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A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae

Membrane Biophysics, Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany
Department of Oceanography, Daniel K. Inouye Center for Microbial Oceanography: Research and Education, University of Hawai’i at Mānoa, 1950 East-West Road, Honolulu, HI 96822, USA
Department of Biosciences and CNR IBF-Mi, Università degli Studi di Milano, Via Giovanni Celoria 26, 20133 Milano, Italy
University Clinic for Psychiatry and Psychotherapy, Brandenburg Medical School, Immanuel Klinik Rüdersdorf, Seebad 82/83, 15562 Rüdersdorf, Germany
Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska Lincoln, Lincoln, NE 68583-0900, USA
Institute of Biophysics, Consiglio Nazionale delle Ricerche, Via Celoria 26, 20133 Milan, Italy
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(10), 1107;
Received: 7 September 2020 / Revised: 25 September 2020 / Accepted: 25 September 2020 / Published: 29 September 2020
(This article belongs to the Collection Unconventional Viruses)
Potassium ion (K+) channels have been observed in diverse viruses that infect eukaryotic marine and freshwater algae. However, experimental evidence for functional K+ channels among these alga-infecting viruses has thus far been restricted to members of the family Phycodnaviridae, which are large, double-stranded DNA viruses within the phylum Nucleocytoviricota. Recent sequencing projects revealed that alga-infecting members of Mimiviridae, another family within this phylum, may also contain genes encoding K+ channels. Here we examine the structural features and the functional properties of putative K+ channels from four cultivated members of Mimiviridae. While all four proteins contain variations of the conserved selectivity filter sequence of K+ channels, structural prediction algorithms suggest that only two of them have the required number and position of two transmembrane domains that are present in all K+ channels. After in vitro translation and reconstitution of the four proteins in planar lipid bilayers, we confirmed that one of them, a 79 amino acid protein from the virus Tetraselmis virus 1 (TetV-1), forms a functional ion channel with a distinct selectivity for K+ over Na+ and a sensitivity to Ba2+. Thus, virus-encoded K+ channels are not limited to Phycodnaviridae but also occur in the members of Mimiviridae. The large sequence diversity among the viral K+ channels implies multiple events of lateral gene transfer. View Full-Text
Keywords: viral K+ channel; Kcv; K+ channel evolution; Phycodnaviridae; Mimiviridae viral K+ channel; Kcv; K+ channel evolution; Phycodnaviridae; Mimiviridae
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MDPI and ACS Style

Kukovetz, K.; Hertel, B.; Schvarcz, C.R.; Saponaro, A.; Manthey, M.; Burk, U.; Greiner, T.; Steward, G.F.; Van Etten, J.L.; Moroni, A.; Thiel, G.; Rauh, O. A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae. Viruses 2020, 12, 1107.

AMA Style

Kukovetz K, Hertel B, Schvarcz CR, Saponaro A, Manthey M, Burk U, Greiner T, Steward GF, Van Etten JL, Moroni A, Thiel G, Rauh O. A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae. Viruses. 2020; 12(10):1107.

Chicago/Turabian Style

Kukovetz, Kerri, Brigitte Hertel, Christopher R. Schvarcz, Andrea Saponaro, Mirja Manthey, Ulrike Burk, Timo Greiner, Grieg F. Steward, James L. Van Etten, Anna Moroni, Gerhard Thiel, and Oliver Rauh. 2020. "A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae" Viruses 12, no. 10: 1107.

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