A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sequence Sources
2.2. Sequence Analysis
2.3. 3D Modeling
2.4. Planar Lipid Bilayer Experiments
2.5. Channel Protein Synthesis
2.6. Ion Channel Recordings, Data Analysis, and Statistics
3. Results and Discussion
3.1. Putative K+ Channels
3.2. The TetV-1 Protein Has K+ Channel Function
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Source of Putative Channel Protein (NCBI Accession #) | Most Similar Non-Virus Hit, Organism Max Score, Total Score, Query Cover, E-Value, aa Identity, Accession # | Most Similar Virus Hit, Virus Max Score, Total Score, Query Cover, E-Value, aa Identity, Accession # |
---|---|---|
RhiV-SA1 (MT926120) | Oleiphilus messinensis (Proteobacteria) 49.7, 49.7, 72%, 2 × 10−4, 36%, WP_087462234.1 | Acanthocystis turfacea chlorella virus mid_1.1 ATCV-1 (Chlorovirus) 43.9, 43.9, 46%, 4 × 10−5, 34%, QLC35876.1 |
FloV-SA1 (MT926122) | Actinomycetales bacterium (Actinobacteria) 77.8, 77.8, 88%, 3 × 10−15, 38%, PQM59864.1 | Aureococcus anophagefferens virus (Aav) 58.2, 58.2, 90%, 1 × 10−9, 33%, YP_009052228.1 |
CsV-KB1 (MT926121) | Actinomycetales bacterium (Actinobacteria) 93.2, 93.2, 86%, 8 × 10−21, 36%, PQM59864.1 | Organic Lake phycodnavirus 2 (OLPV2) 82.8, 82.8, 76%, 4 × 10−19, 39%, ADX06223.1 |
TetV-1 (AUF82121.1) | Streptomyces laurentii (Actinobacteria) 55.5, 55.5, 67%, 4 × 10−7, 42%, BAU83004.1 | Ostreococcus tauri virus RT-201 40.8, 40.8, 61%, 4 × 10−4, 41%, AFC34969.1 |
Source of Putative Channel Protein | nc/na in DPhPC | nc/na in DPhPG |
---|---|---|
RhiV-SA1 | 0/3 | not tested |
FloV-SA1 | 0/3 | 0/1 |
CsV-KB1 | 0/3 | not tested |
TetV-1 | 39/39 | not tested |
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Kukovetz, K.; Hertel, B.; Schvarcz, C.R.; Saponaro, A.; Manthey, M.; Burk, U.; Greiner, T.; Steward, G.F.; Van Etten, J.L.; Moroni, A.; et al. A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae. Viruses 2020, 12, 1107. https://doi.org/10.3390/v12101107
Kukovetz K, Hertel B, Schvarcz CR, Saponaro A, Manthey M, Burk U, Greiner T, Steward GF, Van Etten JL, Moroni A, et al. A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae. Viruses. 2020; 12(10):1107. https://doi.org/10.3390/v12101107
Chicago/Turabian StyleKukovetz, Kerri, Brigitte Hertel, Christopher R. Schvarcz, Andrea Saponaro, Mirja Manthey, Ulrike Burk, Timo Greiner, Grieg F. Steward, James L. Van Etten, Anna Moroni, and et al. 2020. "A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae" Viruses 12, no. 10: 1107. https://doi.org/10.3390/v12101107