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Volume 12
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10.3390/v12010046
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Article

Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils

by
Yi-Sheng Sun
,
Zhang-Nv Yang
,
Fang Xu
,
Chen Chen
,
Hang-Jing Lu
,
Jian-Min Jiang
,
Yan-Jun Zhang
,
Han-Ping Zhu
* and
Ping-Ping Yao
*
Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310015, China
*
Authors to whom correspondence should be addressed.
Viruses 2020, 12(1), 46; https://doi.org/10.3390/v12010046
Submission received: 7 November 2019 / Revised: 16 December 2019 / Accepted: 26 December 2019 / Published: 30 December 2019
(This article belongs to the Section Animal Viruses)
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Abstract

Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the two most important pathogens of hand, foot, and mouth disease (HFMD). However, the neuropathogenesis of EV71 and CVA16 has not been elucidated. In our previous study, we established gerbils as a useful model for both EV71 and CVA16 infection. In this work, we used RNA-seq technology to analyze the global gene expression of the brainstem of EV71- and CVA16-infected gerbils. We found that 3434 genes were upregulated while 916 genes were downregulated in EV71-infected gerbils. In CVA16-infected gerbils, 1039 genes were upregulated, and 299 genes were downregulated. We also found significant dysregulation of cytokines, such as IP-10 and CXCL9, in the brainstem of gerbils. The expression levels of 10 of the most upregulated genes were confirmed by real-time RT-PCR, and the upregulated tendency of most genes was in accordance with the differential gene expression (DGE) results. Our work provided global gene expression analysis of virus-infected gerbils and laid a solid foundation for elucidating the neuropathogenesis mechanisms of EV71 and CVA16.
Keywords: enterovirus 71; coxsackievirus 16; gerbils; neuropathogenesis; cytokine dysregulation enterovirus 71; coxsackievirus 16; gerbils; neuropathogenesis; cytokine dysregulation

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MDPI and ACS Style

Sun, Y.-S.; Yang, Z.-N.; Xu, F.; Chen, C.; Lu, H.-J.; Jiang, J.-M.; Zhang, Y.-J.; Zhu, H.-P.; Yao, P.-P. Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils. Viruses 2020, 12, 46. https://doi.org/10.3390/v12010046

AMA Style

Sun Y-S, Yang Z-N, Xu F, Chen C, Lu H-J, Jiang J-M, Zhang Y-J, Zhu H-P, Yao P-P. Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils. Viruses. 2020; 12(1):46. https://doi.org/10.3390/v12010046

Chicago/Turabian Style

Sun, Yi-Sheng, Zhang-Nv Yang, Fang Xu, Chen Chen, Hang-Jing Lu, Jian-Min Jiang, Yan-Jun Zhang, Han-Ping Zhu, and Ping-Ping Yao. 2020. "Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils" Viruses 12, no. 1: 46. https://doi.org/10.3390/v12010046

APA Style

Sun, Y.-S., Yang, Z.-N., Xu, F., Chen, C., Lu, H.-J., Jiang, J.-M., Zhang, Y.-J., Zhu, H.-P., & Yao, P.-P. (2020). Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils. Viruses, 12(1), 46. https://doi.org/10.3390/v12010046

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