RNase L Is Involved in Liposaccharide-Induced Lung Inflammation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Antibodies
2.2. Knocking Down of RNase L in Raw 264.7 Cells
2.3. Animal Treatment
2.4. Analysis of RNase L-Mediated rRNA Cleavages in Intact Cells
2.5. Western Blot Analysis
2.6. Histostaining
2.7. Enzyme-Linked Immunosorbent Assay (ELISA)
3. Results
3.1. Deficiency of RNase L Increases Lung Tissue Damage in LPS-Induced ALI
3.2. RNase L Regulates the Expression of Pro-and Anti-Inflammatory Genes in the Lung and Blood upon LPS Stimulation
3.3. RNase L Mediates the TLR4 Signaling Pathway Activated by LPS in Macrophages
3.4. RNase L Function in LPS-Induced Lung Injury May Be Independent of Its Nuclease Activity
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Wei, R.; Chen, G.; Algehainy, N.; Zeng, C.; Liu, C.; Liu, H.; Liu, W.; Stacey, D.; Zhou, A. RNase L Is Involved in Liposaccharide-Induced Lung Inflammation. Viruses 2020, 12, 73. https://doi.org/10.3390/v12010073
Wei R, Chen G, Algehainy N, Zeng C, Liu C, Liu H, Liu W, Stacey D, Zhou A. RNase L Is Involved in Liposaccharide-Induced Lung Inflammation. Viruses. 2020; 12(1):73. https://doi.org/10.3390/v12010073
Chicago/Turabian StyleWei, Ruhan, Guanmin Chen, Naseh Algehainy, Chun Zeng, Chunfang Liu, Hongli Liu, Wendy Liu, Dennis Stacey, and Aimin Zhou. 2020. "RNase L Is Involved in Liposaccharide-Induced Lung Inflammation" Viruses 12, no. 1: 73. https://doi.org/10.3390/v12010073