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Open AccessArticle

Characterization of Novel Lytic Bacteriophages of Achromobacter marplantensis Isolated from a Pneumonia Patient

1
Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, Victoria 3083, Australia
2
Department of Microbiology, Royal Melbourne Hospital, Victoria 3000, Australia
3
Department of Pharmacy and Biomedical Science, La Trobe Institute for Molecular Science, La Trobe University, Bendigo, Victoria 3552, Australia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(10), 1138; https://doi.org/10.3390/v12101138
Received: 20 August 2020 / Revised: 2 October 2020 / Accepted: 6 October 2020 / Published: 8 October 2020
(This article belongs to the Section Bacterial Viruses)
Achromobacter spp. are becoming increasingly associated with lung infections in patients suffering from cystic fibrosis (CF). A. marplatensis, which is closely related to A. xylosoxidans, has been isolated from the lungs of CF patients and other human infections. This article describes the isolation, morphology and characterization of two lytic bacteriophages specific for an A. marplatensis strain isolated from a pneumonia patient. This host strain was the causal agent of hospital acquired pneumonia–the first clinical report of such an occurrence. Full genome sequencing revealed bacteriophage genomes ranging in size from 45901 to 46,328 bp. Transmission electron microscopy revealed that the two bacteriophages AMA1 and AMA2 belonged to the Siphoviridae family. Host range analysis showed that their host range did not extend to A. xylosoxidans. The possibility exists for future testing of such bacteriophages in the control of Achromobacter infections such as those seen in CF and other infections of the lungs. The incidence of antibiotic resistance in this genus highlights the importance of seeking adjuncts and alternatives in CF and other lung infections. View Full-Text
Keywords: bacteriophage; Achromobacter marplatensis; cystic fibrosis; pneumonia bacteriophage; Achromobacter marplatensis; cystic fibrosis; pneumonia
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MDPI and ACS Style

Chan, H.T.; Ku, H.; Low, Y.P.; Batinovic, S.; Kabwe, M.; Petrovski, S.; Tucci, J. Characterization of Novel Lytic Bacteriophages of Achromobacter marplantensis Isolated from a Pneumonia Patient. Viruses 2020, 12, 1138.

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