doi.org/10.3390/v12020143
Received: 26 October 2019 / Revised: 18 January 2020 / Accepted: 21 January 2020 / Published: 26 January 2020
(This article belongs to the Special Issue Viromics: Approaches, Advances, and Applications)
The Sonoran Desert tortoise Gopherus morafkai is adapted to the desert, and plays an important ecological role in this environment. There is limited information on the viral diversity associated with tortoises (family Testudinidae), and to date no DNA virus has been identified associated with these animals. This study aimed to assess the diversity of DNA viruses associated with the Sonoran Desert tortoise by sampling their fecal matter. A viral metagenomics approach was used to identify the DNA viruses in fecal samples from wild Sonoran Desert tortoises in Arizona, USA. In total, 156 novel single-stranded DNA viruses were identified from 40 fecal samples. Those belonged to two known viral families, the Genomoviridae (n = 27) and Microviridae (n = 119). In addition, 10 genomes were recovered that belong to the unclassified group of circular-replication associated protein encoding single-stranded (CRESS) DNA virus and five circular molecules encoding viral-like proteins. View Full-Text
Keywords: Arizona; Genomoviridae; Microviridae; CRESS DNA viruses; Gopherus morafkai Arizona; Genomoviridae; Microviridae; CRESS DNA viruses; Gopherus morafkai
Show Figures

Figure 1

MDPI and ACS Style

Orton, J.P.; Morales, M.; Fontenele, R.S.; Schmidlin, K.; Kraberger, S.; Leavitt, D.J.; Webster, T.H.; Wilson, M.A.; Kusumi, K.; Dolby, G.A.; Varsani, A. Virus Discovery in Desert Tortoise Fecal Samples: Novel Circular Single-Stranded DNA Viruses. Viruses 2020, 12, 143. https://doi.org/10.3390/v12020143

AMA Style

Orton JP, Morales M, Fontenele RS, Schmidlin K, Kraberger S, Leavitt DJ, Webster TH, Wilson MA, Kusumi K, Dolby GA, Varsani A. Virus Discovery in Desert Tortoise Fecal Samples: Novel Circular Single-Stranded DNA Viruses. Viruses. 2020; 12(2):143. https://doi.org/10.3390/v12020143

Chicago/Turabian Style

Orton, Joseph P., Matheo Morales, Rafaela S. Fontenele, Kara Schmidlin, Simona Kraberger, Daniel J. Leavitt, Timothy H. Webster, Melissa A. Wilson, Kenro Kusumi, Greer A. Dolby, and Arvind Varsani. 2020. "Virus Discovery in Desert Tortoise Fecal Samples: Novel Circular Single-Stranded DNA Viruses" Viruses 12, no. 2: 143. https://doi.org/10.3390/v12020143

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop