A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (Capreolus capreolus) and Red Deer (Cervus elaphus): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples
2.2. Histopathology
2.3. PCR and Sanger Sequencing of the Partial L1 Gene
2.4. Whole-Genome Sequencing
2.5. Bioinformatic Analysis of NGS Data
2.6. Phylogenetic Analysis
2.7. Data Availability Statement
3. Results
3.1. Gross Pathology and Histopathology
3.2. PCR and Sanger Sequencing of the Partial L1 Gene
3.3. Whole-Genome Sequencing and Genome Analysis
3.4. Phylogenetic Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Animal Species/Year/Month of Harvesting | Sex | Age (in Years) |
---|---|---|
Roe deer/2014/August | Male | 2 |
Roe deer/2014/December | Male | 3 |
Roe deer/2015/October | Female | 6 |
Roe deer/2016/May | Male | 6 |
Roe deer/2016/May | Male | 3 |
Roe deer/2016/December | Female | 6 |
Red deer/2016/September | Female | 1 |
PV Strain | Total Reads | Mapped Reads | Mean Read Length |
---|---|---|---|
CcaPV1-1 (31-16) | 197,354 | 312 | 326 |
CcaPV1-1 (32-16) | 229,894 | 737 | 302 |
CcaPV1-1 (103-16) | 206,940 | 1519 | 279 |
CcaPV1-1 (63-15) | 213,479 | 241 | 309 |
CcaPV1-1 (37-14) | 254,725 | 380 | 292 |
CcaPV1-2 (84-14) | 317,345 | 636 | 278 |
CePV1v-1 (64-16) | 2,867,278 | 10,010 | 282 |
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Kmetec, J.; Kuhar, U.; Fajfar, A.G.; Vengušt, D.Ž.; Vengušt, G. A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (Capreolus capreolus) and Red Deer (Cervus elaphus): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses. Viruses 2020, 12, 1001. https://doi.org/10.3390/v12091001
Kmetec J, Kuhar U, Fajfar AG, Vengušt DŽ, Vengušt G. A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (Capreolus capreolus) and Red Deer (Cervus elaphus): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses. Viruses. 2020; 12(9):1001. https://doi.org/10.3390/v12091001
Chicago/Turabian StyleKmetec, Jernej, Urška Kuhar, Aleksandra Grilc Fajfar, Diana Žele Vengušt, and Gorazd Vengušt. 2020. "A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (Capreolus capreolus) and Red Deer (Cervus elaphus): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses" Viruses 12, no. 9: 1001. https://doi.org/10.3390/v12091001
APA StyleKmetec, J., Kuhar, U., Fajfar, A. G., Vengušt, D. Ž., & Vengušt, G. (2020). A Comprehensive Study of Cutaneous Fibropapillomatosis in Free-Ranging Roe Deer (Capreolus capreolus) and Red Deer (Cervus elaphus): from Clinical Manifestations to Whole-Genome Sequencing of Papillomaviruses. Viruses, 12(9), 1001. https://doi.org/10.3390/v12091001