Next Article in Journal
Marek’s Disease Virus Cluster 3 miRNAs Restrict Virus’ Early Cytolytic Replication and Pathogenesis
Previous Article in Journal
Recombination and Positive Selection Differentially Shaped the Diversity of Betacoronavirus Subgenera
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:

Detection of SARS-CoV-2 from Saliva as Compared to Nasopharyngeal Swabs in Outpatients

Mount Sinai Hospital, Toronto, ON M5G 1X5, Canada
Department of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada
Department of Medicine, Michael Garron Hospital, Toronto, ON M4C 3E7, Canada
Dynacare Laboratory, Brampton, ON L6T 5M3, Canada
Department of Laboratory Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada
Shared Hospital Laboratory, Toronto, ON M4N 3M5, Canada
North York General Hospital, Toronto, ON M2K 1E1, Canada
Sunnybrook Health Sciences Centre, Toronto, ON M4N 3M5, Canada
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(11), 1314;
Received: 23 October 2020 / Revised: 13 November 2020 / Accepted: 14 November 2020 / Published: 17 November 2020
(This article belongs to the Section SARS-CoV-2 and COVID-19)


Widely available and easily accessible testing for COVID-19 is a cornerstone of pandemic containment strategies. Nasopharyngeal swabs (NPS) are the currently accepted standard for sample collection but are limited by their need for collection devices and sampling by trained healthcare professionals. The aim of this study was to compare the performance of saliva to NPS in an outpatient setting. This was a prospective study conducted at three centers, which compared the performance of saliva and NPS samples collected at the time of assessment center visit. Samples were tested by real-time reverse transcription polymerase chain reaction and sensitivity and overall agreement determined between saliva and NPS. Clinical data was abstracted by chart review for select study participants. Of the 432 paired samples, 46 were positive for SARS-CoV-2, with seven discordant observed between the two sample types (four individuals testing positive only by NPS and three by saliva only). The observed agreement was 98.4% (kappa coefficient 0.91) and a composite reference standard demonstrated sensitivity of 0.91 and 0.93 for saliva and NPS samples, respectively. On average, the Ct values obtained from saliva as compared to NPS were higher by 2.76. This study demonstrates that saliva performs comparably to NPS for the detection of SARS-CoV-2. Saliva was simple to collect, did not require transport media, and could be tested with equipment readily available at most laboratories. The use of saliva as an acceptable alternative to NPS could support the use of widespread surveillance testing for SARS-CoV-2.

1. Introduction

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected over 32 million individuals since December 2019. Containment strategies are predicated on rapid diagnosis [1] with real-time reverse transcription polymerase chain reaction (rRT-PCR) on nasopharyngeal swab (NPS) specimens as the currently accepted gold standard [2]. However, NPS collection is uncomfortable and may result in testing hesitancy [3], specimen retrieval requires a trained healthcare professional wearing personal protective equipment, and the demand for swabs and transport media has led to shortages.
Using saliva avoids the need for swabs and transport media and permits self-collection [4]. Saliva also remains stable for days at various temperatures [5]. However, reported sensitivity of saliva compared to NPS ranges from 69.2% to 97.6% [6,7,8,9,10]. Most studies are small and are of hospitalized patients only, and some have not investigated the possibility that saliva may identify individuals who falsely test negative by NPS. In addition, patient population, severity of symptoms, testing timing relatively to symptom onset, method of saliva collection and testing platform may all affect test performance characteristics. Thus, on-going comparisons are needed. We assessed the performance characteristics of saliva and NPS for the detection of SARS-CoV-2 in three outpatient testing centers.

2. Materials and Methods

2.1. Study Population and Saliva Collection

All adults (age > 18) who presented to three assessment centers in Toronto, Ontario (North York General Hospital, Michael Garron Hospital, or the Occupation Health Clinic at Sunnybrook Health Sciences Centre), Canada, and had an NPS obtained for SARS-CoV-2 testing from 1 May 2020 until 10 August 2020 were asked to provide as much saliva as they could produce, up to a maximum of 5 mL, into a sterile container that was stored at 4 °C until tested (see Supplementary Material). Self-collection procedures are outlined in the supplementary methods. Once NPS swab results were available, all those positive for SARS-CoV-2 and a random sample of negatives from the same day (ratio of negative to positive 10:1) were selected and matched saliva specimens identified and tested. Clinical information was abstracted by chart review. This study was deemed to be a quality improvement project by the Research Ethics Board of participating hospitals.

