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Article

Determination of Genetically Identical Strains of Four Honeybee Viruses in Bumblebee Positive Samples

1
Institute of Microbiology and Parasitology, Virology Unit, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
2
Institute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
3
National Institute of Biology, Večna pot 111, 1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(11), 1310; https://doi.org/10.3390/v12111310
Received: 14 October 2020 / Revised: 9 November 2020 / Accepted: 12 November 2020 / Published: 16 November 2020
(This article belongs to the Special Issue State-of-the-Art Animal Viruses Research in Slovenia)
In recent years, there has been growing evidence that certain types of honeybee viruses could be transmitted between different pollinators. Within a voluntary monitoring programme, 180 honeybee samples (Apis mellifera carnica) were collected from affected apiaries between 2007 and 2018. Also from August 2017 to August 2018, a total 148 samples of healthy bumblebees (Bombus lapidarius, B. pascuorum, B. terrestris, B. lucorum, B. hortorum, B. sylvarum, B. humilis) were collected at four different locations in Slovenia, and all samples were tested by using RT-PCR methods for six honeybee viruses. Direct sequencing of a total 158 positive samples (acute bee paralysis virus (ABPV n = 33), black queen cell virus (BQCV n = 75), sacbrood bee virus (SBV n = 25) and Lake Sinai virus (LSV n = 25)) was performed from obtained RT-PCR products. The genetic comparison of identified positive samples of bumblebees and detected honeybee field strains of ABPV, BQCV, SBV, and LSV demonstrated 98.74% to 100% nucleotide identity between both species. This study not only provides evidence that honeybees and bumblebees are infected with genetically identical or closely related viral strains of four endemically present honeybee viruses but also detected a high diversity of circulating strains in bumblebees, similar as was observed among honeybees. Important new genetic data for endemic strains circulating in honeybees and bumblebees in Slovenia are presented. View Full-Text
Keywords: honeybees; bumblebees; viruses; sequencing; transmission; epidemiology honeybees; bumblebees; viruses; sequencing; transmission; epidemiology
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MDPI and ACS Style

Toplak, I.; Šimenc, L.; Pislak Ocepek, M.; Bevk, D. Determination of Genetically Identical Strains of Four Honeybee Viruses in Bumblebee Positive Samples. Viruses 2020, 12, 1310. https://doi.org/10.3390/v12111310

AMA Style

Toplak I, Šimenc L, Pislak Ocepek M, Bevk D. Determination of Genetically Identical Strains of Four Honeybee Viruses in Bumblebee Positive Samples. Viruses. 2020; 12(11):1310. https://doi.org/10.3390/v12111310

Chicago/Turabian Style

Toplak, Ivan, Laura Šimenc, Metka Pislak Ocepek, and Danilo Bevk. 2020. "Determination of Genetically Identical Strains of Four Honeybee Viruses in Bumblebee Positive Samples" Viruses 12, no. 11: 1310. https://doi.org/10.3390/v12111310

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