Mouse Cytomegalovirus Differentially Exploits Cell Surface Glycosaminoglycans in a Cell Type-Dependent and MCK-2-Independent Manner
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Cells
2.3. Viruses
2.4. Luciferase-Based Infectivity Assays
2.5. Heparin-Binding Assays
2.6. B18 Cell Surface Binding Assays
2.7. qPCR Analysis of Acinar Cell Markers
2.8. MCMV Cell Binding Assays
3. Results
3.1. MCMV Infectivity Is Differentially Influenced by Target Cell Type-Specific GAGs in an MCK-2 Independent Manner
3.2. MCK-2 Is Not Required for the MCMV Infection of Mouse Salivary Gland Acinar Cells (SGACs)
3.3. MCK-2 is Dispensable for MCMV Attachment to the Surface of Fibroblasts and Macrophages
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Pontejo, S.M.; Murphy, P.M. Mouse Cytomegalovirus Differentially Exploits Cell Surface Glycosaminoglycans in a Cell Type-Dependent and MCK-2-Independent Manner. Viruses 2020, 12, 31. https://doi.org/10.3390/v12010031
Pontejo SM, Murphy PM. Mouse Cytomegalovirus Differentially Exploits Cell Surface Glycosaminoglycans in a Cell Type-Dependent and MCK-2-Independent Manner. Viruses. 2020; 12(1):31. https://doi.org/10.3390/v12010031
Chicago/Turabian StylePontejo, Sergio M, and Philip M Murphy. 2020. "Mouse Cytomegalovirus Differentially Exploits Cell Surface Glycosaminoglycans in a Cell Type-Dependent and MCK-2-Independent Manner" Viruses 12, no. 1: 31. https://doi.org/10.3390/v12010031
APA StylePontejo, S. M., & Murphy, P. M. (2020). Mouse Cytomegalovirus Differentially Exploits Cell Surface Glycosaminoglycans in a Cell Type-Dependent and MCK-2-Independent Manner. Viruses, 12(1), 31. https://doi.org/10.3390/v12010031