2.2. SARS-CoV-2 RNA Detection

NPS samples were tested at Shared Hospital Laboratory (Toronto, ON, Canada). Samples were collected in either universal transport media (UTM; Copan, Italy) or Liquid Amies solution (Eswab collection, Copan, Italy) and nucleic acids using Promega Maxwell HT Viral TNA Kit. Samples were extracted and prepared for PCR using the EP motion 5075 liquid handler (Eppendorf, Mississauga, ON, Canada) with 250 uL of patient material being extracted using the TNA viral extraction kit (Promega) with a final elution volume of 30 µL. Detection of SARS-CoV-2 E-gene and 5′-UTR was performed on the CFX96 Touch Real-time PCR detection system (BioRad, Canada) [11,12]. Alternatively, samples were extracted, and nucleic acid amplification was performed on the BD MAXTM system using the ExK TNA-2 strip (Becton, Dickinson, ND, USA).
Saliva samples were tested at Dynacare Laboratory (Brampton, ON, Canada) using the Roche cobas® 6800 analyzer and Roche cobas® SARS-CoV-2 assay (Hoffmann-La Roche Limited, Mississauga, ON, Canada). All positive samples and a random subset of 23 negative samples were also tested using a second method (see Supplementary Materials).
Prior to testing, saliva samples were equilibrated to room-temperature and vortexed for five seconds to homogenize the sample and disrupt mucus clots. A 600 μL aliquot of each specimen was transferred to a 13 × 75 mm Starstedt tube containing 300 μL of cobas omni Lysis Reagent. In cases where the samples had less than 600 μL volume, 600 μL of cobas omni Lysis Reagent were added directly into the primary sample tube to capture any SARS-CoV-2 RNA potentially present. Samples were vortexed for five seconds and incubated at room-temperature for 10 min in the cobas omni Lysis Reagent, followed by centrifugation at 2500 RCF for 15 min at room-temperature. The samples were loaded onto the Roche cobas 6800 analyzer for testing. The cobas® SARS-CoV-2 test provides fully automated sample preparation (nucleic acid extraction and amplification) and RT-PCR-based qualitative detection of SARS-CoV-2 RNA through amplification of two genomic target regions, ORF1ab and E gene. An armored RNA internal control is added to all samples to validate each reaction. Automated data management software assigns test results for all tests. This test is Health Canada approved for in vitro diagnostic use. Staff who performed saliva testing were blinded to the matched NPS result.
Stability of SARS-CoV-2 in various environmental conditions was assessed using saliva samples collected from nine volunteers. Residual SARS-CoV-2 positive patient sample was spiked into donor saliva and stored for up to seven days at room temperature, 2–8 °C, and −20 °C. Testing occurred at baseline and at days three and seven using the Roche cobas SARS-CoV-2 assay.
The limit of detection for SARS-CoV-2 RNA was determined for the Roche cobas SARS-CoV-2 assay by diluting a 4000 copies/mL positive control (Proceedx Standard, Mississauga, Ontario, Canada Microbix) into pooled negative saliva sample matrix to concentrations of 125, 62.5, 31.25, and 15.63 copies/mL. All dilutions were tested in replicates of 20.

2.3. Supplemental SARS-CoV-2 Testing of Positive Saliva Samples with Paired Negative NPS

All positive samples and a random subset of 23 negative samples were also tested using a second method different than Roche cobas SARS-CoV-2 assay used for primary characterization. This method involved initial offboard virus inactivation by combining 200 μL of each patient saliva sample with 250 μL of lysis buffer master mix, containing TNA lysis buffer (Omega Bio-tek, Inc., Norcoss, GA, USA), Carrier RNA (Omega Bio-tek) and MS2 phage internal control (Thermo Fisher, Waltham, MA, USA). RNA was extracted using MagBind Viral RNA Xpress kit (Omega Bio-tek) on Hamilton Microlab STARlets (Hamilton Company, Reno, NV, USA). A 10 μL aliquot of RNA was added in a 15 μL reaction using TaqPath COVID-19 Combo Kit (Thermo Fisher Scientific) on real-time PCR systems (Applied Biosystems 7500 Fast or QuantStudio 6); one cycle at 25 °C for two minutes, one cycle at 53 °C for 10 min, one cycle at 95 °C for two minutes, 40 cycles at 95 °C for three seconds and 60 °C for 30 s. A sample was defined as positive if the viral genome was detected at threshold cycle (Ct) values of <37 and as negative at Ct values ≥37.

