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Article

Detailed Characteristics of Tonsillar Tumors with Extrachromosomal or Integrated Form of Human Papillomavirus

1
Department of Genetics and Microbiology, Faculty of Science, Charles University, BIOCEV, Průmyslová 595, 25250 Vestec, Czech Republic
2
Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, BIOCEV, Průmyslová 595, 25250 Vestec, Czech Republic
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(1), 42; https://doi.org/10.3390/v12010042
Received: 27 November 2019 / Revised: 22 December 2019 / Accepted: 26 December 2019 / Published: 30 December 2019
(This article belongs to the Section Animal Viruses)
The human papillomavirus (HPV) integration, the critical step in viral carcinogenesis, most frequently occurs in the E2 gene, which results in its inactivation and in an increase of E6/E7 transcription. However, in a substantial number of tumors, the virus is present in an extrachromosomal form. For those tumors, the transformation mechanisms are not fully elucidated. Here we evaluated the possible mechanism of inactivating the E2 without interruption of the gene, methylation or mutation of the E2 binding sites (E2BSs) in HPV16-positive tonsillar tumors by next-generation and Sanger sequencing. Viral genome status was analyzed by the amplification of papillomavirus oncogene transcripts assay (APOT) and mRNA mapping, and expression of viral oncogenes was performed by qPCR. The methylation of E2BSs was significantly higher in tumors with an integrated, in comparison to extrachromosomal, form of the viral genome. No mutations were detected in the E2BSs. The viral oncogenes were equally expressed in samples with an integrated and extrachromosomal form of the virus. Only the nucleotide variants were identified in the E2 gene. No proposed mechanism of E2 inactivation was confirmed in tonsillar tumors with an extrachromosomal form of the HPV genome. The expression of E6/E7 genes seems to be sufficient to initiate and maintain the carcinogenic process View Full-Text
Keywords: human papillomavirus; E2 binding sites; methylation; genome status human papillomavirus; E2 binding sites; methylation; genome status
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MDPI and ACS Style

Pokrývková, B.; Saláková, M.; Šmahelová, J.; Vojtěchová, Z.; Novosadová, V.; Tachezy, R. Detailed Characteristics of Tonsillar Tumors with Extrachromosomal or Integrated Form of Human Papillomavirus. Viruses 2020, 12, 42. https://doi.org/10.3390/v12010042

AMA Style

Pokrývková B, Saláková M, Šmahelová J, Vojtěchová Z, Novosadová V, Tachezy R. Detailed Characteristics of Tonsillar Tumors with Extrachromosomal or Integrated Form of Human Papillomavirus. Viruses. 2020; 12(1):42. https://doi.org/10.3390/v12010042

Chicago/Turabian Style

Pokrývková, Barbora, Martina Saláková, Jana Šmahelová, Zuzana Vojtěchová, Vendula Novosadová, and Ruth Tachezy. 2020. "Detailed Characteristics of Tonsillar Tumors with Extrachromosomal or Integrated Form of Human Papillomavirus" Viruses 12, no. 1: 42. https://doi.org/10.3390/v12010042

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