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Review

Toward Tightly Tuned Gene Expression Following Lentiviral Vector Transduction

CIRI-Centre International de Recherche en Infectiologie, University of Lyon, Université Claude Bernard Lyon 1, Inserm, U1111, CNRS, UMR5308, ENS Lyon, 46 allée d’Italie, F-69007 Lyon, France
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Academic Editor: Yasuhiro Takeuchi
Viruses 2020, 12(12), 1427; https://doi.org/10.3390/v12121427
Received: 19 November 2020 / Revised: 5 December 2020 / Accepted: 8 December 2020 / Published: 11 December 2020
(This article belongs to the Special Issue Lentiviral Vectors)
Lentiviral vectors are versatile tools for gene delivery purposes. While in the earlier versions of retroviral vectors, transgene expression was controlled by the long terminal repeats (LTRs), the latter generations of vectors, including those derived from lentiviruses, incorporate internal constitutive or regulated promoters in order to regulate transgene expression. This allows to temporally and/or quantitatively control transgene expression, which is required for many applications such as for clinical applications, when transgene expression is required in specific tissues and at a specific timing. Here we review the main systems that have been developed for transgene regulated expression following lentiviral gene transfer. First, the induction of gene expression can be triggered either by external or by internal cues. Indeed, these regulated vector systems may harbor promoters inducible by exogenous stimuli, such as small molecules (e.g., antibiotics) or temperature variations, offering the possibility to tune rapidly transgene expression in case of adverse events. Second, expression can be indirectly adjusted by playing on inserted sequence copies, for instance by gene excision. Finally, synthetic networks can be developed to sense specific endogenous signals and trigger defined responses after information processing. Regulatable lentiviral vectors (LV)-mediated transgene expression systems have been widely used in basic research to uncover gene functions or to temporally reprogram cells. Clinical applications are also under development to induce therapeutic molecule secretion or to implement safety switches. Such regulatable approaches are currently focusing much attention and will benefit from the development of other technologies in order to launch autonomously controlled systems. View Full-Text
Keywords: lentiviral vectors; induction; transgene; signal; sensor; integration; promoter; synthetic biology lentiviral vectors; induction; transgene; signal; sensor; integration; promoter; synthetic biology
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MDPI and ACS Style

Page, A.; Fusil, F.; Cosset, F.-L. Toward Tightly Tuned Gene Expression Following Lentiviral Vector Transduction. Viruses 2020, 12, 1427. https://doi.org/10.3390/v12121427

AMA Style

Page A, Fusil F, Cosset F-L. Toward Tightly Tuned Gene Expression Following Lentiviral Vector Transduction. Viruses. 2020; 12(12):1427. https://doi.org/10.3390/v12121427

Chicago/Turabian Style

Page, Audrey, Floriane Fusil, and François-Loïc Cosset. 2020. "Toward Tightly Tuned Gene Expression Following Lentiviral Vector Transduction" Viruses 12, no. 12: 1427. https://doi.org/10.3390/v12121427

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