2.4. Statistical Analysis

Descriptive statistics were presented as means or medians as appropriate for continuous variables and proportions for categorical variables. The kappa coefficient was used to estimate the agreement between NPS and saliva SARS-CoV-2 detection results and a paired t-test was used to compare the cycle threshold values. The sensitivity for NPS and saliva were calculated using the total number of positive specimens by either test as the reference standard. All analyses were performed using R, version 4.0.0 (R statistical computing).

3. Results

Overall, 432 paired saliva/NPS patient samples were tested and included in final analysis: 253 (59%), 108 (25%), and 71 (16%) from sites A, B, and C, respectively. Ten (2.3%) of the saliva specimens were highly mucoid; and 54 (13%) were small volume (<0.5 mL) requiring the addition of lysis buffer to a total volume of at least 0.5 mL for testing. Thirty-eight (8.80%) were “invalid” on initial testing, with three (0.69%) yielding repeated invalid results on re-testing. These three repeatedly invalid samples were excluded from final analysis. All invalid results obtained in this study were due to clot errors detected on the Roche cobas 6800 during sample aspiration. The median time from saliva collection to testing was six days (interquartile range (IQR) 5 to 8).
All patients were tested at out-patient assessment centers. Testing characteristics were available from site A only for 236 (54.5%) patients who provided a saliva specimen. The median age of persons with COVID-19 was 42 (IQR 30 to 54), 134 (56.8%) were female, 81 (34.3%) were asymptomatic, and in those with symptoms the median time from symptom onset to testing was 4 days (IQR 2–7 days). Only one individual later required hospitalization.
Seven of 432 samples were discordant between the two sample types, with four individuals testing positive only by NPS and three by saliva only (Table 1 and Table 2), and an observed agreement was 98.4% (kappa coefficient = 0.91, 95% CI 0.82–0.96).
The sensitivity of NPS was 0.93 (95% CI 0.81–0.99) and that of saliva 0.91 (95% CI 0.79–0.98). The rRT-PCR cycle threshold results for concordantly positive NPS and saliva specimens were available for 33/39 pairs (Figure 1). Cycle threshold values obtained from saliva were higher than those from NPS (median difference in Ct 2.76; 95% CI 0.36–5.15, p = 0.03). The limit of detection for SARS-CoV-2 in saliva was 31.25 copies/mL using the Roche cobas SARS-CoV-2 assay.
Viral RNA stability was evaluated by storing spiked donor saliva samples at different temperatures (4 °C, room temperature, and −20 °C) and testing at days 3 and 7. There was no significant difference in Ct values for the ORF1 or E genes in samples tested on day 3 and day 7 compared to initial inoculation (day 0) for samples stored at room temperature or at 4 °C. The median differences for Ct for E gene at room temperature and 4 °C between day 7 and day 0 was −0.3 and −0.8, p = 1.0 and 0.64, respectively (Table 3). Samples stored at −20 °C had higher Ct values on day 7 compared to day 0 (median 1.4 higher, range −0.2 to +3.0, p = 0.01).

4. Discussion

In our out-patient assessment centers, using saliva to diagnose COVID-19 compared favorably to NPS. Saliva was simple to self-collect, needed minimal instruction, avoided the presence of a healthcare professional, and was stable for seven days.
We found that the sensitivity for detecting SARS-CoV-2 in saliva is 91% (95% CI 79–98%). This is comparable to a recent meta-analysis which reported a pooled sensitivity of 0.91 (95% CI 0.80–0.99) [13], and not different from NPS. The limit of detection for SARS-CoV-2 in saliva was also consistent with NPS-tested samples as reported by Roche (31.25 copies/mL and 25–32 copies/mL for saliva and NPS, respectively). Although an increased average Ct value was observed in saliva compared to NPS, this difference was not significant. Interestingly, decreased Ct was observed in some donor samples during sample stability studies, suggesting a paradoxical increase in SARS-CoV-2 RNA. This effect has been observed in other evaluations of SARS-CoV-2 stability in saliva and is thought to be due to assay variability or possibly even virus replication in residual host cells within the sample [5]; however, this effect was not explored further as part of this study.
Many studies are predicated on the assumption that the NPS is the reference standard, which is imperfect given the challenges in obtaining a correct specimen and the possible lower viral loads present in the nasopharynx [9]. As such, a strength of this study, beyond the number of positive specimens tested, is the inclusion of a large number of individuals who tested negative by NPS. Using this approach, we were able to demonstrate that SARS-CoV-2 is detectable in patients who tested negative in a matched NPS sample. The somewhat higher Ct values for saliva compared to NPS in our study is consistent with the somewhat lower sensitivity of saliva compared to NPS later in illness when viral loads in the airway are lower.
Several studies have demonstrated the efficacy of testing saliva for the detection of SARS-CoV-2, however the majority of these used matched samples from hospitalized patients [9,14,15,16], or use commercial saliva collection kits with transport media [8]. Variability in the performance characteristics of saliva may be explained by differing intervals for patient sampling between studies. Extended duration between the onset of symptoms and testing saliva can negatively impact detection rates [17]. However, recent findings by Wyllie and colleagues demonstrated a higher percentage of positive saliva samples compared to NPS in a hospitalized cohort [9]. Differing testing methods and sample preparation strategies are likely to contribute to variable performance [14,18], while individuals presenting to an outpatient setting often differ from inpatients with less severe symptoms that can translate into lower viral loads [19]. Our study population is representative of outpatients with almost 30% being asymptomatic. However, there is still a lack of consensus that disease severity correlates with viral load as similar studies comparing symptomatic and asymptomatic patients have demonstrated similar Ct values [20,21]. Patient demographic or clinical presentation at the time of sample collection did not appear to impact test outcome between sample types in this study.
A potential drawback of testing saliva is the differing viscosity and consistency of the specimens received by the microbiology laboratory. Samples with insufficient volume were frequently encountered, though this did not appear to impact sensitivity. Preparation of saliva was also more laborious compared to NPS, with highly mucoid samples requiring additional homogenizing to prevent instrument clotting failures. Although the invalid rate for saliva was improved from 8.80% to 0.69% through centrifugation, this is still much higher than the invalid rate for NPS collected samples, which is 0.04%. Importantly, one discordant saliva sample that was falsely-negative compared to the matched NPS-collected sample was noted to be highly mucoid and challenging to pipette, requiring vigorous vortexing.
There are limitations of the study that merit emphasis. First, not all individuals who tested positive by NPS agreed to submit a saliva specimen for testing. This may simply be because submission of saliva was voluntary, but we cannot exclude the possibility that some participants may not have been able to produce saliva on demand. Second, our sample cohort was adults, and our results may not apply to children. Third, in our low prevalence setting, false positive results may occur. However, testing for two targets and the low cycle threshold values observed make it likely that all were truly positive. Fourth, different assays were used for testing of saliva compared to NPS, which is a potential cause of the differences in Ct values.

5. Conclusions

In conclusion, saliva is an acceptable alternative to an NPS for the detection of SARS-CoV-2 in adult outpatients. The simple and non-invasive self-collection procedure avoids the need for a swab, reduces exposure to healthcare workers, and can be self-collected in any environment. This has the potential to be used in settings such as workplace screening, where repeated testing may be needed, or in outbreak settings where testing of large numbers of people simultaneously is warranted.

Supplementary Materials

Supplementary materials can be found at

Author Contributions

Conceptualization, L.W.G. and R.K.; methodology, L.W.G. and R.K.; formal analysis, C.K., J.Z., and A.M.; investigation E.S., J.Z., C.V., J.M., H.R., M.D., J.E.P., K.V., and L.F.; resources, C.V., K.K., L.G., and R.K.; data curation, M.A.S., J.Z., and C.K.; writing—original draft preparation, C.K. and J.Z.; writing—review and editing, R.K., L.G., K.K., C.V., and A.M.; supervision, R.K.; project administration, R.K. and L.G. All authors have read and agreed to the published version of the manuscript.


This research received no external funding. C.K. receives support from the Eliot Phillipson Clinician Scientist Training Program from the Faculty of Medicine at the University of Toronto.


We thank the clinical care teams at the outpatient assessment centers at Michael Garron Hospital and North York General Hospital Branson site and the Occupational Health Department at Sunnybrook Health Sciences Centre for their support.

Conflicts of Interest

C.K., J.Z., J.M., J.E.P., K.V., L.F., E.S., C.V., K.K., A.M., and R.M. have no conflicts of interest to declare. M.A.S., H.R., M.D., and L.W.G. are employees of Dynacare (a subsidiary of Laboratory Corporation of America), which is a private, for-profit laboratory that offers diagnostic testing services to government and non-government clients. While the individuals noted herein will not personally benefit from this submission, the company could benefit financially if the test is commercially marketed.


  1. Santosh, T.S.; Parmar, R.; Anand, H.; Srikanth, K.; Saritha, M. A Review of Salivary Diagnostics and Its Potential Implication in Detection of Covid-19. Cureus 2020, 12, e7708. [Google Scholar] [CrossRef][Green Version]
  2. Hong, K.H.; Lee, S.W.; Kim, T.S.; Huh, H.J.; Lee, J.; Kim, S.Y.; Park, J.-S.; Kim, G.J.; Sung, H.; Roh, K.H.; et al. Guidelines for Laboratory Diagnosis of Coronavirus Disease 2019 (COVID-19) in Korea. Ann. Lab. Med. 2020, 40, 351–360. [Google Scholar] [CrossRef] [PubMed]
  3. Azzi, L.; Carcano, G.; Gianfagna, F.; Grossi, P.; Gasperina, D.D.; Genoni, A.; Fasano, M.; Sessa, F.; Tettamanti, L.; Carinci, F.; et al. Saliva is a reliable tool to detect SARS-CoV-2. J. Infect. 2020, 81, e45–e50. [Google Scholar] [CrossRef]
  4. Khurshid, Z.; Zohaib, S.; Joshi, C.; Moin, S.F.; Zafar, M.S.; Speicher, D.J. Saliva as a non-invasive sample for the detection of SARS-CoV-2: A systematic review. medRxiv 2020. [Google Scholar] [CrossRef]
  5. Ott, I.; Simões, M.S.; Watkins, A.; Boot, M.; Kalinich, C.C.; Harden, C.A.; Wyllie, A.L.; Casanovas-Massana, A.; Moore, A.J.; Muenker, C.; et al. Simply saliva: Stability of SARS-CoV-2 detection negates the need for expensive collection devices. medRxiv 2020. [Google Scholar] [CrossRef]
  6. Becker, D.; Sandoval, E.; Amin, A.; De Hoff, P.; Diets, A.; Leonetti, N.; Lim, Y.W.; Elliott, C.; Laurent, L.; Grzymski, J.; et al. Saliva is less sensitive than nasopharyngeal swabs for COVID-19 detection in the community setting. medRxiv 2020. [Google Scholar] [CrossRef]
  7. Williams, E.; Bond, K.; Zhang, B.; Putland, M.; Williamson, D. Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2. J. Clin. Microbiol. 2020, 58. [Google Scholar] [CrossRef] [PubMed][Green Version]
  8. Caulley, L.; Corsten, M.; Eapen, L.; Whelan, B.J.; Angel, J.B.; Antonation, M.K.; Bastien, N.; Poliquin, G.; Johnson-Obaseki, S. Salivary Detection of COVID-19. Ann. Intern. Med. 2020. [Google Scholar] [CrossRef] [PubMed]
  9. Wyllie, A.L.; Fournier, J.; Casanovas-Massana, A.; Campbell, M.; Tokuyama, M.; Vijayakumar, P.; Petrone, M.E. Faculty Opinions recommendation of Saliva is more sensitive for SARS-CoV-2 detection in COVID-19 patients than nasopharyngeal swabs. Faculty Opinions Post-Publication Peer Review of the Biomedical Literature 2020. Medrxiv 2020. [Google Scholar] [CrossRef][Green Version]
  10. Peeters, E.; Singh, S.K.D.A.; Vandesompele, J.; Mestdagh, P.; Hutse, V.; Arbyn, M. Rapid systematic review of the sensitivity of SARS-CoV-2 molecular testing on saliva compared to nasopharyngeal swabs. medRxiv 2020. [Google Scholar] [CrossRef]
  11. Corman, V.M.; Landt, O.; Kaiser, M.; Molenkamp, R.; Meijer, A.; Chu, D.K.; Bleicker, T.; Brünink, S.; Schneider, J.; Schmidt, M.L.; et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Eurosurveillance 2020, 25, 2000045. [Google Scholar] [CrossRef] [PubMed][Green Version]
  12. Vermeiren, C.; Marchand-Senécal, X.; Sheldrake, E.; Bulir, D.; Smieja, M.; Chong, S.; Forbes, J.D.; Katz, K. Comparison of Copan ESwab and FLOQSwab for COVID-19 Diagnosis: Working around a Supply Shortage. J. Clin. Microbiol. 2020, 58. [Google Scholar] [CrossRef] [PubMed][Green Version]
  13. Czumbel, L.M.; Kiss, S.; Farkas, N.; Mandel, I.; Hegyi, A.; Ákos, N.; Lohinai, Z.; Szakács, Z.; Hegyi, P.; Steward, M.C.; et al. Saliva as a Candidate for COVID-19 Diagnostic Testing: A Meta-Analysis. Front. Med. 2020, 7, 465. [Google Scholar] [CrossRef] [PubMed]
  14. Rao, M.; Rashid, F.A.; Sabri, F.S.A.H.; Jamil, N.N.; Zain, R.; Hashim, R.; Amran, F.; Kok, H.T.; Samad, A.A.; Ahmad, N. Comparing nasopharyngeal swab and early morning saliva for the identification of SARS-CoV-2. Clin. Infect. Dis. 2020. [Google Scholar] [CrossRef]
  15. Iwasaki, S.; Fujisawa, S.; Nakakubo, S.; Kamada, K.; Yamashita, Y.; Fukumoto, T.; Sato, K.; Oguri, S.; Taki, K.; Senjo, H.; et al. Comparison of SARS-CoV-2 detection in nasopharyngeal swab and saliva. J. Infect. 2020, 81, e145–e147. [Google Scholar] [CrossRef]
  16. To, K.K.-W.; Tsang, O.T.-Y.; Yip, C.C.-Y.; Chan, K.-H.; Wu, T.-C.; Chan, J.M.-C.; Leung, W.-S.; Chik, T.S.-H.; Choi, C.Y.-C.; Kandamby, D.H.; et al. Consistent Detection of 2019 Novel Coronavirus in Saliva. Clin. Infect. Dis. 2020, 71, 841–843. [Google Scholar] [CrossRef][Green Version]
  17. Jamal, A.J.; Mozafarihashjin, M.; Coomes, E.; Powis, J.; Li, A.X.; Paterson, A.; Anceva-Sami, S.; Barati, S.; Crowl, G.; Faheem, A.; et al. Sensitivity of Nasopharyngeal Swabs and Saliva for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Clin. Infect. Dis. 2020, 25, ciaa848. [Google Scholar] [CrossRef]
  18. Wyllie, A.L.; Fournier, J.; Casanovas-Massana, A.; Campbell, M.; Tokuyama, M.; Vijayakumar, P.; Warren, J.L.; Geng, B.; Muenker, M.C.; Moore, A.J.; et al. Saliva or Nasopharyngeal Swab Specimens for Detection of SARS-CoV-2. N. Engl. J. Med. 2020, 383, 1283–1286. [Google Scholar] [CrossRef]
  19. Liu, Y.; Yan, L.-M.; Wan, L.; Xiang, T.-X.; Le, A.; Liu, J.-M.; Peiris, M.; Poon, L.L.M.; Zhang, W. Viral dynamics in mild and severe cases of COVID-19. Lancet Infect. Dis. 2020, 20, 656–657. [Google Scholar] [CrossRef][Green Version]
  20. Long, Q.; Tang, X.-J.; Shi, Q.-L.; Li, Q.; Deng, H.-J.; Yuan, J.; Hu, J.-L.; Xu, W.; Zhang, Y.; Lv, F.-J.; et al. Clinical and immunological assessment of asymptomatic SARS-CoV-2 infections. Nat. Med. 2020, 26, 1200–1204. [Google Scholar] [CrossRef]
  21. Wang, D.; Hu, B.; Hu, C.; Zhu, F.; Liu, X.; Zhang, J.; Wang, B.; Xiang, H.; Cheng, Z.; Xiong, Y.; et al. Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus–Infected Pneumonia in Wuhan, China. JAMA 2020, 323, 1061. [Google Scholar] [CrossRef] [PubMed]
Figure 1. Cycle threshold values for the E gene target of SARS-CoV-2 RNA retrieved from individuals who were concordantly positive by nasopharyngeal swab and saliva.
Figure 1. Cycle threshold values for the E gene target of SARS-CoV-2 RNA retrieved from individuals who were concordantly positive by nasopharyngeal swab and saliva.
Viruses 12 01314 g001
Table 1. Results of SARS-CoV-2 detection in paired nasopharyngeal swab and saliva specimens from individuals who presented to an outpatient testing center.
Table 1. Results of SARS-CoV-2 detection in paired nasopharyngeal swab and saliva specimens from individuals who presented to an outpatient testing center.
Saliva SampleNasopharyngeal SwabTotal
Table 2. Discordant results of matched nasopharyngeal swab (NPS) and saliva sample pairs.
Table 2. Discordant results of matched nasopharyngeal swab (NPS) and saliva sample pairs.
ResultsNPS CT ValuesSaliva CT Values
NPSSalivaTarget 1 *Target 2 Internal ControlTarget 1 Target 2 §Internal Control
Sample 1positivenegative27.026.825.1NDND35.3
Sample 2positivenegative39.238.926.1NDND35.0
Sample 3positivenegative31.733.521.2NDND34.5
Sample 4positivenegative31.931.820.7NDND22.5
Sample 5negativepositiveNDND21.5ND37.036.8
Sample 6negativepositiveNDND24.229.431.738.6
Sample 7negativepositiveNDND27.630.131.336.2
* Target 1: E-gene; Target 2: 5′-UTR; Target 1: ORF1ab; § Target 2: E gene; ND: not detected.
Table 3. Stability of SARS-CoV-2 in spiked saliva samples.
Table 3. Stability of SARS-CoV-2 in spiked saliva samples.
Room Temperature (18–24 ℃)
Saliva SampleDay 0 (Ct)Day 3 (Ct)Day7 (Ct)Day 3 Δ (Ct)Day 7 Δ (Ct)
ORF1a/bE geneInternal ControlORF1a/bE geneInternal ControlORF1a/bE geneInternal ControlORF1a/bE geneORF1a/bE gene
Refrigerated (2–8 ℃)
Saliva SampleDay 0Day3Day7Day 3 ΔDay 7 Δ
ORF1a/bE geneInternal ControlORF1a/bE geneInternal ControlORF1a/bE geneInternal ControlORF1a/bE geneORF1a/bE gene
Frozen (−20 ℃)
Day 0
Day3Day7Day 3 ΔDay 7 Δ
E geneInternal ControlORF1a/bE geneInternal ControlORF1a/bE geneInternal ControlORF1a/bE geneORF1a/bE gene
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Kandel, C.; Zheng, J.; McCready, J.; Serbanescu, M.A.; Racher, H.; Desaulnier, M.; Powis, J.E.; Vojdani, K.; Finlay, L.; Sheldrake, E.; Vermeiren, C.; Katz, K.; McGeer, A.; Kozak, R.; Goneau, L.W. Detection of SARS-CoV-2 from Saliva as Compared to Nasopharyngeal Swabs in Outpatients. Viruses 2020, 12, 1314.

AMA Style

Kandel C, Zheng J, McCready J, Serbanescu MA, Racher H, Desaulnier M, Powis JE, Vojdani K, Finlay L, Sheldrake E, Vermeiren C, Katz K, McGeer A, Kozak R, Goneau LW. Detection of SARS-CoV-2 from Saliva as Compared to Nasopharyngeal Swabs in Outpatients. Viruses. 2020; 12(11):1314.

Chicago/Turabian Style

Kandel, Christopher, Jennifer Zheng, Janine McCready, Mihaela Anca Serbanescu, Hilary Racher, Melissa Desaulnier, Jeff E Powis, Kyle Vojdani, Laura Finlay, Elena Sheldrake, Christie Vermeiren, Kevin Katz, Allison McGeer, Robert Kozak, and Lee W Goneau. 2020. "Detection of SARS-CoV-2 from Saliva as Compared to Nasopharyngeal Swabs in Outpatients" Viruses 12, no. 11: 1314.

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop